scholarly journals Occurrence and genetic characterization of Toxoplasma gondii and Neospora caninum in slaughtered domestic rabbits in central China

Parasite ◽  
2019 ◽  
Vol 26 ◽  
pp. 36 ◽  
Author(s):  
Weifeng Qian ◽  
Wenchao Yan ◽  
Chaochao Lv ◽  
Rongzhen Bai ◽  
Tianqi Wang
Keyword(s):  
Toxoplasma Gondii ◽  
Genetic Characterization ◽  
Neospora Caninum ◽  
Central China ◽  
Occurrence Rate ◽  
Its1 Region ◽  
Pcr Rflp ◽  
Parasite Infections ◽  
Domestic Rabbits

Currently, information on the occurrence and genetic characterization of Toxoplasma gondii and Neospora caninum in tissues of rabbits in China is lacking. In this study, brain and heart samples from 470 slaughtered domestic rabbits were collected in Henan Province, Central China. The occurrence rate of T. gondii and N. caninum DNA detected by nested PCR was 2.8% and 2.1%, respectively. There were no significant differences (p > 0.05) in the frequency of the two parasite infections in relation to sex, breed, and region. Three out of 13 T. gondii-positive samples were completely or partially genotyped at 11 genetic markers using PCR-RFLP, and one was identified as ToxoDB genotype #9. For N. caninum, three different sequences at the ITS1 region and two genotypes at the MS5 microsatellite locus were identified. To our knowledge, this is the first genetic characterization of N. caninum isolates from rabbits.

scholarly journals Molecular Detection and Genetic Characterization of Toxoplasma gondii in Farmed Minks (Neovison vison) in Northern China by PCR-RFLP

PLoS ONE ◽  
2016 ◽  
Vol 11 (11) ◽  
pp. e0165308 ◽  
Author(s):  
Wen-Bin Zheng ◽  
Xiao-Xuan Zhang ◽  
Jian-Gang Ma ◽  
Fa-Cai Li ◽  
Quan Zhao ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Molecular Detection ◽  
Genetic Characterization ◽  
Northern China ◽  
Neovison Vison ◽  
Pcr Rflp

scholarly journals Genetic characterization of Toxoplasma gondii in Yunnan black goats (Capra hircus) in southwest China by PCR-RFLP

2015 ◽  
Vol 8 (1) ◽  
pp. 57 ◽  
Author(s):  
Qiang Miao ◽  
Si-Yang Huang ◽  
Si-Yuan Qin ◽  
Xin Yu ◽  
Yan Yang ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Southwest China ◽  
Genetic Characterization ◽  
Capra Hircus ◽  
Pcr Rflp

Molecular genetic characterization of the cervid strain (‘northern form’) ofEchinococcus granulosus

Parasitology ◽  
1994 ◽  
Vol 109 (2) ◽  
pp. 215-221 ◽  
Author(s):  
J. Bowles ◽  
D. Blair ◽  
D. P. McManus
Keyword(s):  
Genetic Characterization ◽  
Molecular Genetic ◽  
Rflp Analysis ◽  
Domestic Sheep ◽  
Rflp Pattern ◽  
Its1 Region ◽  
Pcr Rflp ◽  
Mitochondrial Nd1 ◽  
Molecular Genetic Characterization

SUMMARYWe have used a number of molecular genetic approaches to characterize the cervid strain (‘northern form’) ofEchinococcus granulosus.PCR–RFLP analysis of the nuclear ITS1 region of the rDNA repeat can readily distinguish the cervid form from other strains ofE. granulosus. The complexity of the RFLP patterns obtained suggests that a number of distinct ITS1 types are present in this strain which may represent an inter-strainE. granulosushybrid. Mitochondrial CO1 sequence of the cervid genotype was ambiguous at 18 positions and closely resembles a cluster of previously characterizedE. granulosusgenotypes, G1 (common, domestic sheep)/G2 (Tasmanian sheep)/G3 (buffalo). In contrast, mitochondrial ND1 sequence, although unique, suggests that the cervid form is most similar to strains represented by the G6 (camel)/G7 (pig) genotypes. We assume that the CO1 and ND1 sequences obtained for the cervid genotype are linked in a single mitochondrial genome although this is difficult to explain if conventional molecular genetics of mitochondrial DNA are assumed. Based on its unique ND1 sequence and ITS1 PCR–RFLP pattern, the cervid strain appears to represent a distinct genotype (designated G8) ofE. granulosus.

scholarly journals Genetic Characterization of Toxoplasma gondii from Wild Rodents in Sichuan Province, Southwestern China

2019 ◽  
Author(s):  
XinLei WANG ◽  
Ling DONG ◽  
Li ZHANG ◽  
Yan LV ◽  
Qian LI ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Toxoplasma Gondii ◽  
Nested Pcr ◽  
Genetic Characterization ◽  
Sichuan Province ◽  
Southwestern China ◽  
Wild Rodents ◽  
New Genotype ◽  
Pcr Rflp

