Cellular and molecular evidence on the protection of embryonic stem cell-derived cardiomyocytes from phosphine-induced oxidative stress, mitochondrial dysfunction and apoptosis by mesenchymal stem cells derived microvesicles

2016 ◽  
Vol 258 ◽  
pp. S158
Author(s):  
M. Rezvanfar ◽  
M. Baeeri ◽  
Z. Afsartala ◽  
M. Hodjat ◽  
A. Baghaei ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Mitochondrial Dysfunction ◽  
Embryonic Stem ◽  
Molecular Evidence

scholarly journals Accelerated Wound Healing by Fibroblasts Differentiated from Human Embryonic Stem Cell-Derived Mesenchymal Stem Cells in a Pressure Ulcer Animal Model

2018 ◽  
Vol 2018 ◽  
pp. 1-12 ◽  
Author(s):  
Dajeong Yoon ◽  
Dogeon Yoon ◽  
Heejoong Sim ◽  
Inseok Hwang ◽  
Ji-Seon Lee ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Pressure Ulcer ◽  
Human Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Type I ◽  
Skin Ulcers

Fibroblasts synthesize and secrete dermal collagen, matrix proteins, growth factors, and cytokines. These characteristics of fibroblasts provide a potential way for fibroblast therapy to treat skin ulcers more effectively than conventional therapies such as cytokine therapy and negative pressure wound therapy. However, the obstacle to the commercialization of fibroblast therapy is the limited supply of cells with consistent quality. In this study, we tested whether human embryonic stem cell-derived mesenchymal stem cells (hESC-MSCs) could be differentiated into fibroblasts considering that they have characteristics of high differentiation rates, unlimited proliferation possibility from a single colony, and homogeneity. As a result, hESC-MSC-derived fibroblasts (hESC-MSC-Fbs) showed a significant increase in the expression of type I and III collagen, fibronectin, and fibroblast-specific protein-1 (FSP-1). Besides, vessel formation and wound healing were enhanced in hESC-MSC-Fb-treated skin tissues compared to PBS- or hESC-MSC-treated skin tissues, along with decreased IL-6 expression at 4 days after the formation of pressure ulcer wound in a mouse model. In view of the limited available cell sources for fibroblast therapy, hESC-MSC-Fbs show a promising potential as a commercial cell therapy source to treat skin ulcers.

scholarly journals Embryonic stem cell-derived extracellular vesicles enhance the therapeutic effect of mesenchymal stem cells

Theranostics ◽  
2019 ◽  
Vol 9 (23) ◽  
pp. 6976-6990 ◽  
Author(s):  
Yan Zhang ◽  
Jia Xu ◽  
Siying Liu ◽  
Meikuang Lim ◽  
Shuang Zhao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Therapeutic Effect ◽  
Extracellular Vesicles ◽  
Embryonic Stem

Human Embryonic Stem Cell-Derived Mesenchymal Stem Cells as Cellular Delivery Vehicles for Prodrug Gene Therapy of Glioblastoma

2011 ◽  
Vol 22 (11) ◽  
pp. 1365-1377 ◽  
Author(s):  
Xiao Ying Bak ◽  
Hoang Lam Dang ◽  
Jingye Yang ◽  
Kai Ye ◽  
Esther X.W. Lee ◽  
...  
Keyword(s):  
Stem Cells ◽  
Gene Therapy ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Human Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Cellular Delivery ◽  
Delivery Vehicles

Efficient Engineering of Vascularized Ectopic Bone from Human Embryonic Stem Cell–Derived Mesenchymal Stem Cells

2012 ◽  
Vol 18 (21-22) ◽  
pp. 2290-2302 ◽  
Author(s):  
Hagit Domev ◽  
Michal Amit ◽  
Ilana Laevsky ◽  
Ayelet Dar ◽  
Joseph Itskovitz-Eldor
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Human Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Ectopic Bone

scholarly journals Elucidating the Secretion Proteome of Human Embryonic Stem Cell-derived Mesenchymal Stem Cells

2007 ◽  
Vol 6 (10) ◽  
pp. 1680-1689 ◽  
Author(s):  
Siu Kwan Sze ◽  
Dominique P. V. de Kleijn ◽  
Ruenn Chai Lai ◽  
Eileen Khia Way Tan ◽  
Hui Zhao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Human Embryonic Stem Cell ◽  
Embryonic Stem

scholarly journals Asymptomatic neurotoxicity of amyloid β-peptides (Aβ1-42 and Aβ25-35) on mouse embryonic stem cell-derived neural cells

2020 ◽  
Author(s):  
Nur Izzati Mansor ◽  
Carolindah Makena Ntimi ◽  
Noraishah Mydin Abdul-Aziz ◽  
King-Hwa Ling ◽  
Aishah Adam ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Stem Cells ◽  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cell ◽  
Neural Cells ◽  
Aβ Peptides ◽  
Intracellular Ros

One of the strategies in the establishment of in vitro oxidative stress models for neurodegenerative diseases, such as Alzheimer’s disease (AD), is to induce neurotoxicity by amyloid beta (Aβ) peptides in suitable neural cells. Presently, data on the neurotoxicity of Aβ in neural cells differentiated from stem cells are limited. In this study, we attempted to induce oxidative stress in transgenic 46C mouse embryonic stem cell-derived neurons via treatment with Ab peptides (Aβ1-42 and Aβ25-35). 46C neural cells were generated by promoting the formation of multicellular aggregates, embryoid bodies (EBs) in the absence of leukemia inhibitory factor (LIF), followed by the addition of all-trans retinoic acid (ATRA) as the neural inducer. Mature neuronal cells were exposed to different concentrations of Aβ1-42 and Aβ25-35 for 24 h. Morphological changes, cell viability, and intracellular ROS production were assessed. We found that 100 µM Aβ1-42 and 50 µM Aβ25-35 only promoted 40% and 10%, respectively, of cell injury and death in the 46C-derived neuronal cells. Interestingly, treatment with each of the Aβ peptides resulted in a significant increase of intracellular ROS activity, as compared to untreated neurons. These findings indicate the potential of using neurons derived from stem cells and Aβ peptides in generating oxidative stress for the establishment of an in vitro AD model that could be useful for drug screening and natural product studies.

scholarly journals Embryonic Stem Cell-Derived Mesenchymal Stem Cells (MSCs) Have a Superior Neuroprotective Capacity Over Fetal MSCs in the Hypoxic-Ischemic Mouse Brain

2018 ◽  
Vol 7 (5) ◽  
pp. 439-449 ◽  
Author(s):  
Kate E. Hawkins ◽  
Michelangelo Corcelli ◽  
Kate Dowding ◽  
Anna M. Ranzoni ◽  
Filipa Vlahova ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Mouse Brain ◽  
Embryonic Stem
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