Molecular detection and identification of hemoparasites in pampas deer (Ozotoceros bezoarticus Linnaeus, 1758) from the Pantanal Brazil

2013 ◽  
Vol 4 (4) ◽  
pp. 341-345 ◽  
Author(s):  
Júlia A.G. Silveira ◽  
Élida M.L. Rabelo ◽  
Ana C.R. Lacerda ◽  
Paulo A.L. Borges ◽  
Walfrido M. Tomás ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Ozotoceros Bezoarticus ◽  
Detection And Identification

First molecular detection and identification of Trypanosoma evansi in goats from Cebu, Philippines using a PCR-based assay

2020 ◽  
Vol 21 ◽  
pp. 100414
Author(s):  
Afraa Elata ◽  
Eloiza May Galon ◽  
Paul Franck Adjou Moumouni ◽  
Rochelle Haidee D. Ybanez ◽  
Ehab Mossaad ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Trypanosoma Evansi ◽  
Detection And Identification

scholarly journals Molecular detection and identification of Rickettsia endosymbiont in different biotypes of Bemisia tabaci

2009 ◽  
Vol 15 ◽  
pp. 271-272 ◽  
Author(s):  
G. Gueguen ◽  
J.M. Rolain ◽  
E. Zchori-Fein ◽  
F. Vavre ◽  
F. Fleury ◽  
...  
Keyword(s):  
Bemisia Tabaci ◽  
Molecular Detection ◽  
Detection And Identification

scholarly journals Molecular Detection and Identification of Rickettsia Species in Ixodes pacificus in California

2011 ◽  
Vol 11 (7) ◽  
pp. 957-961 ◽  
Author(s):  
Jimmy Ninh Phan ◽  
Casey Roy Lu ◽  
William Garrett Bender ◽  
Robert Marion Smoak ◽  
Jianmin Zhong
Keyword(s):  
Molecular Detection ◽  
Ixodes Pacificus ◽  
Rickettsia Species ◽  
Detection And Identification

Moving towards an integrated approach to molecular detection and identification ofToxoplasma gondii

Parasitology ◽  
2009 ◽  
Vol 137 (1) ◽  
pp. 1-11 ◽  
Author(s):  
C. SU ◽  
E. K. SHWAB ◽  
P. ZHOU ◽  
X. Q. ZHU ◽  
J. P. DUBEY
Keyword(s):  
Molecular Detection ◽  
Genetic Characterization ◽  
Integrated Approach ◽  
Epidemiological Studies ◽  
Molecular Methods ◽  
Maximum Information ◽  
The Past ◽  
Phylogenetic Studies ◽  
Detection And Identification

SUMMARYThe development of simple, sensitive and rapid methods for the detection and identification ofToxoplasma gondiiis important for the diagnosis and epidemiological studies of the zoonotic disease toxoplasmosis. In the past 2 decades, molecular methods based on a variety of genetic markers have been developed, each with its advantages and limitations. The application of these methods has generated invaluable information to enhance our understanding of the epidemiology, population genetics and phylogeny ofT. gondii. However, since most studies focused solely on the detection but not genetic characterization ofT. gondii, the information obtained was limited. In this review, we discuss some widely used molecular methods and propose an integrated approach for the detection and identification ofT. gondii, in order to generate maximum information for epidemiological, population and phylogenetic studies of this key pathogen.

Molecular detection and identification ofDasheen mosaic virusinfectingAmorphophallus paeoniifolius

2011 ◽  
Vol 44 (13) ◽  
pp. 1248-1260 ◽  
Author(s):  
Binoy Babu ◽  
Vinayaka Hegde ◽  
T. Makeshkumar ◽  
M. L. Jeeva
Keyword(s):  
Molecular Detection ◽  
Detection And Identification

scholarly journals Molecular detection and identification of Rickettsiales pathogens in dog ticks from Costa Rica

2016 ◽  
Vol 7 (6) ◽  
pp. 1198-1202 ◽  
Author(s):  
Liliana Campos-Calderón ◽  
Leyda Ábrego-Sánchez ◽  
Antony Solórzano-Morales ◽  
Alberto Alberti ◽  
Gessica Tore ◽  
...  
Keyword(s):  
Costa Rica ◽  
Molecular Detection ◽  
Detection And Identification

scholarly journals DETECTION AND IDENTIFICATION OF YELLOW MOSAIC STUNT DISEASE ON Petunia sp. USING NESTED PCR METHOD

2021 ◽  
Vol 21 (1) ◽  
pp. 56-62
Author(s):  
Suryani Titi Astuti ◽  
Sri Sulandari ◽  
Sedyo Hartono ◽  
Susamto Somowiyarjo
Keyword(s):  
Electron Microscope ◽  
Nested Pcr ◽  
Molecular Detection ◽  
Ornamental Plant ◽  
Mechanical Transmission ◽  
Chenopodium Amaranticolor ◽  
Virus Particles ◽  
Central Java ◽  
Detection And Identification ◽  
Pcr Method

Detection and identification of yellow mosaic stunt disease on Petunia sp. using nested PCR method. Yellow mosaic stuntdisease was found at some nurseries of Petunia in Sleman, Yogyakarta, also in Muntilan and Magelang Central Java. Thedisease was very important due to its ability reducing the quality and quantity of Petunia seedlings. The causal agent of thedisease may be carried over to imported seeds and necessary to identify as a basic information for developing controlstrategies. This research was done by mechanical transmission on indicator plants. The observation of the causal agents wasconducted using electron microscope with quick dipping method and the molecular detection was done using nested PCRwith TobRT up1-TobRT do2 as the external primers and TobN up3-TobN do4 as the internal primers. Mechanical inoculationshowed chlorosis symptoms that developed into local spot on Chenopodium amaranticolor as well as mosaic and veinbanding on Nicotiana benthamiana. The observation using electron microscope showed rod-shaped virus particles sizedapproximately 300 nm and by PCR method produced around 568 bp and 400 bp DNA band. Based on the sequence analysis,the disease was caused by Rehmania mosaic virus. This type of Tobamovirus has 96% similarity with ReMV-Japan. ReMV, aplant pathogen which was a member of Tobamovirus that has never been reported in Indonesia. This research was the firstreport of ReMV in Indonesia infecting Petunia as ornamental plant.

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