Specificity of 136 patient’s antibodies to human red blood cells in Dr. Max Peralta J Hospital Blood Bank 2004–February 2009

2010 ◽  
Vol 42 (2) ◽  
pp. 105-108 ◽  
Author(s):  
César Cerdas-Quesada
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Blood Bank ◽  
Human Red Blood Cells

scholarly journals Methylation of protein aspartates and deamidated asparagines as a function of blood bank storage and oxidative stress in human red blood cells

Transfusion ◽  
2018 ◽  
Vol 58 (12) ◽  
pp. 2978-2991 ◽  
Author(s):  
Julie A. Reisz ◽  
Travis Nemkov ◽  
Monika Dzieciatkowska ◽  
Rachel Culp-Hill ◽  
Davide Stefanoni ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Blood Bank ◽  
Human Red Blood Cells ◽  
And Oxidative Stress

scholarly journals Dielectrophoretic Detection of Electrical Property Changes of Stored Human Red Blood Cells

2021 ◽  
Author(s):  
Edwin David Lavi
Keyword(s):  
Electrical Property ◽  
Electrical Properties ◽  
Red Blood Cells ◽  
Human Blood ◽  
Blood Cells ◽  
Blood Bank ◽  
Crosslinking Reaction ◽  
Ion Leakage ◽  
Human Red Blood Cells ◽  
Property Changes

The ability to transport and store a large human blood inventory for transfusions is an essential requirement for medical institutions. Thus, there is an important need for rapid and low-cost characterization tools for analyzing the properties of human red blood cells (RBCs) while in storage. In this study, we investigate the ability to use dielectrophoresis (DEP) for measuring the storage-induced changes in RBC electrical properties. Fresh human blood was collected, suspended in K2-EDTA anticoagulant and stored in a blood bank refrigerator for a period of 20 days. Cells were removed from storage at 5-day intervals and subjected to a glutaraldehyde crosslinking reaction to “freeze” cells at their ionic equilibrium at that point in time and prevent ion leakage during DEP analysis. The DEP behavior of RBCs was analyzed in a high permittivity DEP buffer using a three-dimensional DEP chip (3DEP) and also compared to measurements taken with a 2D quadrupole electrode array. The DEP analysis confirms that RBC electrical property changes occur during storage and are only discernable with the use of the cell crosslinking reaction above a glutaraldehyde fixation concentration of 1.0 w/v%. In particular, cytoplasm conductivity was observed to decrease by more than 75% while the RBC membrane conductance was observed to increase by more than 1000% over a period of 20 days. These results show that the presented combination of chemical crosslinking and DEP can be used as rapid characterization tool for monitoring electrical properties changes of human red blood cells while subjected to refrigeration in blood bank storage.

scholarly journals Transbilayer distribution and mobility of phosphatidylinositol in human red blood cells.

1990 ◽  
Vol 265 (27) ◽  
pp. 16035-16038 ◽  
Author(s):  
P Bütikofer ◽  
Z W Lin ◽  
D T Chiu ◽  
B Lubin ◽  
F A Kuypers
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Human Red Blood Cells

scholarly journals Rhythmic glucose metabolism regulates the redox circadian clockwork in human red blood cells

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Ratnasekhar Ch ◽  
Guillaume Rey ◽  
Sandipan Ray ◽  
Pawan K. Jha ◽  
Paul C. Driscoll ◽  
...  
Keyword(s):  
Glucose Metabolism ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Mammalian Cells ◽  
Metabolic Flux ◽  
Pentose Phosphate ◽  
Human Red Blood Cells ◽  
Integral Process ◽  
Metabolic Oscillations ◽  
Human Rbcs

AbstractCircadian clocks coordinate mammalian behavior and physiology enabling organisms to anticipate 24-hour cycles. Transcription-translation feedback loops are thought to drive these clocks in most of mammalian cells. However, red blood cells (RBCs), which do not contain a nucleus, and cannot perform transcription or translation, nonetheless exhibit circadian redox rhythms. Here we show human RBCs display circadian regulation of glucose metabolism, which is required to sustain daily redox oscillations. We found daily rhythms of metabolite levels and flux through glycolysis and the pentose phosphate pathway (PPP). We show that inhibition of critical enzymes in either pathway abolished 24-hour rhythms in metabolic flux and redox oscillations, and determined that metabolic oscillations are necessary for redox rhythmicity. Furthermore, metabolic flux rhythms also occur in nucleated cells, and persist when the core transcriptional circadian clockwork is absent in Bmal1 knockouts. Thus, we propose that rhythmic glucose metabolism is an integral process in circadian rhythms.

Sign in / Sign up

Export Citation Format

Share Document