feed ingredient
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2022 ◽  
Vol 4 (1) ◽  
Author(s):  
Yanxian Li ◽  
Karina Gajardo ◽  
Alexander Jaramillo-Torres ◽  
Trond M. Kortner ◽  
Åshild Krogdahl

Abstract Background Being part of fish's natural diets, insects have become a practical alternative feed ingredient for aquaculture. While nutritional values of insects have been extensively studied in various fish species, their impact on the fish microbiota remains to be fully explored. In an 8-week freshwater feeding trial, Atlantic salmon (Salmo salar) were fed either a commercially relevant reference diet or an insect meal diet wherein black soldier fly (Hermetia illucens) larvae meal comprised 60% of total ingredients. Microbiota of digesta and mucosa origin from the proximal and distal intestine were collected and profiled along with feed and water samples. Results The insect meal diet markedly modulated the salmon intestinal microbiota. Salmon fed the insect meal diet showed similar or lower alpha-diversity indices in the digesta but higher alpha-diversity indices in the mucosa. A group of bacterial genera, dominated by members of the Bacillaceae family, was enriched in salmon fed the insect meal diet, which confirms our previous findings in a seawater feeding trial. We also found that microbiota in the intestine closely resembled that of the feeds but was distinct from the water microbiota. Notably, bacterial genera associated with the diet effects were also present in the feeds. Conclusions We conclude that salmon fed the insect meal diets show consistent changes in the intestinal microbiota. The next challenge is to evaluate the extent to which these alterations are attributable to feed microbiota and dietary nutrients, and what these changes mean for fish physiology and health.


2022 ◽  
Author(s):  
Dewi Apri Astuti ◽  
Komang Gede Wiryawan

2022 ◽  
Author(s):  
Usman Elahi ◽  
Changchun Xu ◽  
Jing Wang ◽  
Jing Lin ◽  
Shugeng Wu ◽  
...  
Keyword(s):  

Author(s):  
Keelee J McCarty ◽  
Jessie E Tipton ◽  
Ralph E Ricks ◽  
Jessica Danielo ◽  
Jesse S Thompson ◽  
...  

Abstract The objective was to determine the effects of an immunomodulatory feed ingredient following weaning on cytokine expression and fecal microbial populations of heifers. Commercial Angus heifers (n = 72) were weaned (227 ± 7 d of age), blocked by BW (n = 9 blocks) and randomly assigned to one of 2 pens per block. Pens within weight block (4 heifers/pen) were then randomly assigned to treatments. Heifers were fed twice daily from d 0-60 (to gain 0.75kg/day) and top-dressed with either 18g/heifer/d of the immunomodulatory feed ingredient (Celmanax; Arm and Hammer Animal Nutrition, Princeton, NJ, USA; CEL) or corn-germ meal (CON). Blood samples were collected on d 0, 15, 30, 45, 60 and fecal grab samples on d 0 of the feeding trial. After d 60, two heifers per pen (n=32) were randomly selected for a transportation challenge. Serum samples were collected at h 0, 4, 8, 12 and fecal grab samples at h -24, 0, 24 and 7d post-challenge. Blood samples were analyzed for interferonγ (IFNγ), interleukin-8 (IL-8), and haptoglobin (HP) using commercially available ELISA kits and qRT-PCR for genes of interest associated with cytokine expression. Fecal samples were enumerated for Clostridia and E. coli using selective media (≤ 5 isolates from each media/sample), tested to determine if they were C. perfringens or pathogenic E. coli, and then enriched for detection of Salmonella. Data was analyzed via ANOVA. During the feeding trial, HP was reduced (P = 0.018) in CEL compared to CON at d 15, 45, and 60, while IFNγ and IL-8 did not differ (P > 0.080) between treatments. All cytokines were decreased (P < 0.001) in CEL compared to CON during the challenge. During the feeding trial, HP mRNA was increased (P = 0.045) in CEL compared to CON at d 30 and 60. Similarly, IFNγ mRNA was increased (P = 0.040) in CEL compared to CON, however, other genes of interest did not differ (P > 0.172). Both C. perfringens and total E. coli counts were decreased (P = 0.036) in CEL compared to CON at 24h after the start of the transportation challenge. Clostridia and pathogenic E. coli counts did not differ (P = 0.941) between treatments. Total Clostridia and E. coli counts were increased (P < 0.014) 24h post-challenge. All microbial populations, except pathogenic E. coli, observed decreased (P ≤ 0.009) counts from 24h to 7d post-challenge. Overall, Celmanax supplementation decreased circulating cytokines, and altered microbial populations and gene expression, thus, may serve a role in preparing animals to better cope with immunological challenges.


2021 ◽  
Vol 54 (1) ◽  
Author(s):  
Sutisa Khempaka ◽  
Chayanan Pukkung ◽  
Supattra Okrathok ◽  
Siriporn Chaiyasit ◽  
Arporn Khimkem ◽  
...  

