Neutral red uptake by corneal epithelial cells and by injury-activated corneal stromal cells

1971 ◽  
Vol 11 (1) ◽  
pp. 57-IN5 ◽  
Author(s):  
Virginia L. Weimar
Keyword(s):  
Epithelial Cells ◽  
Stromal Cells ◽  
Neutral Red ◽  
Corneal Epithelial Cells ◽  
Corneal Epithelial ◽  
Neutral Red Uptake

scholarly journals Microgrooved collagen-based corneal scaffold for promoting collective cell migration and antifibrosis

RSC Advances ◽  
2019 ◽  
Vol 9 (50) ◽  
pp. 29463-29473 ◽  
Author(s):  
Sijia Xiong ◽  
Huichang Gao ◽  
Lanfeng Qin ◽  
Yongguang Jia ◽  
Meng Gao ◽  
...  
Keyword(s):  
Cell Migration ◽  
Epithelial Cells ◽  
Stromal Cells ◽  
Collagen Membrane ◽  
Collective Cell Migration ◽  
Corneal Epithelial Cells ◽  
Corneal Epithelial

Microgrooved collagen membrane can effectively promote the epithelialization of corneal epithelial cells and inhibit the fibrosis of corneal stromal cells.

scholarly journals In Vivo Confocal Microscopy of Cornea in Patients with Terrien’s Marginal Corneal Degeneration

2019 ◽  
Vol 2019 ◽  
pp. 1-8 ◽  
Author(s):  
Ting Chen ◽  
Qiangxiang Li ◽  
Xiangbo Tang ◽  
Min Liao ◽  
Hua Wang
Keyword(s):  
Epithelial Cells ◽  
Confocal Microscopy ◽  
Stromal Cells ◽  
Nerve Fibers ◽  
In Vivo Confocal Microscopy ◽  
Corneal Epithelial Cells ◽  
Corneal Epithelial ◽  
Department Of Ophthalmology ◽  
Severity Of The Disease

This study was aimed at observing the morphological changes of the cornea with ocular in vivo confocal microscopy (IVCM) in patients with Terrien’s marginal degeneration (TMD). Ten patients (20 eyes) with TMD treated in the Department of Ophthalmology, Xiangya Hospital, and 10 healthy controls (20 eyes) were included in the current study. A detailed slit lamp microscopy, anterior segment photography, and corneal IVCM examination were performed for each eye. The density of central and marginal corneal epithelial cells, stromal cells, and subepithelial nerve fibers was compared between the two groups using the Wilcoxon rank sum test. Compared with the control group, the corneal epithelial and endothelial cells in the TMD group showed granular highly reflective substances and thinner subepithelial nerve fibers. The uneven dot-like highly reflective substances without cell structures appeared in the stromal layer of the cornea. The density of central and marginal corneal epithelial cells, stromal cells, and subepithelial nerve fibers was lower in the TMD group (p<0.05), and they were negatively correlated with severity of the disease (p<0.05). Our study demonstrated that the density of corneal epithelial cells, stromal cells, and sensory plexus nerve fibers was significantly reduced in the TMD group. The pathological changes were more obvious in the marginal cornea, and it is correlated with severity of the disease.

scholarly journals Effect of diagnostic ultrasound on corneal apoptosis in rats

2020 ◽  
Vol 19 (9) ◽  
pp. 1947-1951
Author(s):  
Cong Liu ◽  
Xin Yu ◽  
Yun Kou
Keyword(s):  
Endothelial Cells ◽  
Epithelial Cells ◽  
Stromal Cells ◽  
Irradiation Time ◽  
Diagnostic Ultrasound ◽  
Male Rats ◽  
Control Group ◽  
Corneal Epithelial Cells ◽  
Corneal Epithelial ◽  
Apoptosis Rate

