Ultrasensitive detection and risk assessment of di(2-ethylhexyl) phthalate migrated from daily-use plastic products using a nanostructured electrochemical aptasensor

Herein, we describe a novel homogenous electrochemical aptasensor for the ultrasensitive detection of ractopamine (RAC) based on the signal amplification of a hairpin DNA cascade amplifier (HDCA) and electrocatalysis of dsDNA-templated copper nanoparticles.

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A signal-on electrochemical aptasensor for ultrasensitive detection of endotoxin using three-way DNA junction-aided enzymatic recycling and graphene nanohybrid for amplification

Nanoscale ◽

10.1039/c3nr05930h ◽

2014 ◽

Vol 6 (5) ◽

pp. 2902 ◽

Cited By ~ 72

Author(s):

Lijuan Bai ◽

Yaqin Chai ◽

Xiaoyun Pu ◽

Ruo Yuan

Keyword(s):

Ultrasensitive Detection ◽

Electrochemical Aptasensor

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Overcompensation of CoA Trapping by Di(2-ethylhexyl) Phthalate (DEHP) Metabolites in Livers of Wistar Rats

International Journal of Molecular Sciences ◽

10.3390/ijms222413489 ◽

2021 ◽

Vol 22 (24) ◽

pp. 13489

Author(s):

David Hala ◽

Lene H. Petersen ◽

Duane B. Huggett ◽

Michelle A. Puchowicz ◽

Henri Brunengraber ◽

...

Keyword(s):

Wistar Rats ◽

Metabolic Activation ◽

Peroxisome Proliferator ◽

Liquid Chromatography Mass Spectrometry ◽

Peroxisome Proliferator Activated Receptor ◽

Malonyl Coa ◽

Male Wistar Rats ◽

Ethylhexyl Phthalate ◽

Dehp Exposure ◽

Plastic Products

Di(2-ethylhexyl) phthalate (DEHP) is commonly used as a plasticizer in various industrial and household plastic products, ensuring widespread human exposures. Its routine detection in human bio-fluids and the propensity of its monoester metabolite to activate peroxisome proliferator activated receptor-α (PPARα) and perturb lipid metabolism implicate it as a metabolic disrupter. In this study we evaluated the effects of DEHP exposure on hepatic levels of free CoA and various CoA esters, while also confirming the metabolic activation to CoA esters and partial β-oxidation of a DEHP metabolite (2-ethyhexanol). Male Wistar rats were exposed via diet to 2% (w/w) DEHP for fourteen-days, following which hepatic levels of free CoA and various CoA esters were identified using liquid chromatography-mass spectrometry. DEHP exposed rats showed significantly elevated free CoA and increased levels of physiological, DEHP-derived and unidentified CoA esters. The physiological CoA ester of malonyl-CoA and DEHP-derived CoA ester of 3-keto-2-ethylhexanoyl-CoA were the most highly elevated, at eighteen- and ninety eight-times respectively. We also detected sixteen unidentified CoA esters which may be derivative of DEHP metabolism or induction of other intermediary metabolism metabolites. Our results demonstrate that DEHP is a metabolic disrupter which affects production and sequestration of CoA, an essential cofactor of oxidative and biosynthetic reactions.

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Risk Assessment of Phthalate Compounds in Bottled Water Consumed in Isfahan, Iran

Journal of Food Quality and Hazards Control ◽

10.18502/jfqhc.8.2.6470 ◽

2021 ◽

Author(s):

F. Esteki ◽

H. Karimi ◽

M. Moazeni ◽

Z. Esfandiari ◽

M. Zarean ◽

...

Keyword(s):

Public Health ◽

Risk Assessment ◽

Water Samples ◽

Cross Sectional Study ◽

Bottled Water ◽

Gas Chromatography Mass Spectrometry ◽

Sectional Study ◽

Cross Sectional ◽

Pet Bottles ◽

Ethylhexyl Phthalate

Background: Phthalates are main ingredients of polyethylene terephthalate (PET) bottles used for storage of water. These compounds can cause adverse effects on human health. The purpose of this study was the quantification of the amounts of phthalates migrated in bottled water as well as the risk assessment of those compounds. Methods: This cross sectional study was performed on 15 PET bottled water samples of popular brands distributed in Isfahan city, Iran. The samples were immediately sent to laboratory for analysis. Amounts of Phthalic Anhydride (PA), di-2 ethylhexyl phthalate (DEHP), di-buthyl phthalate (DBP), di-ethyl phthalate (DEP), and terphthalic acid (TPA) in bottled water samples were measured using Gas Chromatography-Mass Spectrometry (GC-MS) analysis. Risk assessment of migrated compounds were calculated and com- pared with Hazard Quito (HQ) standards. Results: The ranges of PA, DEHP, DBP, and TPA were 2.3-26, 171-845, 30-2251, and 24-657 ppb, respectively. DEP was not found in none of the analyzed samples. HQ levels of DEHP, TPA, and DBP were more than 1 in bottled water. Conclusion: In the current study, the concentration of DEHP, TPA, and DBP in some bottled water of Isfahan, Iran were higher than the permitted limits. Also, HQ levels of these three migrated compounds were more than 1 in bottled water that is alarming for public health in this region of Iran.

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