An in vitro model of the renal proximal tubule composed of small intestinal submucosa (SIS) and human kidney-derived cells (hKDCs)

2013 ◽  
Vol 221 ◽  
pp. S140
Author(s):  
Anke Hoppensack ◽  
Johanna Schanz ◽  
Christian Kazanecki ◽  
David Colter ◽  
Heike Walles
Keyword(s):  
Proximal Tubule ◽  
In Vitro Model ◽  
Human Kidney ◽  
Renal Proximal Tubule ◽  
Small Intestinal Submucosa ◽  
Small Intestinal ◽  
Vitro Model ◽  
Intestinal Submucosa

LAT1 overexpression and function compensates downregulation of ASCT2 in an in vitro model of renal proximal tubule cell ageing

2010 ◽  
Vol 349 (1-2) ◽  
pp. 107-116 ◽  
Author(s):  
Maria João Pinho ◽  
José Miguel Cabral ◽  
Elisabete Silva ◽  
Maria Paula Serrão ◽  
Patrício Soares-da-Silva
Keyword(s):  
Proximal Tubule ◽  
In Vitro Model ◽  
Renal Proximal Tubule ◽  
Proximal Tubule Cell ◽  
Tubule Cell ◽  
Vitro Model ◽  
And Function

An in vitro model of renal proximal tubule cell regeneration

1993 ◽  
Vol 29 (4) ◽  
pp. 211-215 ◽  
Author(s):  
Sandra E. Kays ◽  
Carolyn D. Berdanier ◽  
Anne R. Swagler ◽  
Edward A. Lock ◽  
Rick G. Schnellmann
Keyword(s):  
Proximal Tubule ◽  
In Vitro Model ◽  
Renal Proximal Tubule ◽  
Cell Regeneration ◽  
Proximal Tubule Cell ◽  
Tubule Cell ◽  
Vitro Model

scholarly journals A Human In Vitro Model That Mimics the Renal Proximal Tubule

2014 ◽  
Vol 20 (7) ◽  
pp. 599-609 ◽  
Author(s):  
Anke Hoppensack ◽  
Christian C. Kazanecki ◽  
David Colter ◽  
Anna Gosiewska ◽  
Johanna Schanz ◽  
...  
Keyword(s):  
Proximal Tubule ◽  
In Vitro Model ◽  
Renal Proximal Tubule ◽  
Vitro Model

scholarly journals In vitro assessment of the starch digestibility of western Canadian wheat market classes and cultivars

2018 ◽  
Vol 98 (3) ◽  
pp. 463-476 ◽  
Author(s):  
Namalika D. Karunaratne ◽  
Dawn A. Abbott ◽  
Ravindra N. Chibbar ◽  
Pierre J. Hucl ◽  
Curtis J. Pozniak ◽  
...  
Keyword(s):  
Crude Protein ◽  
In Vitro Model ◽  
Starch Digestibility ◽  
Wheat Market ◽  
In Vitro Assessment ◽  
Market Class ◽  
Small Intestinal ◽  
Vitro Model ◽  
Total Starch

The objective of the study was to measure the effect of wheat market class and cultivar on starch digestibility using an in vitro model that mimics the chicken digestive tract and relate it to grain characteristics. The study evaluated 18 wheat cultivars from eight western Canadian wheat classes and, each cultivar was replicated four times. Samples were subjected to gastric and small intestine (SI) digestion phases and each sample was assayed in triplicate; glucose release was measured in SI phase. Starch granule distribution, amylose, total starch, crude protein (CP), ash, and non-starch polysaccharides (NSP) were analyzed in all wheat samples. Small intestinal phase times of 15, 60, and 120 min were chosen to approximate digestion in the terminal duodenum, jejunum, and ileum. Starch digestibility of wheat classes ranged as follows: 15 min — 33.1% to 49.1%, 60 min — 80.2% to 93.3%, and 120 min — 92.4% to 97.6%. Starch digestibility positively correlated with CP, ash, NSP, and proportion of large granules, whereas it negatively correlated with total starch, and proportion of small and medium granules. In conclusion, market class and cultivar of western Canadian wheat affects both rate and extent of starch digestibility and it is related to various grain characteristics.

scholarly journals Congener-Specific Polychlorinated Biphenyl–Induced Cell Death in Human Kidney Cells In Vitro: Potential Role of Caspase

2006 ◽  
Vol 25 (5) ◽  
pp. 341-347 ◽  
Author(s):  
Y. Q. Chen ◽  
S. De ◽  
S. Ghosh ◽  
S. K. Dutta
Keyword(s):  
Cell Death ◽  
Proximal Tubule ◽  
Human Kidney ◽  
Renal Proximal Tubule ◽  
Proximal Tubule Cell ◽  
Kidney Cells ◽  
Dependent Manner ◽  
Caspase Inhibitor ◽  
Pcb Congeners

Polychlorinated biphenyls (PCBs) are among the most widespread and persistent pollutants in the global environment. Coplanar and noncoplanar PCBs have been shown to cause congener-specific apoptosis mediated neurotoxicity in rats. Very few, if any, such studies have been reported on human renal cell toxicity. The authors report here caspase-dependent or caspase-independent renal toxicity, as measured by apoptotic death induced by PCBs, depending on the planarity of congeners PCB-77 (coplanar) and PCB-153 (noncoplanar) in human kidney cells (HK2) in vitro. The authors have combined morphological and biological techniques to discover the relevance of apoptosis in renal proximal tubule cell death induced by these two PCB congeners. Treatment with both PCB congeners caused accelerated apoptosis in a time-and concentration-dependent manner. Based on our findings using human kidney (HK2) cells, there was more apoptosis-mediated loss of cell viability by non– ortho-substituted PCB-77 when compared to PCB-153. A significant increase of caspase-3 expression through immunoblot studies showed the involvement of apoptosis by PCB-77 compared to none by PCB-153. The broad-spectrum caspase inhibitor z-VAD-fmk showed increased cell death when treated by PCB-153, but not by PCB-77, confirming that caspase inhibitor induced a switch in the mode of cell death. It is reasonable to assume that apoptotic cell death in the renal proximal tubule cells treated by PCBs may have both caspase-dependent and caspase-independent pathways.

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