Recurrent parent phenome recovery analysis in six different populations with the introgression of genetic male sterile gene ms10 from hot pepper to heat tolerant bell pepper

2021 ◽  
Author(s):  
Menisha Rani ◽  
Salesh Kumar Jindal ◽  
Om Prakash Meena
Keyword(s):  
Fruit Weight ◽  
Bell Pepper ◽  
Hot Pepper ◽  
Recurrent Parent ◽  
Male Sterile ◽  
Breeding Method ◽  
Cross Combination ◽  
Successful Recovery ◽  
Backcross Breeding ◽  
Heat Tolerant

Abstract Backcrossing is one of the most commonly used breeding methods to transfer target trait(s) into a bell pepper inbreds. We attempted for the first time to transfer genetic male sterile (GMS) ms10 gene from the donor parent MS-12, popular GMS line in India, into the genetic background of six heat tolerant bell pepper inbreds, in restricted generations through the marker-assisted selection (MAS) and backcross breeding method, to develop new GMS lines in bell pepper. In this project, the recurrent parent phenome (RPP) recovery was analyzed in each generations of backcrossing using economic phenotypic markers. Phenotypic background analysis revealed that the extent of RPP recovery ranged from 43.59% (MSSM-1) to 66.26% (MSSM-21) and 70.89% (MSSM-17) to 92.09% (MSSM-21) for fruit weight, from 19.0% (MSSM-21) to 55.14% (MSSM-3) and 61.0% (MSSM-21) to 98.75% (MSSM-1) for number of lobes fruit− 1, from 55.77% (MSSM-17) to 94.25% (MSSM-2) and 69.83% (MSSM-17) to 98.26% (MSSM-3) for total fruit yield plant− 1 in BC1F1 and BC2F1 populations, respectively. Based on a paired 't' test analysis in BC2F1 population, out of six, the MSSM-1 and MSSM-2 generation was shown to be non-significant for all 19 studied traits followed by MSSM-3 (18), indicating a successful recovery of the recipient genome through marker-assisted backcross breeding after successful introgression of the ms10 gene. The GMS lines developed from this project will be utilized in bell pepper breeding programs aimed to identify the best cross-combination for their cultivation under north-Indian plains.

scholarly journals Modified backcross breeding method for rapid conversion of field corn line to shrunken2 (sh2) gene-based sweet corn line

2019 ◽  
Vol 79 (01) ◽  
Author(s):  
S. K. Jha ◽  
N. K. Singh ◽  
P. K. Agrawal
Keyword(s):  
Sweet Corn ◽  
Cost Effective ◽  
Recurrent Parent ◽  
Breeding Method ◽  
Corn Hybrids ◽  
Field Corn ◽  
Recessive Mutant ◽  
Backcross Breeding ◽  
Skilled Person ◽  
Marker Assisted Backcross Breeding

The non-availability of superior and diverse inbred parents is one of the major bottlenecks to develop high yielding sweet corn hybrids. The sh2, one of the promising recessive mutant alleles, has been used in sweet corn development however its transfer to superior field corn lines may require extra inbreeding to identify heterozygous carrier plants. The use of molecular markers linked with sh2 requires a well-equipped laboratory and skilled person to carry the marker assisted backcross breeding (MABB). Considering the above constraints, a modified backcross breeding method was used taking advantage of shrunken kernel of sweet corn for foreground selection coupled with phenotypic comparison with recurrent parent (RP). The BC1F1 plants were grown and plants having high phenotypic similarity with RP were selfed as well as backcrossed as pollen parent. The BC2F1 kernels of BC1F1 plants having high similarity with RP and segregation in BC1F2 kernels were harvested. The BC2F1 plants phenotypically most similar to recomment parameter (RP) were selfed and the shrunken kernels were selected from the segregating BC2F2 .The shrunken kernels were homozygous (sh2sh2) at Sh2 locus, which were validated upon inbreeding based on nonsegregating kernels in BC2F3 . The modified backcross method used is rapid, cost effective and can be used by maize breeder with limited resources for conversion as well diversification of sweet corn germplasm.