2021 ◽  
Vol 2 (2) ◽  
pp. 260-275
Author(s):  
Ademola Adebukola ADENIGBA ◽  
Samuel Dare OLUWAGBAYIDE

This study was conducted to evaluate the effect of rotor speed and screen size on power consumed during milling operation. The milling system was tested using three fish feed ingredients; bone meal, groundnut cake and maize. The moisture contents of the ingredients bought from the market are 13.1%, 14.7% and 17.5% dry basis, respectively. The milling machine was evaluated with the 3 kg of each feed ingredient and was replicated three times for each of the experimental parameters. The machine parameters varied during the experiment includes four screen sizes (1.5 mm, 2.0 mm, 2.5 mm and 3.0 mm) and five rotor speeds (1500 rpm, 1800 rpm, 2100 rpm, 2400 rpm and 2700 rpm). Regression analysis was carried out on the data collated. The analysis was used to develop a model which is capable of predicting the electrical energy (kJ) consumed. There was no significant effect of screen size on the average power consumed during milling since there is no linear relationship between power consumed and screen size. However, there is a significant effect of speed on average power consumed, the power consumed increases as speed decreases therefore making milling operation at higher speed to be cost effective since it doesn’t require much power to achieve the required output. The P-Value depicts that screen size has no significant effect on the electrical energy consumed during the milling operation while speed has a significant effect on the electrical energy used at 95% confidence level.


Agriculture ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 1176
Author(s):  
Kari L. Harding ◽  
Thien Vu ◽  
Rebecca Wysocky ◽  
Ramon Malheiros ◽  
Kenneth E. Anderson ◽  
...  

The abundance of peanut and poultry production within the state of North Carolina and the US Southeast, led us to conduct a layer feeding trial to determine the utilization of whole-in-shell high-oleic peanuts (WPN) and/or unblanched high-oleic peanuts (HOPN) as an alternative feed ingredient for poultry. To meet this objective, we randomly assigned 576 shaver hens to 4 dietary treatments (4 rep/trt). The dietary treatments consisted of a conventional control diet (C1), a diet containing 4% WPN, an 8% HOPN diet, and a control diet containing soy protein isolate (C2). Feed and water were provided for 6 weeks ad libitum. Pen body weights (BW) were recorded at week 0 and week 6 (wk6), and feed weights were recorded bi-weekly. Shell eggs were collected daily and enumerated. Bi-weekly 120 eggs/treatment were collected for quality assessment and egg weight (EW), while 16 eggs/treatment were collected for chemical analysis. There were no significant differences in BW or EW at week 6. Hens fed the C2 produced more total dozen eggs relative to C1 hens over the feeding trial (p < 0.05). Hens fed the C1 diet consumed less total feed relative to the other treatments with the best feed conversion ratio (p < 0.05). Most eggs produced from each treatment were USDA grade A, large eggs. There were no differences in egg quality, with the exception of yolk color, with significantly higher yolk color scores in eggs produced from the C1 and C2 treatments relative to the other treatments (p < 0.05). Eggs produced from the HOPN treatment had significantly reduced stearic and linoleic fatty acid levels relative to the other treatments (p < 0.05). Eggs produced from hens fed the WPN diet had significantly greater β-carotene content relative to eggs from the other treatment groups (p < 0.05). In summary, this study suggests that WPN and/or HOPN may be a suitable alternative layer feed ingredient and a dietary means to enrich the eggs produced while not adversely affecting hen performance.


Foods ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2744
Author(s):  
Runa Thrastardottir ◽  
Hildur Thora Olafsdottir ◽  
Ragnheidur Inga Thorarinsdottir

Insects are part of the diet of over 2 billion people worldwide; however, insects have not been popular in Europe, neither as food nor as a feed ingredient. This has been changing in recent years, due to increased knowledge regarding the nutritional benefits, the need for novel protein production and the low environmental impact of insects compared to conventional protein production. The purpose of this study is to give an overview of the most popular insects farmed in Europe, yellow mealworm, Tenebrio molitor, and black soldier fly (BSF), Hermetia illucens, together with the main obstacles and risks. A comprehensive literature study was carried out and 27 insect farming companies found listed in Europe were contacted directly. The results show that the insect farming industry is increasing in Europe, and the success of the frontrunners is based on large investments in technology, automation and economy of scale. The interest of venture capital firms is noticeable, covering 90% of the investment costs in some cases. It is concluded that insect farming in Europe is likely to expand rapidly in the coming years, offering new proteins and other valuable products, not only as a feed ingredient, but also for human consumption. European regulations have additionally been rapidly changing, with more freedom towards insects as food and feed. There is an increased knowledge regarding safety concerns of edible insects, and the results indicate that edible insects pose a smaller risk for zoonotic diseases than livestock. However, knowledge regarding risk posed by edible insects is still lacking, but food and feed safety is essential to put products on the European market.


2021 ◽  
Author(s):  
Rakhi Bajpai Dixit ◽  
Uma Shankar Sagaram ◽  
Chandra Gocher ◽  
G. Raja Krishna Kumar ◽  
Santanu Dasgupta

Author(s):  
Yuzer Alfiko ◽  
Dizhi Xie ◽  
Retno Tri Astuti ◽  
Joey Wong ◽  
Le Wang

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