173. Neural Precursor Cells Produce Trophic Factors In Vitro and Following In Vivo Transplantation into the Injured Spinal Cord

2008 ◽  
Vol 8 (5) ◽  
pp. 88S
Author(s):  
Gregory Hawryluk ◽  
Eftekhar Eftekharpour ◽  
Soheila Karimi-Abdolrezaee ◽  
Michael Fehlings
Keyword(s):  
Spinal Cord ◽  
Precursor Cells ◽  
Neural Precursor Cells ◽  
Trophic Factors ◽  
Neural Precursor ◽  
Injured Spinal Cord

Adult human spinal cord harbors neural precursor cells that generate neurons and glial cells in vitro

2008 ◽  
Vol 86 (9) ◽  
pp. 1916-1926 ◽  
Author(s):  
C. Dromard ◽  
H. Guillon ◽  
V. Rigau ◽  
C. Ripoll ◽  
J.C. Sabourin ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Glial Cells ◽  
Precursor Cells ◽  
Neural Precursor Cells ◽  
Neural Precursor ◽  
Human Spinal Cord ◽  
Adult Human

scholarly journals Conserved fate and function of ferumoxides-labeled neural precursor cells in vitro and in vivo

2009 ◽  
pp. NA-NA ◽  
Author(s):  
Mikhal E. Cohen ◽  
Naser Muja ◽  
Nina Fainstein ◽  
Jeff W.M. Bulte ◽  
Tamir Ben-Hur
Keyword(s):  
Precursor Cells ◽  
Neural Precursor Cells ◽  
Neural Precursor ◽  
And Function

scholarly journals Three Growth Factors Induce Proliferation and Differentiation of Neural Precursor Cells In Vitro and Support Cell-Transplantation after Spinal Cord Injury In Vivo

2020 ◽  
Vol 2020 ◽  
pp. 1-15
Author(s):  
Alexander Younsi ◽  
Guoli Zheng ◽  
Moritz Scherer ◽  
Lennart Riemann ◽  
Hao Zhang ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Growth Factors ◽  
Financial Burden ◽  
Precursor Cells ◽  
Neural Precursor Cells ◽  
Neural Precursor ◽  
Cord Injury ◽  
Proliferation And Differentiation

Stem cell therapy with neural precursor cells (NPCs) has the potential to improve neuroregeneration after spinal cord injury (SCI). Unfortunately, survival and differentiation of transplanted NPCs in the injured spinal cord remains low. Growth factors have been successfully used to improve NPC transplantation in animal models, but their extensive application is associated with a relevant financial burden and might hinder translation of findings into the clinical practice. In our current study, we assessed the potential of a reduced number of growth factors in different combinations and concentrations to increase proliferation and differentiation of NPCs in vitro. After identifying a “cocktail” (EGF, bFGF, and PDGF-AA) that directed cell fate towards the oligodendroglial and neuronal lineage while reducing astrocytic differentiation, we translated our findings into an in vivo model of cervical clip contusion/compression SCI at the C6 level in immunosuppressed Wistar rats, combining NPC transplantation and intrathecal administration of the growth factors 10 days after injury. Eight weeks after SCI, we could observe surviving NPCs in the injured animals that had mostly differentiated into oligodendrocytes and oligodendrocytic precursors. Moreover, “Stride length” and “Average Speed” in the CatWalk gait analysis were significantly improved 8 weeks after SCI, representing beneficial effects on the functional recovery with NPC transplantation and the administration of the three growth factors. Nevertheless, no effects on the BBB scores could be observed over the course of the experiment and regeneration of descending tracts as well as posttraumatic myelination remained unchanged. However, reactive astrogliosis, as well as posttraumatic inflammation and apoptosis was significantly reduced after NPC transplantation and GF administration. Our data suggest that NPC transplantation is feasible with the use of only EGF, bFGF, and PDGF-AA as supporting growth factors.

