Freshly isolated primary human proximal tubule cells as an in vitro model for the detection of renal tubular toxicity

Toxicology ◽  
2020 ◽  
Vol 442 ◽  
pp. 152535 ◽  
Author(s):  
Piyush Bajaj ◽  
Git Chung ◽  
Keith Pye ◽  
Tomoya Yukawa ◽  
Akio Imanishi ◽  
...  
Keyword(s):  
Proximal Tubule ◽  
In Vitro Model ◽  
Proximal Tubule Cells ◽  
Tubular Toxicity ◽  
Tubule Cells ◽  
Vitro Model ◽  
Renal Tubular ◽  
Human Proximal Tubule

Caki-1 Cells Represent an in vitro Model System for Studying the Human Proximal Tubule Epithelium

2007 ◽  
Vol 107 (2) ◽  
pp. e47-e56 ◽  
Author(s):  
Natalie Glube ◽  
Andreas Giessl ◽  
Uwe Wolfrum ◽  
Peter Langguth
Keyword(s):  
Proximal Tubule ◽  
In Vitro Model ◽  
Model System ◽  
In Vitro Model System ◽  
Vitro Model ◽  
Human Proximal Tubule

Clusterin protects renal tubular epithelial cells from gentamicin-mediated cytotoxicity

2002 ◽  
Vol 282 (4) ◽  
pp. F703-F709 ◽  
Author(s):  
Richard A. Girton ◽  
David P. Sundin ◽  
Mark E. Rosenberg
Keyword(s):  
Proximal Tubule ◽  
Cell Injury ◽  
Renal Tubular Cell ◽  
Dependent Manner ◽  
Proximal Tubule Cells ◽  
Lactate Dehydrogenase Release ◽  
Tubule Cells ◽  
Renal Proximal Tubule Cells ◽  
Renal Tubular

Clusterin is a heterodimeric secreted glycoprotein that is upregulated after acute renal injury. In aminoglycoside nephrotoxicity, clusterin is induced in the tubular epithelium and increased levels are found in the urine. In this study, we developed an in vitro model of gentamicin-induced cytotoxicity in renal proximal tubule cells and tested whether clusterin protected these cells from injury. LLC-PK1 cells were incubated with varying concentrations of gentamicin in serum-free media, and cytotoxicity was quantified by lactate dehydrogenase release and confirmed by vital dye exclusion. A dose-dependent increase in cytotoxicity occurred with gentamicin concentrations up to 27 mg/ml. Clusterin decreased cytotoxicity in a dose- and time-dependent manner at 6, 12, and 24 h, whereas albumin, used as a control protein, had no effect. In contrast to the aminoglycoside model, when cells were injured by depletion of ATP, clusterin had only a minimally protective effect. LLC-PK1 cells did not express megalin, a receptor that can mediate the uptake of both clusterin and aminoglycosides into proximal tubule cells. Uptake of gentamicin into LLC-PK1cells was observed despite the absence of megalin. In conclusion, clusterin specifically protects against gentamicin-induced renal tubular cell injury by a megalin-independent mechanism.

Molecular Size and Origin Do Not Influence the Harmful Side Effects of Hydroxyethyl Starch on Human Proximal Tubule Cells (HK-2) In Vitro

2014 ◽  
Vol 119 (3) ◽  
pp. 570-577 ◽  
Author(s):  
Raphael R. Bruno ◽  
Winfried Neuhaus ◽  
Norbert Roewer ◽  
Christian Wunder ◽  
Martin A. Schick
Keyword(s):  
Side Effects ◽  
Proximal Tubule ◽  
Hydroxyethyl Starch ◽  
Molecular Size ◽  
Proximal Tubule Cells ◽  
Tubule Cells ◽  
Human Proximal Tubule

scholarly journals SP781THE EFFECT OF IN VITRO TACROLIMUS EXPOSURE AND PHARMACOGENETIC VARIATION ON MULTILEVEL CYP3A5, ABCB1 EXPRESSION AND CTGF PRODUCTION IN HUMAN PROXIMAL TUBULE CELLS

2017 ◽  
Vol 32 (suppl_3) ◽  
pp. iii407-iii407
Author(s):  
Noel Knops ◽  
Yasaman Ramazani ◽  
Bert van den Heuvel ◽  
Elena Levtchenko ◽  
Dirk Kuypers
Keyword(s):  
Proximal Tubule ◽  
Proximal Tubule Cells ◽  
Tubule Cells ◽  
Human Proximal Tubule

scholarly journals Comparison of diglycolic acid exposure to human proximal tubule cells in vitro and rat kidneys in vivo

2017 ◽  
Vol 4 ◽  
pp. 342-347 ◽  
Author(s):  
Miriam E. Mossoba ◽  
Sanah Vohra ◽  
Howard Toomer ◽  
Shelia Pugh-Bishop ◽  
Zachary Keltner ◽  
...  
Keyword(s):  
Proximal Tubule ◽  
Acid Exposure ◽  
Proximal Tubule Cells ◽  
Diglycolic Acid ◽  
Tubule Cells ◽  
Human Proximal Tubule ◽  
Rat Kidneys
Sign in / Sign up

Export Citation Format

Share Document