First report on molecular detection and identification of Tomato aspermy virus naturally occurring on gladiolus in India

2011 ◽  
Vol 39 (3) ◽  
pp. 303-307 ◽  
Author(s):  
S. K. Raj ◽  
S. Kumar ◽  
D. K. Verma ◽  
S. K. Snehi
Keyword(s):  
Molecular Detection ◽  
First Report ◽  
Tomato Aspermy Virus ◽  
Naturally Occurring ◽  
Detection And Identification

scholarly journals First Report of Malva vein clearing virus Naturally Occurring in Hollyhock in Germany

Plant Disease ◽  
2010 ◽  
Vol 94 (2) ◽  
pp. 276-276 ◽  
Author(s):  
W. Menzel ◽  
S. Winter ◽  
K. R. Richert-Pöggeler
Keyword(s):  
Host Range ◽  
Direct Sequencing ◽  
Amino Acid Sequences ◽  
Rt Pcr ◽  
Electron Microscopic ◽  
First Report ◽  
Vein Clearing ◽  
Naturally Occurring ◽  
Potyvirus Species ◽  
Cloned Cdna

Hollyhocks are popular garden plants and selected cultivars of Alcea rosea (family Malvaceae) are widespread in Germany. In spring 2009, dozens of A. rosea plants displaying strong vein clearing and veinal yellowing symptoms were found in private gardens in Hannover, Lower Saxony. Electron microscopic examinations of negatively stained adsorption preparations of five randomly selected samples of symptomatic plants or their offshoots revealed flexuous filamentous particles resembling those of potyviruses. Sap extracts also reacted strongly positive in an antigen coated plate (ACP)-ELISA with the broad-spectrum potyvirus antiserum AS-0573/I (DSMZ, Braunschweig, Germany). RNA extracts (RNeasy Kit, Qiagen, Valencia, CA) of the above mentioned leaf samples were used as templates in reverse transcription (RT)-PCR assays with potyvirus specific primers (2) that have been shown to amplify the 3′ terminus of the genome of many potyvirus species. For extracts from symptomatic samples, this resulted in a consistent amplification of an ~1.6-kbp fragment, whereas no products were obtained from RNA extracts of asymptomatic plants. From one positive sample, the amplified fragment was cloned and one clone was partially sequenced. The nucleotide (nt) and amino acid sequences showed the highest identities (81 to 83% and 87 to 90%, respectively) to GenBank sequences FJ539084, FM212972, EU884405, and FJ561293 of the potyvirus Malva vein clearing virus (MVCM). On the basis of these identity values and according to the species demarcation criteria in the genus Potyvirus, the virus can be regarded as a German isolate of the recently sequenced MVCV (3,4). Direct sequencing of the 5′-end of the amplified RT-PCR fragment revealed sequences of only one potyvirus species. The virus isolate has been submitted to the DSMZ Plant Virus Collection (Braunschweig, Germany) under accession PV-0963 and the sequence obtained from the cloned cDNA is deposited in GenBank (GQ856544). In addition, sap from affected leaves was mechanically inoculated onto sets of herbaceous indicator plants (Chenopodium quinoa, C. foliosum, C. murale, C. amaranticolor, Datura stramonium, Nicotiana benthamiana, N. hesperis, Petunia hybrida, and Solanum lycopersicum) of which only C. quinoa plants became infected. Symptoms of weak chlorosis along and beside veins of inoculated leaves, but not systemic leaves, became visible 2 weeks postinoculation. Symptomatic leaves contained flexuous filamentous particles and ACP-ELISA and RT-PCR confirmed virus presence. The partially sequenced amplicon showed 99% nt identity to the sequence from the cloned cDNA. To our knowledge, this is the first report of a MVCV isolate naturally occurring in A. rosea and C. quinoa is the first host identified that does not belong to the plant family Malvaceae. In contrast, the MVCV isolate used in the host range study of Lunello et al. (4) did not infect A. rosea and C. quinoa, confirming previous host range descriptions by Brunt et al. (1). Since MVCV infections of hollyhocks seem to cause only leaf symptoms and do not noticeably affect growth or flowering of the plants, this will hopefully not impair the usability of this popular garden plant. References: (1) A. A. Brunt et al. Descriptions and Lists from the VIDE Database. Online publication. Version: 16th January, 1997. (2) J. Chen et al. Arch. Virol. 146:757, 2001. (3) A. Hein Phytopathol. Z. 28:205, 1957. (4) P. Lunello et al. Virus Res. 140:91, 2009.

