scholarly journals Development of a novel recombinant cell line for detection and characterization of Ah receptor nuclear translocation in intact cells

2020 ◽  
Vol 66 ◽  
pp. 104873 ◽  
Author(s):  
Ai Hayashi ◽  
Michael S. Denison
Keyword(s):  
Cell Line ◽  
Nuclear Translocation ◽  
Ah Receptor ◽  
Intact Cells ◽  
Recombinant Cell Line ◽  
Recombinant Cell

Identification of Classifiers for Increase or Decrease of Thyroid Peroxidase Activity in the FTC-238/hTPO Recombinant Cell Line

2011 ◽  
Vol 45 (18) ◽  
pp. 7906-7914 ◽  
Author(s):  
Mee Song ◽  
Youn-Jung Kim ◽  
Mi-Kyung Song ◽  
Han-Seam Choi ◽  
Yong-Keun Park ◽  
...  
Keyword(s):  
Cell Line ◽  
Peroxidase Activity ◽  
Thyroid Peroxidase ◽  
Recombinant Cell Line ◽  
Recombinant Cell

Recombinant Cell Line

2010 ◽  
pp. 1142-1142
Author(s):  
Stan Floresco ◽  
Robert Kessler ◽  
Ronald L. Cowan ◽  
Robert Kessler ◽  
Ronald L. Cowan ◽  
...  
Keyword(s):  
Cell Line ◽  
Recombinant Cell Line ◽  
Recombinant Cell

Evaluation of androgenic bioactivity in human serum by recombinant cell line: preliminary results

2002 ◽  
Vol 198 (1-2) ◽  
pp. 123-129 ◽  
Author(s):  
Françoise Paris ◽  
Nadège Servant ◽  
Béatrice Térouanne ◽  
Charles Sultan
Keyword(s):  
Human Serum ◽  
Cell Line ◽  
Preliminary Results ◽  
Recombinant Cell Line ◽  
Recombinant Cell

scholarly journals Development of Recombinant Cell Line Co-expressing Mutated Nav1.5, Kir2.1, and hERG for the Safety Assay of Drug Candidates

2012 ◽  
Vol 17 (6) ◽  
pp. 773-784 ◽  
Author(s):  
Masato Fujii ◽  
Susumu Ohya ◽  
Hisao Yamamura ◽  
Yuji Imaizumi
Keyword(s):  
Cell Death ◽  
Cell Line ◽  
Gene Transfection ◽  
Screening Method ◽  
Hek293 Cells ◽  
Wild Type ◽  
Single Action ◽  
Channel Inhibition ◽  
Recombinant Cell Line ◽  
Recombinant Cell

To provide a high-throughput screening method for human ether-a-go-go-gene–related gene (hERG) K+ channel inhibition, a new recombinant cell line, in which single action potential (AP)–induced cell death was produced by gene transfection. Mutated human cardiac Na+ channel Nav1.5 (IFM/Q3), which shows extremely slow inactivation, and wild-type inward rectifier K+ channel, Kir2.1, were stably co-expressed in HEK293 cells (IFM/Q3+Kir2.1). In IFM/Q3+Kir2.1, application of single electrical stimulation (ES) elicited a long AP lasting more than 30 s and led cells to die by more than 70%, whereas HEK293 co-transfected with wild-type Nav1.5 and Kir2.1 fully survived. The additional expression of hERG K+ channels in IFM/Q3+Kir2.1 shortened the duration of evoked AP and thereby markedly reduced the cell death. The treatment of the cells with hERG channel inhibitors such as nifekalant, E-4031, cisapride, terfenadine, and verapamil, recovered the prolonged AP and dose-dependently facilitated cell death upon ES. The EC50 values to induce the cell death were 3 µM, 19 nM, 17 nM, 74 nM, and 3 µM, respectively, whereas 10 µM nifedipine did not induce cell death. Results indicate the high utility of this cell system for hERG K+ channel safety assay.

scholarly journals Accelerating stable recombinant cell line development by targeted integration

2013 ◽  
Vol 7 (S6) ◽  
Author(s):  
Bernd Rehberger ◽  
Claas Wodarczyk ◽  
Britta Reichenbächer ◽  
Janet Köhler ◽  
Renée Weber ◽  
...  
Keyword(s):  
Cell Line ◽  
Cell Line Development ◽  
Recombinant Cell Line ◽  
Targeted Integration ◽  
Recombinant Cell
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