Moving towards an integrated approach to molecular detection and identification ofToxoplasma gondii

Parasitology ◽  
2009 ◽  
Vol 137 (1) ◽  
pp. 1-11 ◽  
Author(s):  
C. SU ◽  
E. K. SHWAB ◽  
P. ZHOU ◽  
X. Q. ZHU ◽  
J. P. DUBEY
Keyword(s):  
Molecular Detection ◽  
Genetic Characterization ◽  
Integrated Approach ◽  
Epidemiological Studies ◽  
Molecular Methods ◽  
Maximum Information ◽  
The Past ◽  
Phylogenetic Studies ◽  
Detection And Identification

SUMMARYThe development of simple, sensitive and rapid methods for the detection and identification ofToxoplasma gondiiis important for the diagnosis and epidemiological studies of the zoonotic disease toxoplasmosis. In the past 2 decades, molecular methods based on a variety of genetic markers have been developed, each with its advantages and limitations. The application of these methods has generated invaluable information to enhance our understanding of the epidemiology, population genetics and phylogeny ofT. gondii. However, since most studies focused solely on the detection but not genetic characterization ofT. gondii, the information obtained was limited. In this review, we discuss some widely used molecular methods and propose an integrated approach for the detection and identification ofT. gondii, in order to generate maximum information for epidemiological, population and phylogenetic studies of this key pathogen.

Molecular detection and genetic characterization of ovine gammaherpesvirus-2 (OvHV-2) in sheep in the Philippines

2021 ◽  
Vol 199 ◽  
pp. 106383
Author(s):  
Innina R. Mananguit ◽  
Nest Dale F. Bartolome ◽  
Gabriel Alexis S.P. Tubalinal ◽  
Claro N. Mingala
Keyword(s):  
Molecular Detection ◽  
Genetic Characterization ◽  
The Philippines

scholarly journals First molecular detection and genetic characterization of Coxiella burnetii in Zambian dogs and rodents

2018 ◽  
Vol 11 (1) ◽  
Author(s):  
Simbarashe Chitanga ◽  
Edgar Simulundu ◽  
Martin C. Simuunza ◽  
Katendi Changula ◽  
Yongjin Qiu ◽  
...  
Keyword(s):  
Coxiella Burnetii ◽  
Molecular Detection ◽  
Genetic Characterization

scholarly journals Genetic characterization of a Neisseria meningitidis cluster in Queensland, Australia

2017 ◽  
Vol 63 (7) ◽  
pp. 644-647
Author(s):  
N.R. Abady ◽  
C.J.D. Guglielmino ◽  
R.M. Graham ◽  
J. Adelskov ◽  
H.V. Smith ◽  
...  
Keyword(s):  
Neisseria Meningitidis ◽  
Meningococcal Disease ◽  
Core Genome ◽  
Molecular Detection ◽  
Genetic Characterization ◽  
Genomic Analysis ◽  
Rural Region ◽  
Disease Cluster ◽  
Sporadic Disease

Neisseria meningitidis serogroups B and C have been responsible for the majority of invasive meningococcal disease in Australia, with serogroup B strains causing an increasing proportion of cases in recent years. Serogroup Y has typically caused sporadic disease in Australia. In 2002, a cluster of 4 cases was reported from a rural region in Queensland. Three of these cases were serogroup C, with 1 case diagnosed by molecular detection only, and the fourth case was identified as a serogroup Y infection. Genomic analysis, including antigen finetyping, multilocus sequence typing (MLST), and core genome MLST, demonstrated that the serogroup Y case, though spatially and temporally linked to a serogroup C disease cluster, was not the product of a capsule switch and that one of the serogroup C isolates had a deletion of the entire porA sequence.

Oropouche orthobunyavirus: Genetic characterization of full-length genomes and development of molecular methods to discriminate natural reassortments

2019 ◽  
Vol 68 ◽  
pp. 16-22 ◽  
Author(s):  
Márcio Roberto Teixeira Nunes ◽  
William Marciel de Souza ◽  
Nazir Savji ◽  
Mário Luís Figueiredo ◽  
Jedson Ferreira Cardoso ◽  
...  
Keyword(s):  
Genetic Characterization ◽  
Full Length ◽  
Molecular Methods

Molecular detection and genetic characterization of Toxoplasma gondii in pork in Shanghai, China

Food Control ◽  
2019 ◽  
Vol 99 ◽  
pp. 57-63 ◽  
Author(s):  
Yehua Zhang ◽  
Rongsheng Mi ◽  
Yan Huang ◽  
Luming Xia ◽  
Yushu Cai ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Molecular Detection ◽  
Genetic Characterization

scholarly journals Antigenic and genetic characterization of rabies viruses isolated from domestic and wild animals of Brazil identifies the hoary fox as a rabies reservoir

2005 ◽  
Vol 86 (11) ◽  
pp. 3153-3162 ◽  
Author(s):  
F. Bernardi ◽  
S. A. Nadin-Davis ◽  
A. I. Wandeler ◽  
J. Armstrong ◽  
A. A. B. Gomes ◽  
...  
Keyword(s):  
Animal Species ◽  
Genetic Characterization ◽  
Variable Region ◽  
Epidemiological Studies ◽  
Genetic Classification ◽  
Livestock Species ◽  
Central Variable Region ◽  
Vampire Bats

Fifty Brazilian rabies viruses, collected from many different animal species and several regions of the country, were characterized by partial sequencing of the central, variable region of the P gene, a locus useful for sensitive molecular epidemiological studies. Phylogenetic analysis of the sequences, which included comparison with other rabies strains recovered from throughout the Americas, identified three main groups of Brazilian viruses, arbitrarily designated BRL-1 to BRL-3. BRL-1 was found in terrestrial carnivores and clusters with other American strains of the cosmopolitan lineage. BRL-2 comprised two distinct isolates, recovered from two species of non-haematophagous bats, that had evolutionary links to insectivorous-bat-derived strains of North America. BRL-3 consisted of isolates from vampire bats and from livestock species probably infected via contact with vampire bats. The terrestrial group was further subdivided into three subtypes: BRL-1a was associated exclusively with dogs and cats, while BRL-1b and BRL-1c were found exclusively in hoary foxes. These observations strongly support the role of the Brazilian hoary fox as a rabies reservoir. Screening of representative Brazilian rabies viruses against a collection of anti-rabies monoclonal antibodies (mAbs) identified a small panel of mAbs that could be used to discriminate between all Brazilian subgroups as defined by genetic classification in this study.

scholarly journals Molecular Detection and Genetic Characterization of Toxoplasma gondii in Farmed Minks (Neovison vison) in Northern China by PCR-RFLP

PLoS ONE ◽  
2016 ◽  
Vol 11 (11) ◽  
pp. e0165308 ◽  
Author(s):  
Wen-Bin Zheng ◽  
Xiao-Xuan Zhang ◽  
Jian-Gang Ma ◽  
Fa-Cai Li ◽  
Quan Zhao ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Molecular Detection ◽  
Genetic Characterization ◽  
Northern China ◽  
Neovison Vison ◽  
Pcr Rflp
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