Hydrophobic ion pairing to enhance encapsulation of water-soluble additives into CO2-swollen polymer microparticles

Tetramethylpyrazine (TMP), isolated from the rhizome of the traditional Chinese medicinal plant Chuanxiong (Ligusticum wallichii Franchat) shows therapeutic efficacy against osteoarthritis. After intra-articular injection, the retention time of TMP in the joint cavity is short, which limits its treatment effect. To avoid this problem, the present study explored the preparation of a TMP nanosuspension (TMP-NS) based on hydrophobic ion pairing. TMP-NS showed a particle size of approximately 588 nm and, after intra-articular injection in rats, it had longer retention in the articular cavity, higher TMP concentrations in joints, and greater anti-osteoarthritic efficacy than TMP solution. TMP-NS didn’t cause significant inflammation at the joint. These results suggest that TMP-NS may strengthen and prolong the therapeutic efficacy of TMP against osteoarthritis without systemic toxicity.

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Novel skin penetrating berberine oleate complex capitalizing on hydrophobic ion pairing approach

International Journal of Pharmaceutics ◽

10.1016/j.ijpharm.2018.07.051 ◽

2018 ◽

Vol 549 (1-2) ◽

pp. 76-86 ◽

Cited By ~ 12

Author(s):

Abrar S. Torky ◽

May S. Freag ◽

Maha M.A. Nasra ◽

Ossama Y. Abdallah

Keyword(s):

Ion Pairing ◽

Hydrophobic Ion Pairing

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Development of an Ion-Pairing Reagent and HPLC-UV Method for the Detection and Quantification of Six Water-Soluble Vitamins in Animal Feed

International Journal of Analytical Chemistry ◽

10.1155/2016/8357358 ◽

2016 ◽

Vol 2016 ◽

pp. 1-6 ◽

Cited By ~ 2

Author(s):

Ho Jin Kim

Keyword(s):

High Performance ◽

Animal Feed ◽

Ion Pairing ◽

Uv Spectra ◽

Water Soluble ◽

Array Detector ◽

Simple Method ◽

Accuracy And Precision ◽

Photodiode Array Detector ◽

Chromatographic Peaks

A novel and simple method for detecting six water-soluble vitamins in animal feed using high performance liquid chromatography equipped with a photodiode array detector (HPLC/PDA) and ion-pairing reagent was developed. The chromatographic peaks of the six water-soluble vitamins were successfully identified by comparing their retention times and UV spectra with reference standards. The mobile phase was composed of buffers A (5 mM PICB-6 in 0.1% CH3COOH) and B (5 mM PICB-6 in 65% methanol). All peaks were detected using a wavelength of 270 nm. Method validation was performed in terms of linearity, sensitivity, selectivity, accuracy, and precision. The limits of detection (LODs) for the instrument employed in these experiments ranged from 25 to 197 μg/kg, and the limits of quantification (LOQs) ranged from 84 to 658 μg/kg. Average recoveries of the six water-soluble vitamins ranged from 82.3% to 98.9%. Method replication resulted in intraday and interday peak area variation of <5.6%. The developed method was specific and reliable and is therefore suitable for the routine analysis of water-soluble vitamins in animal feed.

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