Backcross breeding for improvement of heat tolerance at reproductive phase in Thai rice (Oryza sativa L.) varieties

2020 ◽  
pp. 1-15
Author(s):  
C. Malumpong ◽  
R. Buadchee ◽  
B. Thammasamisorn ◽  
P. Moung-ngam ◽  
B. Wasuri ◽  
...  
Keyword(s):  
Heat Tolerance ◽  
Oryza Sativa L ◽  
Grain Filling ◽  
Dry Season ◽  
Field Conditions ◽  
Spikelet Fertility ◽  
High Yield ◽  
Recurrent Parent ◽  
Backcross Breeding ◽  
Heat Tolerant

Abstract Heat stress during the reproductive and grain-filling stages leads to severe yield losses in rice, especially in irrigated areas during the dry season in Thailand. Thus, breeding for heat-tolerant rice is one of the strategies for developing rice crops adapted to the effects of high temperature in major growing regions. M9962, a fast neutron-induced mutant, was identified as a heat-tolerant line that was then used as a donor parent and crossed with the Phisanulok 2 (PSL2) cultivar (the recurrent parent) in a backcross breeding programme. Heat tolerance scoring under controlled greenhouse and field conditions was based on the percentage of spikelet fertility. Yield trials for the BC2F7 and BC3F6 lines were subsequently conducted under field conditions at three locations and under controlled greenhouse conditions during the dry season of 2019 (DS2019). In the early generation, 210 plants of the BC1F1 population and 440 plants of BC2F1 population were evaluated for their heat tolerance, and the spikelet fertility distribution within the BC1F1, BC2F1 and BC3F1 populations showed a skewed-right pattern. In addition, most rice plants in every generation were heat sensitive. Finally, compared with PSL2, the four-candidate heat-tolerant lines exhibited a high percentage of spikelet fertility and high yield. In addition, the one candidate line, BC2F7-6-5-4-1-1-21 was most similar to PSL2 in terms of genetic background, plant type and grain quality. Thus, backcross breeding programmes via the spikelet fertility can be used as an indirect trait to select for heat tolerance.

scholarly journals 072 Backcross Breeding with RAPD Molecular Markers to Enhance Resistance to Common Bacterial Blight in Pinto Beans

HortScience ◽  
1999 ◽  
Vol 34 (3) ◽  
pp. 453E-453
Author(s):  
N. Mutlu ◽  
D.P. Coyne ◽  
S.O. Park ◽  
J.R. Steadman
Keyword(s):  
Molecular Markers ◽  
Bacterial Blight ◽  
Xanthomonas Campestris ◽  
Coat Color ◽  
Flower Color ◽  
Recurrent Parent ◽  
Common Bacterial Blight ◽  
Breeding Method ◽  
Backcross Breeding ◽  
Moderate Resistance

Common bacterial blight (CBB) in common bean (Phaseolus vulgaris L.), caused by Xanthomonas campestris pv. phaseoli (Xcp), reduces bean yields and quality throughout the world. Pinto `Chase' is a high-yielding variety with moderate resistance to Xcp derived from great northern Nebraska #1 selection 27, whose resistance is derived from an unknown tepary (P. acutifolius) bean source. XAN-159 is a black mottled small seeded breeding line with different genes for high resistance to Xcp derived from a different tepary source (PI 319443). Our objective was to pyramid different genes for Xcp resistance from the donor parent XAN-159 into the rust-resistant recurrent parent Pinto `Chase' using the classical back-cross breeding method with confirmation of resistance using RAPD molecular markers. Resistance was confirmed in some BC2F2 generation plants. Seven RAPD markers and the V locus (flower color) previously identified were confirmed in the BC1 and BC2 populations. Smaller seed size, purple flower color, and black mottled seed coat color were coinherited with resistance to Xcp. However, a recombinant plant with enhanced CBB resistance and moderate-sized pinto seed was identified. Backcross breeding is being continued.

scholarly journals Effect of Drought Stress on Capsaicin and Antioxidant Contents in Pepper Genotypes at Reproductive Stage

Plants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1286
Author(s):  
Tahir Mahmood ◽  
Rashid Mehmood Rana ◽  
Sunny Ahmar ◽  
Saima Saeed ◽  
Asma Gulzar ◽  
...  
Keyword(s):  
Drought Stress ◽  
Growth Parameters ◽  
Survival Rates ◽  
Field Capacity ◽  
Fruit Weight ◽  
Bell Pepper ◽  
Hot Pepper ◽  
Protein Activity ◽  
Formation Stage ◽  
Pepper Fruit