scholarly journals First Report of Fragaria chiloensis cryptic virus, Fragaria chiloensis latent virus, Strawberry mild yellow edge virus, Strawberry necrotic shock virus, and Strawberry pallidosis associated virus in Single and Mixed Infections in Strawberry in Central Mexico

Plant Disease ◽  
2013 ◽  
Vol 97 (7) ◽  
pp. 1002-1002 ◽  
Author(s):  
L. Silva-Rosales ◽  
M. N. Vázquez-Sánchez ◽  
V. Gallegos ◽  
M. L. Ortiz-Castellanos ◽  
R. Rivera-Bustamante ◽  
...  
Keyword(s):  
Viral Infections ◽  
The United States ◽  
Latent Virus ◽  
Nucleotide Sequencing ◽  
Mixed Infections ◽  
Cryptic Virus ◽  
First Report ◽  
Detection And Identification ◽  
Two Samples ◽  
Fragaria Chiloensis

For phytosanitary purposes, the prevalence and incidence of viruses found in strawberry production within a centralized breeding program was investigated in Abasolo and Irapuato Counties, Guanajuato State, Mexico. Single and mixed infections of Strawberry mottle virus (SMoV) and Strawberry crinkle virus (SCV) were originally reported in the area (3), and subsequently, Strawberry latent ringspot virus (SLRSV) was also found (4). Samples of strawberry plants showing viral symptoms: stunting, mild chlorosis and reddening, occasional wrinkled, curled, and deformed leaves that may exhibit mottling, and chlorotic spots, forming a putative virus complex were collected in April and December 2007 and July and December 2008. The detection and identification of viruses reported in the United States, the country of origin of most of the imported plantlets, was carried out with sets of primers for 11 viruses, through reverse transcription (RT)-PCR (developed by Robert Martin and Ioannis Tzanetakis in Corvallis, OR). The endogenous NADH 2 subunit was employed to test the quality of the RNA extracted. Amplification conditions were: 40 cycles of 1 min at each temperature, denaturation at 95°C, annealing at 50°C for Strawberry necrotic shock virus (SNSV); 52°C for Strawberry mild yellow edge virus (SMYEV); 55°C for Fragaria chiloensis latent virus (FClLV), Strawberry pallidosis associated virus (SPaV), Fragaria chiloensis cryptic virus (FClCV), and SMoV; and 58°C for SCV and NADH dehydrogenase, followed by a final extension at 72°C of 5 min after completion of the 40 cycles. The cloning and nucleotide sequencing of amplified fragments revealed the presence of seven viral species in 40 samples collected. These were FClLV, SCV, SMoV, SNSV, SPaV, and SMYEV, which were allocated GenBank accession numbers of JQ629412, JQ629413, JQ629414, JQ629415, JQ629416, and JQ629417, respectively. Strawberry UC-4 and UC-10 (1,2) were planted as indicators of viral infections on an experimental plot. All seven viruses were detected in single or mixed infections. SMoV was the most commonly found in combination with other viruses. Out of 40 samples, 35 were positive for the presence of viruses and six had single infections, of which five had SMoV and one had SPaV. The remaining 29 samples had mixed infections with two or more viruses in a total of 22 combinations. The combination of FCICV + SMoV was present in five samples, whereas the combination of SMoV + SMYEV was in two samples. All other samples had two and up to six different viruses per plant. SMoV was detected in 26 out of the 40 samples tested. SNSV and FClCV were detected in 14 samples. SMYEV was present in 13 samples. SCV was present in nine samples, whereas SPaV and FClLV were found in eight samples each. To the best of our knowledge, this is the first report of FClLV, FClCV, SNSV, SMYEV, and SPaV in Mexico. References: (1) N. W. Frazier. Plant Dis. Rep. 58:28, 1974. (2) N. W. Frazier. Plant Dis. Rep. 58:203, 1974. (3) D. Teliz-Ortiz and A. Trejo-Reyes. Rev. Mex. Fitopatol. 7:38, 1989. (4) L. Pérez-Moreno et al. Rev. Mex. Fitopatol. 22:187, 2004.

