Characterization of a novel inhibitor of coagulation factor XIa identified from salivary gland transcripts of the lone star tick, Amblyomma americanum

Toxicon ◽  
2020 ◽  
Vol 177 ◽  
pp. S44
Author(s):  
Mrinalini ◽  
Cho Yeow Koh ◽  
Aaron Wei Liang Li ◽  
Rohan Bendre ◽  
Mark Yan Yee Chan ◽  
...  
Keyword(s):  
Salivary Gland ◽  
Coagulation Factor ◽  
Amblyomma Americanum ◽  
Lone Star Tick ◽  
Factor Xia

Effect of methoprene and 20-hydroxyecdysone on salivary gland development of the lone star tick, Amblyomma americanum (L.)

1989 ◽  
Vol 35 (4) ◽  
pp. 313-320 ◽  
Author(s):  
Kent S. Shelby ◽  
Katherine M. Kocan ◽  
John A. Bantle ◽  
John R. Sauer
Keyword(s):  
Salivary Gland ◽  
Amblyomma Americanum ◽  
Lone Star Tick ◽  
Gland Development

scholarly journals Characterization of Novel Forms of Coagulation Factor XIa

2008 ◽  
Vol 283 (11) ◽  
pp. 6696-6705 ◽  
Author(s):  
Stephen B. Smith ◽  
Ingrid M. Verhamme ◽  
Mao-fu Sun ◽  
Paul E. Bock ◽  
David Gailani
Keyword(s):  
Coagulation Factor ◽  
Factor Xia

Selection and Characterization of a DNA Aptamer Inhibiting Coagulation Factor XIa

2016 ◽  
Vol 30 (3) ◽  
pp. 146-147
Author(s):  
David A. Donkor ◽  
Varsha Bhakta ◽  
William P. Sheffield
Keyword(s):  
Coagulation Factor ◽  
Dna Aptamer ◽  
Factor Xia

scholarly journals Selection and characterization of a DNA aptamer inhibiting coagulation factor XIa

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
David A. Donkor ◽  
Varsha Bhakta ◽  
Louise J. Eltringham-Smith ◽  
Alan R. Stafford ◽  
Jeffrey I. Weitz ◽  
...  
Keyword(s):  
Coagulation Factor ◽  
Dna Aptamer ◽  
Factor Xia

Isolation and Structural Charaderization of a Potent Inhibitor of Coagulation Factor Xa from the Leech Haementeria ghilianii

1989 ◽  
Vol 61 (03) ◽  
pp. 437-441 ◽  
Author(s):  
Cindra Condra ◽  
Elka Nutt ◽  
Christopher J Petroski ◽  
Ellen Simpson ◽  
P A Friedman ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Salivary Gland ◽  
Amino Acid ◽  
Amino Acid Sequence ◽  
Western Blot ◽  
Potent Inhibitor ◽  
Factor Xa ◽  
Coagulation Factor ◽  
Sds Page ◽  
Bovine Factor

SummaryThe present work reports the discovery and charactenzation of an anticoagulant protein in the salivary gland of the giant bloodsucking leech, H. ghilianii, which is a specific and potent inhibitor of coagulation factor Xa. The inhibitor, purified to homogeneity, displayed subnanomolar inhibition of bovine factor Xa and had a molecular weight of approximately 15,000 as deduced by denaturing SDS-PAGE. The amino acid sequence of the first 43 residues of the H. ghilianii derived inhibitor displayed a striking homology to antistasin, the recently described subnanomolar inhibitor of factor Xa isolated from the Mexican leech, H. officinalis. Antisera prepared to antistasin cross-reacted with the H. ghilianii protein in Western Blot analysis. These data indicate that the giant Amazonian leech, H. ghilianii, and the smaller Mexican leech, H. officinalrs, have similar proteins which disrupt the normal hemostatic clotting mechanisms in their mammalian host’s blood.

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