scholarly journals Lumpy skin disease: Attempted propagation in tick cell lines and presence of viral DNA in field ticks collected from naturally-infected cattle

2015 ◽  
Vol 6 (2) ◽  
pp. 134-140 ◽  
Author(s):  
E.S.M. Tuppurainen ◽  
E.H. Venter ◽  
J.A.W. Coetzer ◽  
L. Bell-Sakyi
Keyword(s):  
Cell Lines ◽  
Skin Disease ◽  
Infected Cattle ◽  
Tick Cell ◽  
Viral Dna ◽  
Lumpy Skin Disease

scholarly journals Serum Biochemistry of Lumpy Skin Disease Virus-Infected Cattle

2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Murat Şevik ◽  
Oğuzhan Avci ◽  
Müge Doğan ◽  
Ömer Barış İnce
Keyword(s):  
Real Time ◽  
Disease Virus ◽  
Real Time Pcr ◽  
Skin Disease ◽  
Infected Cattle ◽  
Serum Samples ◽  
Lumpy Skin Disease ◽  
Blood Samples ◽  
Lumpy Skin Disease Virus ◽  
Serum Biochemical

Lumpy skin disease is an economically important poxvirus disease of cattle. Vaccination is the main method of control but sporadic outbreaks have been reported in Turkey. This study was carried out to determine the changes in serum biochemical values of cattle naturally infected with lumpy skin disease virus (LSDV). For this study, blood samples in EDTA, serum samples, and nodular skin lesions were obtained from clinically infected animals (n=15) whereas blood samples in EDTA and serum samples were collected from healthy animals (n=15). A quantitative real-time PCR method was used to detectCapripoxvirus(CaPV) DNA in clinical samples. A real-time PCR high-resolution melt assay was performed to genotype CaPVs. Serum cardiac, hepatic, and renal damage markers and lipid metabolism products were measured by autoanalyzer. LSDV nucleic acid was detected in all samples which were obtained from clinically infected cattle. The results of serum biochemical analysis showed that aspartate aminotransferase, alkaline phosphatase, total protein, and creatinine concentrations were markedly increased in serum from infected animals. However, there were no significant differences in the other biochemical parameters evaluated. The results of the current study suggest that liver and kidney failures occur during LSDV infection. These findings may help in developing effective treatment strategies in LSDV infection.

scholarly journals Mechanical transmission of lumpy skin disease virus by Aedes aegypti (Diptera: Culicidae)

2001 ◽  
Vol 126 (2) ◽  
pp. 317-321 ◽  
Author(s):  
C. M. CHIHOTA ◽  
L. F. RENNIE ◽  
R. P. KITCHING ◽  
P. S. MELLOR
Keyword(s):  
Aedes Aegypti ◽  
Disease Virus ◽  
Skin Disease ◽  
Mosquito Species ◽  
Clinical Disease ◽  
Mechanical Transmission ◽  
Infected Cattle ◽  
Lumpy Skin Disease ◽  
Lumpy Skin Disease Virus

Aedes aegypti female mosquitoes are capable of the mechanical transmission of lumpy skin disease virus (LSDV) from infected to susceptible cattle. Mosquitoes that had fed upon lesions of LSDV-infected cattle were able to transmit virus to susceptible cattle over a period of 2–6 days post-infective feeding. Virus was isolated from the recipient animals in 5 out of 7 cases. The clinical disease recorded in the animals exposed to infected mosquitoes was generally of a mild nature, with only one case being moderate. LSDV has long been suspected to be insect transmitted, but these findings are the first to demonstrate this unequivocally, and they suggest that mosquito species are competent vectors.

Detection of lumpy skin disease virus in saliva of ticks fed on lumpy skin disease virus-infected cattle

2013 ◽  
Vol 61 (1) ◽  
pp. 129-138 ◽  
Author(s):  
J. C. Lubinga ◽  
E. S. M. Tuppurainen ◽  
W. H. Stoltsz ◽  
K. Ebersohn ◽  
J. A. W. Coetzer ◽  
...  
Keyword(s):  
Disease Virus ◽  
Skin Disease ◽  
Infected Cattle ◽  
Lumpy Skin Disease ◽  
Lumpy Skin Disease Virus

scholarly journals Virological, Molecular And Immuno-Biochemical Studies Of Lumpy Skin Disease In Naturally Infected Cattle

2021 ◽  
Vol 6 (1) ◽  
pp. 28-37
Author(s):  
morcos yanni ◽  
Ibtisam Gamal Elden ◽  
Nahed Kamoura ◽  
Mohamed Ibrahim
Keyword(s):  
Skin Disease ◽  
Infected Cattle ◽  
Lumpy Skin Disease ◽  
Biochemical Studies

scholarly journals Diagnosis of naturally occurring lumpy skin disease virus infection in cattle using virological, molecular, and immunohistopathological assays

2021 ◽  
pp. 2230-2237
Author(s):  
Dawlat M. Amin ◽  
Gehan Shehab ◽  
Rawhya Emran ◽  
Rabab T. Hassanien ◽  
Gehan N. Alagmy ◽  
...  
Keyword(s):  
Skin Disease ◽  
Molecular Detection ◽  
Chorioallantoic Membrane ◽  
Hair Follicles ◽  
Confirmatory Test ◽  
Economic Losses ◽  
Infected Cattle ◽  
Basal Cells ◽  
Lumpy Skin Disease ◽  
Skin Nodule

Background and Aim: Lumpy skin disease (LSD) is a contagious viral disease that has great economic losses among Egyptian breeding flocks. The present study was designed to compare the results of different diagnostic approaches used for the diagnosis of LSD virus (LSDV). Materials and Methods: A total of 73 skin nodule samples were collected from suspected infected cattle with LSDV from some Egyptian governorates during 2019 and 2020. Trials for virus isolation (VI) and identification on embryonated chicken eggs (ECEs) were conducted. Molecular detection, histopathological, and immunohistochemical examination were also conducted. Results: The virus was isolated into ECEs, and 58 samples of 73 were positive and gave a characteristic pock lesion on the chorioallantoic membrane. Twenty-two representative nodular skin specimens of the 58 positive samples were selected to be used for molecular, histopathological, and immunohistochemistry (IHC) diagnosis. Conventional polymerase chain reaction succeeded in detecting LSDV DNA in all tested 22 skin nodule samples. Histological examination of skins of different cases revealed various alterations depending on the stage of infection. IHC was used as a confirmatory test for detecting LSDV antigen in the tissues of the skin nodules of infected cattle using specific anti-LSDV antibodies. Lumpy skin viral antigen was detected within the cytoplasm of the epidermal basal cells layer and prickle cell and within the cytoplasm of the hair follicles' epithelial outer and inner roots. Conclusion: This study confirmed the prevalence of LSDV infection in different Egyptian governorates during 2019 and 2020. In addition, histopathology and IHC could be potential methods to confirm Lumpy skin disease infection besidesVI and molecular detection.

scholarly journals Lumpy skin disease virus does not replicate productively in insect cell lines

2019 ◽  
Vol 1 (10) ◽  
Author(s):  
Charlotte Cook ◽  
Isabelle Dietrich ◽  
David Greaves ◽  
Karin Darpel ◽  
Pip Beard
Keyword(s):  
Disease Virus ◽  
Cell Lines ◽  
Skin Disease ◽  
Insect Cell ◽  
Lumpy Skin Disease ◽  
Lumpy Skin Disease Virus ◽  
Insect Cell Lines
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