Cu-mediated NIR photoinduced polymerization for highly sensitive electrochemical nucleic acid detection

Objective To explore the usefulness of highly sensitive nucleic acid detection for assisting with the accurate antiviral treatment of patients with cirrhosis that was caused by hepatitis. Methods There were 377 patients with hepatitis B with cirrhosis and 119 patients with hepatitis C with cirrhosis, either as hospitalized patients and outpatients, who were enrolled into the study. Among them, 299 were men and 197 were women between 23 and 82 years of age. All patients were examined using a domestic HBV DNA/HCV RNA test, which was negative in 162 cirrhosis with hepatitis B and 54 cirrhosis with hepatitis C patients (HBV DNA/HCV RNA <500 IU/mL). Prediction and analysis of the HBV DNA load using alanine aminotransferase (ALT) level was based on receiver operating characteristics (ROC) curve analysis. Results For patients with hepatitis C with cirrhosis, after the antiviral therapy, ALT, HCV RNA, and Child–Pugh grade were significantly improved compared with before treatment. ROC analysis results showed that an ALT level of 29 IU/mL was the most sensitive cutoff value to judge a positive HBV DNA load (sensitivity 1.0, specificity 0.237, Youden index 0.763). Conclusion Precise detection for patients with cirrhosis caused by hepatitis is required for accurate therapy.

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Solid-phase PCR based on thermostable, encoded magnetic microspheres for simple, highly sensitive and multiplexed nucleic acid detection

Sensors and Actuators B Chemical ◽

10.1016/j.snb.2019.126953 ◽

2019 ◽

Vol 298 ◽

pp. 126953

Author(s):

Zhejia Gu ◽

Simin Zhao ◽

Gaolian Xu ◽

Cang Chen ◽

Yao Wang ◽

...

Keyword(s):

Nucleic Acid ◽

Solid Phase ◽

Magnetic Microspheres ◽

Nucleic Acid Detection ◽

Highly Sensitive

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CRISPR-based surveillance for COVID-19 using genomically-comprehensive machine learning design

10.1101/2020.02.26.967026 ◽

2020 ◽

Cited By ~ 41

Author(s):

Hayden C. Metsky ◽

Catherine A. Freije ◽

Tinna-Solveig F. Kosoko-Thoroddsen ◽

Pardis C. Sabeti ◽

Cameron Myhrvold

Keyword(s):

Nucleic Acid ◽

Clinical Presentation ◽

Detection System ◽

Genomic Diversity ◽

Nucleic Acid Detection ◽

Learning Design ◽

Highly Sensitive ◽

Global Concern ◽

Ongoing Surveillance ◽

Flow Detection

AbstractThe emergence and outbreak of SARS-CoV-2, the causative agent of COVID-19, has rapidly become a global concern and has highlighted the need for fast, sensitive, and specific tools to surveil circulating viruses. Here we provide assay designs and experimental resources, for use with CRISPR-based nucleic acid detection, that could be valuable for ongoing surveillance. We provide assay designs for detection of 67 viral species and subspecies, including: SARS-CoV-2, phylogenetically-related viruses, and viruses with similar clinical presentation. The designs are outputs of algorithms that we are developing for rapidly designing nucleic acid detection assays that are comprehensive across genomic diversity and predicted to be highly sensitive and specific. Of our design set, we experimentally screened 4 SARS-CoV-2 designs with a CRISPR-Cas13 detection system and then extensively tested the highest-performing SARS-CoV-2 assay. We demonstrate the sensitivity and speed of this assay using synthetic targets with fluorescent and lateral flow detection. Moreover, our provided protocol can be extended for testing the other 66 provided designs. Assay designs are available at https://adapt.sabetilab.org/.

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