Pepper is one of the most important vegetables and spices in the world. Principal pungency is contributed by secondary metabolites called capsaicinoids, mainly synthesized in the placenta of pepper fruit. Various factors, including drought, limit pepper production. Flowering is one of the most sensitive stages affected by drought stress. The current study was conducted to determine the effect of drought on different pepper genotypes at the flowering and pod formation stages. Hot pepper (Pusajuala and Ghotki) and Bell pepper (Green Wonder and PPE-311) genotypes were subjected to drought (35% field capacity) at two different stages (flowering (DF) and pod formation (DP) stage). In comparison, control plants were maintained at 65% field capacity. The data regarding flowering survival rates, antioxidant protein activity, and proline content, were collected. Results indicated that parameters like flower survival percentage, number of fruits per plant, and fruit weight had significant differences among the genotypes in both treatments. A high proline level was observed in Green Wonder at the pod formation stage compared to other genotypes. Capsaicin contents of hot pepper genotypes were affected at the pod formation stage. Antioxidants like GPX were highly active (190 units) in Ghotki at pod formation. Bell pepper genotypes had a high APX activity, highly observed (100 units) in PPE-311 at pod formation, and significantly differ from hot pepper genotypes. In the catalase case, all the genotypes had the highest values in DP compared to control and DF, but Pusajuala (91 units) and Green Wonder (83 units) performed best compared to other genotypes. Overall, the results indicate that drought stress decreased reproductive growth parameters and pungency of pepper fruit as most of the plant energy was consumed in defense molecules (antioxidants). Therefore, water availability at the flowering and pod formation stage is critical to ensure good yield and pepper quality.

scholarly journals Heat-tolerant hot pepper exhibits constant photosynthesis via increased transpiration rate, high proline content and fast recovery in heat stress condition

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Sherzod Nigmatullayevich Rajametov ◽  
Eun Young Yang ◽  
Myeong Cheoul Cho ◽  
Soo Young Chae ◽  
Hyo Bong Jeong ◽  
...  
Keyword(s):  
Heat Treatment ◽  
Heat Stress ◽  
Heat Tolerance ◽  
Transpiration Rate ◽  
Stress Condition ◽  
Proline Content ◽  
Hot Pepper ◽  
Fast Recovery ◽  
Heat Stress Condition ◽  
Heat Tolerant

AbstractUnderstanding the mechanism for heat tolerance is important for the hot pepper breeding program to develop heat-tolerant cultivars in changing climate. This study was conducted to investigate physiological and biochemical parameters related to heat tolerance and to determine leaf heat damage levels critical for selecting heat-tolerant genotypes. Seedlings of two commercial cultivars, heat-tolerant ‘NW Bigarim’ (NB) and susceptible ‘Chyung Yang’ (CY), were grown in 42 °C for ten days. Photosynthesis, electrolyte conductivity, proline content were measured among seedlings during heat treatment. Photosynthetic rate was significantly reduced in ‘CY’ but not in ‘NB’ seedlings in 42 °C. Stomatal conductivity and transpiration rate was significantly higher in ‘NB’ than ‘CY’. Proline content was also significantly higher in ‘NB’. After heat treatment, leaf heat damages were determined as 0, 25, 50 and 75% and plants with different leaf heat damages were moved to a glasshouse (30–32/22–24 °C in day/night). The growth and developmental parameters were investigated until 70 days. ‘NB’ was significantly affected by leaf heat damages only in fruit yield while ‘CY’ was in fruit set, number and yield. ‘NB’ showed fast recovery after heat stress compared to ‘CY’. These results suggest that constant photosynthetic rate via increased transpiration rate as well as high proline content in heat stress condition confer faster recovery from heat damage of heat-tolerant cultivars in seedlings stages.

scholarly journals Heat-tolerant Hot Pepper Exhibits Constant Photosynthesis Via Increased 4 Transpiration Rate, High Proline Content and Fast Recovery in Heat Stress 5 Condition

2021 ◽  
Author(s):  
Sherzod Rajametov ◽  
Eun Young Yang ◽  
Myeong Cheoul Cho ◽  
Soo Young Chae ◽  
Hyo Bong Jeong ◽  
...  
Keyword(s):  
Heat Treatment ◽  
Heat Stress ◽  
Photosynthetic Rate ◽  
Heat Tolerance ◽  
Transpiration Rate ◽  
Proline Content ◽  
Hot Pepper ◽  
Fast Recovery ◽  
Heat Damage ◽  
Heat Tolerant