Purpose: To investigate the effect of diagnostic ultrasound on corneal apoptosis in rats.Methods: 24 male rats were randomly divided into 4 groups: control group, 10, 20 and 30 min group. The eyeballs of rats were irradiated  continuously for different time lengths by Siemens ACUSON S2000 color Doppler ultrasound diagnostic instrument. 24 hours later, the animals were killed and the corneas were taken for Tunel apoptosis detection. The apoptosis rates of corneal epithelial cells, stromal cells and endothelial cells were calculated.Results: Apoptotic cells were detected in corneal epithelial cells, stromal cells and endothelial cells of normal rats. There was no significant difference between the 10 min group and the control group (P>0.05). The apoptosis rate of 20 min and 30 min groups was significantly higher than that of the control group. With the extension of irradiation time, the apoptosis rate of corneal epithelial cells, stromal cells and endothelial cells increased.Conclusion: 20 min of rat eyeball irradiated by diagnostic ultrasound can increase the apoptosis of corneal cells, and the apoptosis is aggravated with the prolongation of ultrasound irradiation time. Keywords: Cornea; Ultrasonography; Apoptosis; Epithelial cells

scholarly journals Trehalose-Based Eye Drops Preserve Viability and Functionality of Cultured Human Corneal Epithelial Cells during Desiccation

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Aneta Hill-Bator ◽  
Marta Misiuk-Hojło ◽  
Krzysztof Marycz ◽  
Jakub Grzesiak
Keyword(s):  
Epithelial Cells ◽  
Caspase 3 ◽  
Neutral Red ◽  
Immunofluorescent Staining ◽  
Eye Drops ◽  
Fluorescent Assay ◽  
Corneal Epithelial Cells ◽  
Corneal Epithelial ◽  
Human Corneal Epithelial Cells ◽  
Active Caspase

This paper presents the evaluation of cytoprotective ability of trehalose-based eye drops in comparison with commercially available preparations during the experimental desiccation of cultured human corneal epithelial cells. Cultured human corneal epithelial cells (hCEC) underwent incubation with 7 different, commercially available medicaments used commonly in dry eye syndrome treatment, followed by desiccation trial performed on air under the flow hood for 5, 15, 30, and 45 minutes. Cell viability was quantified by live/dead fluorescent assay, while the presence of apoptotic cells was estimated by immunofluorescent staining for active caspase 3 protein. The preservation of membrane functions was evaluated using neutral red staining, while the preservation of proper morphology and phenotype was determined by fluorescent staining for actin filaments, nuclei, and p63 protein. The trehalose-based eye drops showed the highest efficiency in prevention of cell death from desiccation; moreover, this preparation preserved the normal cellular morphology, functions of cell membrane, and proliferative activity more effectively than other tested medicaments.

Stromal cell and human prostatic epithelial cell in-vitro co-coltures: Growth and morphology

1996 ◽  
Vol 63 (1_suppl) ◽  
pp. 65-68
Author(s):  
S. De Angeli ◽  
A. Fandella ◽  
C. Gatto ◽  
S. Buoro ◽  
C. Favretti ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Stromal Cells ◽  
Marked Reduction ◽  
Stromal Cell ◽  
Optical Microscope ◽  
Neutral Red ◽  
Cellular Growth ◽  
Murine Fibroblasts ◽  
Prostatic Epithelial Cell

A study was carried out on the effect of stroma-epithelium interaction on cellular growth and morphology in co-coltures of U285 prostatic epithelial cells with human prostatic and esophageal stromal cells and with murine fibroblasts of the 3T3-J2 line. The proliferation rate was determined by growth tests of neutral red and kenacid blue. Morphological observations were made under optical microscope on the same cultures used for the growth tests. Results highlighted a marked reduction in cellular growth in the co-cultures compared to control cultures, as well as the tendency of the stromal and epithelial cells to re-organise themselves in pseudo-acinous structures.

Carboxymethyl β-glucan Binds to Corneal Epithelial Cells and Increases Cell Adhesion to Laminin and Resistance to Oxidative Stress

Cornea ◽  
2007 ◽  
Vol 26 (1) ◽  
pp. 73-79 ◽  
Author(s):  
Marco Porcu ◽  
Francesco Guarna ◽  
Laura Formentini ◽  
Giuseppe Faraco ◽  
Silvia Fossati ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cell Adhesion ◽  
Epithelial Cells ◽  
Corneal Epithelial Cells ◽  
Corneal Epithelial
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