Background: Wild rodents are the intermediate hosts of Toxoplasma gondii. The distribution of genetic diversity of T. gondii in wild rodents is of importance to understand the transmission of this parasite. This study aimed to genetically characterize T. gondii isolates from wild rodents in Sichuan province, southwestern China in 2013. Methods: Genomic DNA was extracted from 10 g wild rodents’ brain samples. Semi-nested PCR and multilocous PCR-RFLP technology were performed to examine genetic diversity of T. gondii isolates as described previously. Results: Overall, 181 brain tissues of different wild rodents, including Eothenomys miletus (n=88), Crocidura attenuate (n=9), Rattus rattus sladeni (n=46), Mus musculus Linnaeus (n=6) and R. niviventer (n=32) were tested for T. gondii DNA, respectively. Six of them were positive for the T. gondii B1 gene by semi-nested PCR amplification, 4 showed complete genotyping results for all 11 polymorphic loci (SAG1, SAG2, alt. SAG2, SAG3, BTUB, GRA6, L358, PK1, C22-8, C29-2 and Apico) by PCR-RFLP, determined to represent a potential new genotype (http://toxodb.org/toxo/). Conclusion: These results documented genetic characterization of T. gondii in wild rodents from Sichuan province, and enriched the genetic diversity of T. gondii in China.

scholarly journals Prevalence and Genetic Characterization of <I>Toxoplasma gondii</I> in Pet Dogs in Central China

2015 ◽  
Vol 53 (1) ◽  
pp. 125-128 ◽  
Author(s):  
Wei-Feng Qian ◽  
Wen-Chao Yan ◽  
Tian-Qi Wang ◽  
Kai Zhai ◽  
Li-Fang Han ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Genetic Characterization ◽  
Central China ◽  
Pet Dogs

Prevalence and genetic characterization of Toxoplasma gondii in badgers ( Melogale moschata ) in southern China by PCR-RFLP

2017 ◽  
Vol 52 ◽  
pp. 30-33 ◽  
Author(s):  
Kai Chen ◽  
Si-Yang Huang ◽  
Jin-Lei Wang ◽  
Rong-Liang Hu ◽  
Qiu-Xia Yao ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Genetic Characterization ◽  
Southern China ◽  
Pcr Rflp

scholarly journals Genetic characterization of Toxoplasma gondii from pigs from different localities in China by PCR-RFLP

2013 ◽  
Vol 6 (1) ◽  
pp. 227 ◽  
Author(s):  
Hai-Hai Jiang ◽  
Si-Yang Huang ◽  
Dong-Hui Zhou ◽  
Xiao-Xuan Zhang ◽  
Chunlei Su ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Genetic Characterization ◽  
Pcr Rflp

scholarly journals Isolation and genetic characterization of Toxoplasma gondii from free-ranging and captive birds and mammals in Pernambuco state, Brazil

2018 ◽  
Vol 27 (4) ◽  
pp. 481-487 ◽  
Author(s):  
Marcio André Silva ◽  
Hilda Fátima Jesus Pena ◽  
Herbert Sousa Soares ◽  
Juliana Aizawa ◽  
Solange Oliveira ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Genetic Characterization ◽  
Genetic Population ◽  
Wild Mammals ◽  
Genotypic Characterization ◽  
Pcr Rflp ◽  
Dna Fragment ◽  
Free Ranging ◽  
Lontra Longicaudis

Abstract Recent genetic population studies on Toxoplasma gondii in Brazil have shown large genetic variability. The objective of the present study was to isolate and genotypically characterize T. gondii from free-ranging and captive wild mammals and birds in Pernambuco state, Brazil. Fragments of heart, brain, skeletal muscle and diaphragm tissue from 71 birds and 34 mammals, which were either free-ranging or captive, were collected. Samples from 32 of these animals were subjected to bioassays in mice. Samples from the remaining 73 animals underwent biomolecular diagnosis, using PCR technique, targeting a repetitive DNA fragment of 529 bp in T. gondii. A non-virulent isolate (TgButstBrPE1) was obtained from a free-ranging striated heron (Butorides striata) and, based on primary samples, seven animals were found to be positive. The primary samples and the isolate obtained were subjected to PCR-RFLP using the markers SAG1, 5’3’SAG2, alt.SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, Apico and CS3. ToxoDB-RFLP genotype #13 from the striated heron isolate and Type BrIII genotype from a captive otter ( Lontra longicaudis) (PS-TgLonloBrPE1) were obtained. The present study describes the first isolation and genotypic characterization of T. gondii in free-ranging striated heron, and the first genotypic characterization of T. gondii in a captive otter.

Cytologic detection of Toxoplasma gondii in the cerebrospinal fluid of a dog and in vitro isolation of a unique mouse-virulent recombinant strain

2021 ◽  
pp. 104063872199668
Author(s):  
Waléria Borges-Silva ◽  
Mariana M. Rezende-Gondim ◽  
Gideão S. Galvão ◽  
Daniele S. Rocha ◽  
George R. Albuquerque ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Toxoplasma Gondii ◽  
Recombinant Strain ◽  
Neospora Caninum ◽  
Clinical Signs ◽  
Species Identity ◽  
Cytologic Examination ◽  
Pcr Rflp ◽  
Tissue Homogenates

Parasites resembling Neospora caninum or Toxoplasma gondii were detected by cytologic examination of cerebrospinal fluid (CSF) from a dog with neurologic disease. The dog became severely ill and was euthanized. Canine tissue homogenates were used for direct parasite isolation in cell culture, bioassay in 2 mouse lineages, and PCR. T. gondii was isolated in monkey kidney cells, and species identity was confirmed by PCR. Inoculated parasites were highly virulent for mice, which developed clinical signs and were euthanized immediately. PCR-RFLP for T. gondii using the cultured isolate (TgDgBA22) was conducted with 12 genetic markers, and a unique recombinant strain was identified. Detection of T. gondii by CSF cytology, although described in humans, had not been reported previously in dogs, to our knowledge, and was crucial for the diagnosis of toxoplasmosis in the examined dog.

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