Recent advances in microdroplet techniques for single-cell protein analysis

2021 ◽  
pp. 116411
Author(s):  
Zhihang Yu ◽  
Jing Jin ◽  
Lingling Shui ◽  
Huaying Chen ◽  
Yonggang Zhu
Keyword(s):  
Single Cell ◽  
Protein Analysis ◽  
Single Cell Protein ◽  
Cell Protein ◽  
Recent Advances

Fluorescence imaging-based methods for single-cell protein analysis

2019 ◽  
Vol 411 (19) ◽  
pp. 4339-4347
Author(s):  
Siwen Wang ◽  
Fei Ji ◽  
Zhonghan Li ◽  
Min Xue
Keyword(s):  
Single Cell ◽  
Fluorescence Imaging ◽  
Protein Analysis ◽  
Single Cell Protein ◽  
Cell Protein

Scaling advantages and constraints in miniaturized capture assays for single cell protein analysis

Lab on a Chip ◽  
2013 ◽  
Vol 13 (11) ◽  
pp. 2066 ◽  
Author(s):  
Ali Salehi-Reyhani ◽  
Sanjiv Sharma ◽  
Edward Burgin ◽  
Michael Barclay ◽  
Anthony Cass ◽  
...  
Keyword(s):  
Single Cell ◽  
Protein Analysis ◽  
Single Cell Protein ◽  
Cell Protein

scholarly journals Recent advances in single cell protein use as a feed ingredient in aquaculture

2020 ◽  
Vol 61 ◽  
pp. 189-197 ◽  
Author(s):  
Shawn W Jones ◽  
Alon Karpol ◽  
Sivan Friedman ◽  
Biniam T Maru ◽  
Bryan P Tracy
Keyword(s):  
Single Cell ◽  
Single Cell Protein ◽  
Cell Protein ◽  
Feed Ingredient ◽  
Recent Advances

scholarly journals Microfluidic Platforms for Single-Cell Protein Analysis

2013 ◽  
Vol 18 (6) ◽  
pp. 446-454 ◽  
Author(s):  
Yanli Liu ◽  
Anup K. Singh
Keyword(s):  
Single Cell ◽  
Protein Analysis ◽  
Single Cell Protein ◽  
Cell Protein ◽  
Microfluidic Platforms

scholarly journals High-selectivity cytology via lab-on-a-disc western blotting of individual cells

Lab on a Chip ◽  
2017 ◽  
Vol 17 (5) ◽  
pp. 855-863 ◽  
Author(s):  
John J. Kim ◽  
Elly Sinkala ◽  
Amy E. Herr
Keyword(s):  
Single Cell ◽  
Western Blotting ◽  
High Selectivity ◽  
Protein Analysis ◽  
Single Cell Protein ◽  
Cell Protein ◽  
Cell Handling

We demonstrate a lab-on-a-disc western blotting device that integrates cell handling and single-cell protein analysis of sparse (<200 cells) starting samples.

scholarly journals Advances of Single-Cell Protein Analysis

Cells ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 1271 ◽  
Author(s):  
Lixing Liu ◽  
Deyong Chen ◽  
Junbo Wang ◽  
Jian Chen
Keyword(s):  
Flow Cytometry ◽  
Single Cell ◽  
Cell Biology ◽  
Protein Analysis ◽  
Single Cell Protein ◽  
Cell Protein ◽  
Future Research ◽  
Mass Cytometry ◽  
Tumor Treatment ◽  
Protein Expressions

Proteins play a significant role in the key activities of cells. Single-cell protein analysis provides crucial insights in studying cellular heterogeneities. However, the low abundance and enormous complexity of the proteome posit challenges in analyzing protein expressions at the single-cell level. This review summarizes recent advances of various approaches to single-cell protein analysis. We begin by discussing conventional characterization approaches, including fluorescence flow cytometry, mass cytometry, enzyme-linked immunospot assay, and capillary electrophoresis. We then detail the landmark advances of microfluidic approaches for analyzing single-cell protein expressions, including microfluidic fluorescent flow cytometry, droplet-based microfluidics, microwell-based assay (microengraving), microchamber-based assay (barcoding microchips), and single-cell Western blotting, among which the advantages and limitations are compared. Looking forward, we discuss future research opportunities and challenges for multiplexity, analyte, throughput, and sensitivity of the microfluidic approaches, which we believe will prompt the research of single-cell proteins such as the molecular mechanism of cell biology, as well as the clinical applications for tumor treatment and drug development.

Single-cell protein analysis of a single mouse embryo by two-dimensional capillary electrophoresis

2006 ◽  
Vol 1130 (2) ◽  
pp. 190-194 ◽  
Author(s):  
Melissa M. Harwood ◽  
Elisabeth S. Christians ◽  
Md. Abul Fazal ◽  
Norman J. Dovichi
Keyword(s):  
Capillary Electrophoresis ◽  
Single Cell ◽  
Mouse Embryo ◽  
Protein Analysis ◽  
Single Cell Protein ◽  
Cell Protein ◽  
Two Dimensional
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