Functional motor neurons differentiating from mouse multipotent spinal cord precursor cells in culture and after transplantation into transected sciatic nerve

2003 ◽  
Vol 98 (5) ◽  
pp. 1094-1103 ◽  
Author(s):  
Stephen C. MacDonald ◽  
Ian G. Fleetwood ◽  
Shawn Hochman ◽  
Janice G. Dodd ◽  
Gavin K. W. Cheng ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Sciatic Nerve ◽  
Motor Neurons ◽  
Precursor Cells ◽  
Neural Precursor Cells ◽  
Trophic Factors ◽  
Neural Precursor ◽  
Content Type ◽  
Islet 1 ◽  
Fine Print

Object. One of the current challenges in neurobiology is to ensure that neural precursor cells differentiate into specific neuron types, so that they can be used for transplantation purposes in patients with neuron loss. The goal of this study was to determine if spinal cord precursor cells could differentiate into motor neurons both in culture and following transplantation into a transected sciatic nerve. Methods. In cultures with trophic factors, neurons differentiate from embryonic precursor cells and express motor neuronal markers such as choline acetyltransferase (ChAT), Islet-1, and REG2. Reverse transcription—polymerase chain reaction analysis has also demonstrated the expression of Islet-1 in differentiated cultures. A coculture preparation of neurospheres and skeletal myocytes was used to show the formation of neuromuscular connections between precursor cell—derived neurons and myocytes both immunohistochemically and electrophysiologically. Following various survival intervals, precursor cells transplanted distal to a transection of the sciatic nerve differentiated into neurons expressing the motor neuron markers ChAT and the α11.2 (class C, L-type) voltage-sensitive Ca++ channel subunit. These cells extended axons into the muscle, where they formed cholinergic terminals. Conclusions. These results demonstrate that motor neurons can differentiate from spinal cord neural precursor cells grown in culture as well as following transplantation into a transected peripheral nerve.

In vitro differentiation of human bone marrow stromal cells into neural precursor cells using small molecules

2021 ◽  
Vol 363 ◽  
pp. 109340
Author(s):  
Abeer Sallam ◽  
Thangirala Sudha ◽  
Noureldien H.E. Darwish ◽  
Samar Eghotny ◽  
Abeer E-Dief ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Small Molecules ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Precursor Cells ◽  
Marrow Stromal Cells ◽  
Neural Precursor Cells ◽  
Neural Precursor ◽  
In Vitro Differentiation

scholarly journals Microstructure and Mechanical Properties of PU/PLDL Sponges Intended for Grafting Injured Spinal Cord

Polymers ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 2693
Author(s):  
Anna Lis-Bartos ◽  
Dariusz Szarek ◽  
Małgorzata Krok-Borkowicz ◽  
Krzysztof Marycz ◽  
Włodzimierz Jarmundowicz ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Spinal Cord ◽  
Young Modulus ◽  
In Vivo Studies ◽  
Polymer Composition ◽  
In Vitro Toxicity ◽  
Injured Spinal Cord ◽  
Degradation Rates

Highly porous, elastic, and degradable polyurethane and polyurethane/polylactide (PU/PLDL) sponges, in various shapes and sizes, with open interconnected pores, and porosity up to 90% have been manufactured. They have been intended for gap filling in the injured spinal cord. The porosity of the sponges depended on the content of polylactide, i.e., it decreased with the increase of polylactide content. The rise of polylactide content caused an increase of Young modulus and rigidity as well as a more complex morphology of the polyurethane/polylactide blends. The mechanical properties, in vitro toxicity, and degradation in artificial cerebrospinal fluid were tested. Sponges underwent continuous degradation with varying degradation rates depending on the polymer composition. In vitro cell studies with fibroblast cultures proved the biocompatibility of the polymers. Based on the obtained results, the designed PU/PLDL sponges appeared to be promising candidates for bridging gaps within injured spinal cord in further in vitro and in vivo studies.

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