Evaluation of phototoxicity of tattoo pigments using the 3 T3 neutral red uptake phototoxicity test and a 3D human reconstructed skin model

2020 ◽  
Vol 65 ◽  
pp. 104813
Author(s):  
So Young Kim ◽  
Sowoon Seo ◽  
Ki Hwan Choi ◽  
Jaesuk Yun
Keyword(s):  
Neutral Red ◽  
Skin Model ◽  
Reconstructed Skin ◽  
Neutral Red Uptake

The EYTEX™ System and the Neutral Red Uptake Inhibition Assay in Cultured Hep G2 Cells as Alternative Methods for In Vivo Eye Irritation Following the EEC Protocol

1990 ◽  
Vol 17 (4) ◽  
pp. 325-333
Author(s):  
Paul J. Dierickx ◽  
Virginia C. Gordon
Keyword(s):  
Neutral Red ◽  
Alternative Methods ◽  
Inhibition Assay ◽  
Hep G2 ◽  
Hep G2 Cells ◽  
Eye Irritation ◽  
Uptake Inhibition ◽  
In Vitro Methods ◽  
Neutral Red Uptake

The neutral red uptake inhibition assay and the EYTEX™ system were investigated as alternative methods for the assessment of eye irritation, determined according to the EEC protocol. The 17 test chemicals used were mainly organic solvents. The xenobiotics were applied to Hep G2 cells for 24 hours at different concentrations. Neutral red uptake inhibition was then measured. The results are expressed as the NI50 value, which is the concentration of test compound required to induce a 50% reduction in neutral red uptake. The same chemicals were also tested as coded samples by the EYTEX™ test according to the manufacturer's directions. A nearly identical quantitative correlation was found for both in vitro methods with corneal opacity scores: r = 0.84 for EYTEX™ scores and r = 0.83 for log NI50, expressed in μg/ml. Whilst these correlations are certainly not perfect, it is clear that both in vitro methods can be used as valuable prescreening methods.

Molecular characterization of reconstructed skin model by Raman microspectroscopy: Comparison with excised human skin

Biopolymers ◽  
2007 ◽  
Vol 87 (4) ◽  
pp. 261-274 ◽  
Author(s):  
Ali Tfayli ◽  
Olivier Piot ◽  
Florence Draux ◽  
Franck Pitre ◽  
Michel Manfait
Keyword(s):  
Molecular Characterization ◽  
Human Skin ◽  
Raman Microspectroscopy ◽  
Skin Model ◽  
Reconstructed Skin

scholarly journals Biological Innocuousness of a Device developed to select the Optimum Spermatozoa to be used in the Treatment of Infertility

2021 ◽  
Author(s):  
Marisa A Cubilla ◽  
Hector A Guidobaldi ◽  
Laura C Giojalas
Keyword(s):  
Colony Formation ◽  
Physiological State ◽  
Neutral Red ◽  
Sperm Selection ◽  
Neutral Red Uptake

The sperm selection assay (SSA) is a method based on chemotaxis to obtain spermatozoa at the optimum physiological state to successfully fertilize the egg. It consists of a device made of acrylic and an attractant solution which includes progesterone. We evaluate potential cytotoxicity interactions by means of Neutral Red uptake, the MTT and colony formation assays, according to ISO normative. Here we showed that even stressing the conditions of the assays, the SSA device alone or together with the progesterone solutions employed, showed to be innocuous for the cells. Suggesting that SSA could be incorporated into the ART procedures.

The effect of hexaaza-and hexathia–macrocyclic ligands on transition metal cytotoxicity in human hepatoma-derived cultured cells

2002 ◽  
Vol 21 (8) ◽  
pp. 421-427 ◽  
Author(s):  
P W Smet ◽  
T F Pauwels ◽  
P J Dierickx
Keyword(s):  
Transition Metal ◽  
Cultured Cells ◽  
Copper Ions ◽  
Transition Metal Ions ◽  
Neutral Red ◽  
Macrocyclic Ligands ◽  
Human Hepatoma ◽  
Uptake Inhibition ◽  
Copper Zinc ◽  
Neutral Red Uptake

The effect of macrocyclic ligands on cytotoxic concentrations of the transition metal ions of copper, zinc, and cadmium was investigated. For this purpose, a hexaaza-[3,6,9,17,20,23-hexaazatricyclo[23.3.1.111,15] triaconta–1(29),11(30),12,14,25,27–hexaene (L2)] and hexathia-chelating ligand [1,4,7,10,13,16-hexathiacyclooctadecane (L3)] were used in the human hepatoma-derived HepG2 cell line. The cytotoxicity was measured by the neutral red uptake inhibition assay. First, the NI50 of the ligands, i.e., the concentration of the ligand inducing a 50% inhibition in neutral red uptake compared to control cells, was determined. In several metal/ligand combination experiments, the effects for L2 were difficult to interpret, whereas for L3 in combination with copper ions, a severe increase–and for zinc ions, a significant decrease of cell toxicity–relative to the metal control was observed. To further examine the different effects observed with L3 in combination with, respectively, Cu2+ and Zn2+, the glutathione (GSH) content was measured. The relative GSH content decreased as the concentration of L3 increased. It was proposed that the increased toxicity of the combination Cu2+ /L3 could be caused by the depletion of GSH and a subsequent inability to scavenge the produced reactive oxygen species (ROS). This hypothesis was supported by experiments during which vitamin E or C was added to the Cu2+ / L3 system.

In vitro stimulation of synthesis of key DEJ constituents in a reconstructed skin model: a quantitative study

2003 ◽  
Vol 25 (1-2) ◽  
pp. 55-62 ◽  
Author(s):  
C. Reymermier ◽  
A. Guezennec ◽  
J. E. Branka ◽  
J. Guesnet ◽  
E. Perrier
Keyword(s):  
Quantitative Study ◽  
Skin Model ◽  
Reconstructed Skin ◽  
Stimulation Of
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