Understanding the mechanism for heat tolerance is important for the hot pepper breeding program to develop heat-tolerant cultivars in changing climate. This study was conducted to investigate physiological and biochemical parameters related to heat tolerance and to determine leaf heat damage levels critical for selecting heat-tolerant genotypes. Seedlings of two commercial cultivars, heat-tolerant ‘NW Bigarim’ (NB) and susceptible ‘Chyung Yang’ (CY), were grown in 42 °C for ten days. Photosynthesis, electrolyte conductivity, proline content were measured among seedlings during heat treatment. Photosynthetic rate was significantly reduced in ‘CY’ but not in ‘NB’ seedlings in 42 °C. Stomatal conductivity and transpiration rate was significantly higher in ‘NB’ than ‘CY’. Proline content was also significantly higher in ‘NB’. After heat treatment, leaf heat damages were determined as 0, 25, 50 and 75% and plants with different leaf heat damages were moved to a glasshouse (30–32/22–24 °C in day/night). The growth and developmental parameters were investigated until 70 days. ‘NB’ was significantly affected by leaf heat damages only in fruit yield while ‘CY’ was in fruit set, number and yield. ‘NB’ showed fast recovery after heat stress compared to ‘CY’. These results suggest that constant photosynthetic rate via increased transpiration rate as well as high proline content in heat stress condition confer faster recovery from heat damage of heat-tolerant cultivars in seedlings stages.

scholarly journals STUDIES ON THE VARIABILITY OF THE PRODUCTIVITY COMPONENTS IN A COLLECTION OF HOT PEPPER LANDRACES (CAPSICUM ANUUM VAR. MICROCARPUM)

2021 ◽  
Vol 10 (19) ◽  
pp. 201-208
Author(s):  
Emilian Madoşă ◽  
Lavinia Sasu ◽  
Sorin Ciulca ◽  
Constantin Avădanei ◽  
Adriana Ciulca ◽  
...  
Keyword(s):  
Morphological Characteristics ◽  
Fruit Production ◽  
Fruit Weight ◽  
Plant Production ◽  
Average Weight ◽  
Hot Pepper ◽  
Breeding Programs ◽  
Hot Peppers ◽  
Biometric Measurements ◽  
Western Romania

The aim of the research was to evaluate the variability value of the main characters that contribute to the achievement of plant production to a collection of hot pepper genotypes. The biological material was composed of 17 landraces of hot peppers collected from western Romania. The study was conducted for two years, with biometric measurements on the morphological characteristics of fruit production on the plant. Intra-population variability was assessed (mean, standard deviation of mean and coefficient of variability) and differences between populations for these characters. The results show that the variability within the collection is high. Within populations, fruit sizes (length, diameter) are uniform, but the number of fruits and their weight per plant show greater variability. Within the collection, variations in morphological characteristics are large, especially for fruit length, fruit weight, number and weight of fruit per plant. Among the landraces studied, some may be recommended for breeding programs, as parents or as material for the application of selection: for long fruits (Juliţa, Aldeşti I and Satchinez I), for short fruits (Satchinez III), but also the landraces Rieni III (17.07 g average weight of the fruit), Temerești II (89.82 fruits per plant), Aldești I (931.17 g fruits per plant).

IMPROVEMENT OF TOMATO FRUIT SIZE AND MATURITY BY BACKCROSS BREEDING

1961 ◽  
Vol 41 (1) ◽  
pp. 24-30 ◽  
Author(s):  
Chas. Walkof
Keyword(s):  
Tomato Fruit ◽  
Fruit Size ◽  
Western Canada ◽  
Research Institutions ◽  
Breeding Method ◽  
Improvement Project ◽  
Breeding Lines ◽  
Correlation Data ◽  
Late Maturity ◽  
Backcross Breeding

The backcross breeding method was applied to a co-operative tomato improvement project that involved nine research institutions in Western Canada with highly effective results. Breeding lines have been obtained from the Earlinorth × Morden BB3 cross, in which fruit size was 117.15 per cent greater and maturity equal to Earlinorth. In other lines fruit size was 44.33 per cent greater and maturity 7 days earlier than Morden BB3. Combination improvement employing correlation data for selecting the largest-fruited, earliest-ripening genotypes contributed to breeding progress. Heritability estimates indicated the selection potential of the cross. The fasciation character was found to be associated with the development of large fruit size and late maturity in certain breeding lines.

Kenyon hard red spring wheat

1991 ◽  
Vol 71 (4) ◽  
pp. 1165-1168 ◽  
Author(s):  
G. R. Hughes ◽  
P. Hucl
Keyword(s):  
Triticum Aestivum ◽  
Leaf Rust ◽  
Spring Wheat ◽  
Triticum Aestivum L ◽  
Recurrent Parent ◽  
Breeding Method ◽  
Hard Red Spring Wheat ◽  
Wide Adaptation ◽  
Cultivar Description ◽  
The University

Kenyon hard red spring wheat (Triticum aestivum L.) possesses excellent resistance to leaf rust and stem rust. Kenyon was developed using the backcross breeding method, resulting in the recovery of the maturity and wide adaptation of its recurrent parent Neepawa. Kenyon was developed at the University of Saskatchewan. Key words: Cultivar description, leaf rust, Triticum aestivum L., spring wheat

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