First molecular detection and identification of Trypanosoma evansi in goats from Cebu, Philippines using a PCR-based assay

2020 ◽  
Vol 21 ◽  
pp. 100414
Author(s):  
Afraa Elata ◽  
Eloiza May Galon ◽  
Paul Franck Adjou Moumouni ◽  
Rochelle Haidee D. Ybanez ◽  
Ehab Mossaad ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Trypanosoma Evansi ◽  
Detection And Identification

scholarly journals Molecular detection and identification of Rickettsia endosymbiont in different biotypes of Bemisia tabaci

2009 ◽  
Vol 15 ◽  
pp. 271-272 ◽  
Author(s):  
G. Gueguen ◽  
J.M. Rolain ◽  
E. Zchori-Fein ◽  
F. Vavre ◽  
F. Fleury ◽  
...  
Keyword(s):  
Bemisia Tabaci ◽  
Molecular Detection ◽  
Detection And Identification

Molecular detection and identification of hemoparasites in pampas deer (Ozotoceros bezoarticus Linnaeus, 1758) from the Pantanal Brazil

2013 ◽  
Vol 4 (4) ◽  
pp. 341-345 ◽  
Author(s):  
Júlia A.G. Silveira ◽  
Élida M.L. Rabelo ◽  
Ana C.R. Lacerda ◽  
Paulo A.L. Borges ◽  
Walfrido M. Tomás ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Ozotoceros Bezoarticus ◽  
Detection And Identification

scholarly journals Molecular Detection and Identification of Rickettsia Species in Ixodes pacificus in California

2011 ◽  
Vol 11 (7) ◽  
pp. 957-961 ◽  
Author(s):  
Jimmy Ninh Phan ◽  
Casey Roy Lu ◽  
William Garrett Bender ◽  
Robert Marion Smoak ◽  
Jianmin Zhong
Keyword(s):  
Molecular Detection ◽  
Ixodes Pacificus ◽  
Rickettsia Species ◽  
Detection And Identification

Moving towards an integrated approach to molecular detection and identification ofToxoplasma gondii

Parasitology ◽  
2009 ◽  
Vol 137 (1) ◽  
pp. 1-11 ◽  
Author(s):  
C. SU ◽  
E. K. SHWAB ◽  
P. ZHOU ◽  
X. Q. ZHU ◽  
J. P. DUBEY
Keyword(s):  
Molecular Detection ◽  
Genetic Characterization ◽  
Integrated Approach ◽  
Epidemiological Studies ◽  
Molecular Methods ◽  
Maximum Information ◽  
The Past ◽  
Phylogenetic Studies ◽  
Detection And Identification

SUMMARYThe development of simple, sensitive and rapid methods for the detection and identification ofToxoplasma gondiiis important for the diagnosis and epidemiological studies of the zoonotic disease toxoplasmosis. In the past 2 decades, molecular methods based on a variety of genetic markers have been developed, each with its advantages and limitations. The application of these methods has generated invaluable information to enhance our understanding of the epidemiology, population genetics and phylogeny ofT. gondii. However, since most studies focused solely on the detection but not genetic characterization ofT. gondii, the information obtained was limited. In this review, we discuss some widely used molecular methods and propose an integrated approach for the detection and identification ofT. gondii, in order to generate maximum information for epidemiological, population and phylogenetic studies of this key pathogen.

First Report of Tomato Aspermy Virus in Arkansas

Plant Disease ◽  
1986 ◽  
Vol 70 (11) ◽  
pp. 1074c ◽  
Author(s):  
I. B. Ahmad
Keyword(s):  
First Report ◽  
Tomato Aspermy Virus

Molecular detection and identification ofDasheen mosaic virusinfectingAmorphophallus paeoniifolius

2011 ◽  
Vol 44 (13) ◽  
pp. 1248-1260 ◽  
Author(s):  
Binoy Babu ◽  
Vinayaka Hegde ◽  
T. Makeshkumar ◽  
M. L. Jeeva
Keyword(s):  
Molecular Detection ◽  
Detection And Identification
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