Analysis of internal transcribed spacer1 (ITS1) region of rDNA for genetic characterization of Paramphistomum sp

Currently, information on the occurrence and genetic characterization of Toxoplasma gondii and Neospora caninum in tissues of rabbits in China is lacking. In this study, brain and heart samples from 470 slaughtered domestic rabbits were collected in Henan Province, Central China. The occurrence rate of T. gondii and N. caninum DNA detected by nested PCR was 2.8% and 2.1%, respectively. There were no significant differences (p > 0.05) in the frequency of the two parasite infections in relation to sex, breed, and region. Three out of 13 T. gondii-positive samples were completely or partially genotyped at 11 genetic markers using PCR-RFLP, and one was identified as ToxoDB genotype #9. For N. caninum, three different sequences at the ITS1 region and two genotypes at the MS5 microsatellite locus were identified. To our knowledge, this is the first genetic characterization of N. caninum isolates from rabbits.

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Molecular genetic characterization of the cervid strain (‘northern form’) ofEchinococcus granulosus

Parasitology ◽

10.1017/s0031182000076332 ◽

1994 ◽

Vol 109 (2) ◽

pp. 215-221 ◽

Cited By ~ 98

Author(s):

J. Bowles ◽

D. Blair ◽

D. P. McManus

Keyword(s):

Genetic Characterization ◽

Molecular Genetic ◽

Rflp Analysis ◽

Domestic Sheep ◽

Rflp Pattern ◽

Its1 Region ◽

Pcr Rflp ◽

Mitochondrial Nd1 ◽

Molecular Genetic Characterization

SUMMARYWe have used a number of molecular genetic approaches to characterize the cervid strain (‘northern form’) ofEchinococcus granulosus.PCR–RFLP analysis of the nuclear ITS1 region of the rDNA repeat can readily distinguish the cervid form from other strains ofE. granulosus. The complexity of the RFLP patterns obtained suggests that a number of distinct ITS1 types are present in this strain which may represent an inter-strainE. granulosushybrid. Mitochondrial CO1 sequence of the cervid genotype was ambiguous at 18 positions and closely resembles a cluster of previously characterizedE. granulosusgenotypes, G1 (common, domestic sheep)/G2 (Tasmanian sheep)/G3 (buffalo). In contrast, mitochondrial ND1 sequence, although unique, suggests that the cervid form is most similar to strains represented by the G6 (camel)/G7 (pig) genotypes. We assume that the CO1 and ND1 sequences obtained for the cervid genotype are linked in a single mitochondrial genome although this is difficult to explain if conventional molecular genetics of mitochondrial DNA are assumed. Based on its unique ND1 sequence and ITS1 PCR–RFLP pattern, the cervid strain appears to represent a distinct genotype (designated G8) ofE. granulosus.

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Histopathological and genetic characterization of colorectal mucinous carcinoma

Gastroenterology ◽

10.1016/s0016-5085(01)80821-0 ◽

2001 ◽

Vol 120 (5) ◽

pp. A166-A166

Author(s):

S FUJII ◽

T KUSAKA ◽

T KAIHARA ◽

Y UEDA ◽

T CHIBA ◽

...

Keyword(s):

Genetic Characterization ◽

Mucinous Carcinoma

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Genetic characterization of high hyperdiploidy in childhood acute lymphoblastic leukemia

Klinische Pädiatrie ◽

10.1055/s-0029-1222694 ◽

2009 ◽

Vol 221 (03) ◽

Author(s):

R Vagkopoulou ◽

C Eckert ◽

U Ungethüm ◽

G Körner ◽

M Stanulla ◽

...

Keyword(s):

Acute Lymphoblastic Leukemia ◽

Genetic Characterization ◽

Lymphoblastic Leukemia ◽

Childhood Acute Lymphoblastic Leukemia

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TBE in Sweden

Tick-borne encephalitis - The Book ◽

10.33442/26613980_12b32-3 ◽

2020 ◽

Keyword(s):

Genetic Characterization ◽

Immunoglobulin M ◽

Enzyme Linked Immunosorbent Assay ◽

Second Phase ◽

Serum Samples ◽

Tick Borne Encephalitis ◽

Specific Immunoglobulin ◽

Tick Borne Encephalitis Virus ◽

First Time

Tick-borne encephalitis virus (TBEV) was isolated for the first time in Sweden in 1958 (from ticks and from 1 tick-borne encephalitis [TBE] patient).1 In 2003, Haglund and colleagues reported the isolation and antigenic and genetic characterization of 14 TBEV strains from Swedish patients (samples collected 1991–1994).2 The first serum sample, from which TBEV was isolated, was obtained 2–10 days after onset of disease and found to be negative for anti-TBEV immunoglobulin M (IgM) by enzyme-linked immunosorbent assay (ELISA), whereas TBEV-specific IgM (and TBEV-specific immunoglobulin G/cerebrospinal fluid [IgG/CSF] activity) was demonstrated in later serum samples taken during the second phase of the disease.

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