Real-time PCR assay with an endogenous internal amplification control for detection and quantification of Anaplasma marginale in bovine blood

2020 ◽  
Vol 11 (2) ◽  
pp. 101334
Author(s):  
Svetlana N. Kovalchuk ◽  
Anna V. Babii ◽  
Anna L. Arkhipova
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Anaplasma Marginale ◽  
Internal Amplification Control ◽  
Bovine Blood ◽  
Pcr Assay ◽  
Detection And Quantification

scholarly journals Detection of Peanut Allergen Traces with a Real Time PCR Assay - The Challenge to Protect Food-Allergic Consumers

2017 ◽  
Vol 7 (1) ◽  
pp. 32 ◽  
Author(s):  
Dimitra Houhoula ◽  
Stamatios Koussissis ◽  
Vladimiros Lougovois ◽  
John Tsaknis ◽  
Dimitra Kassavita ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Food Products ◽  
Molecular Techniques ◽  
Food Allergens ◽  
Pcr Assay ◽  
Peanut Allergen ◽  
Pcr Method ◽  
Detection And Quantification

The aim of the present study was the implementation of molecular techniques in the detection and quantification of allergic substances of peanut in various kinds of food products, e.g., breakfast cereals, chocolates and biscuits that are frequently related to allergies. In some cases, the presence of peanuts can be due to contamination during production and are not declared on the label. A total of 152 samples were collected from supermarkets and were analysed by a Real Time PCR method. The results indicated that 125 samples (83,3%) were found positive in peanut traces but the most important finding is that from the 84 samples that had no allergen declaration for peanuts, 48 (57,1%) of them were found positive. In conclusion, Real Time PCR can be a very important tool for the rapid detection and quantification of food allergens.

scholarly journals One-step real-time PCR assay for detection and quantification of RNA HCV to monitor patients under treatment in Brazil

2018 ◽  
Vol 22 (5) ◽  
pp. 418-423
Author(s):  
Elisabete Andrade ◽  
Daniele Rocha ◽  
Marcela Fontana-Maurell ◽  
Elaine Costa ◽  
Marisa Ribeiro ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Pcr Assay ◽  
One Step ◽  
Detection And Quantification

A real time PCR assay for the detection and quantification of orf virus

2006 ◽  
Vol 134 (1-2) ◽  
pp. 140-145 ◽  
Author(s):  
L. Gallina ◽  
F. Dal Pozzo ◽  
C.J. Mc Innes ◽  
G. Cardeti ◽  
A. Guercio ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Orf Virus ◽  
Pcr Assay ◽  
Detection And Quantification

scholarly journals Real-Time PCR Assay for Detection and Quantification of Hepatitis B Virus Genotypes A to G

2006 ◽  
Vol 44 (9) ◽  
pp. 3325-3333 ◽  
Author(s):  
T. M. Welzel ◽  
W. J. Miley ◽  
T. L. Parks ◽  
J. J. Goedert ◽  
D. Whitby ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Real Time ◽  
Real Time Pcr ◽  
Pcr Assay ◽  
B Virus ◽  
Virus Genotypes ◽  
Detection And Quantification

Development of a real-time PCR assay with an internal amplification control for detection of Gram-negative histamine-producing bacteria in fish

2011 ◽  
Vol 28 (3) ◽  
pp. 356-363 ◽  
Author(s):  
Kristin Bjornsdottir-Butler ◽  
Jessica L. Jones ◽  
Ronald Benner ◽  
William Burkhardt
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Internal Amplification Control ◽  
Pcr Assay ◽  
Gram Negative

Rapid detection of Cronobacter sakazakii by real-time PCR based on the cgcA gene and TaqMan probe with internal amplification control

2016 ◽  
Vol 62 (3) ◽  
pp. 191-200 ◽  
Author(s):  
Shuangfang Hu ◽  
Yigang Yu ◽  
Rong Li ◽  
Xinwei Wu ◽  
Xinglong Xiao ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Virulent Strain ◽  
Internal Amplification Control ◽  
Taqman Probe ◽  
Powdered Infant Formula ◽  
Cronobacter Sakazakii ◽  
Pcr Assay ◽  
Lactobacillus Pentosus ◽  
Qrt Pcr

Cronobacter sakazakii is a severe virulent strain that is frequently detected in powdered infant formula (PIF). Therefore, it is necessary to develop a fast and specific detection method. The specificity of our newly developed quantitative real-time PCR (qRT–PCR) was validated with DNA from 46 strains. Among them, 12 C. sakazakii strains were correctly amplified, whereas no positive florescent signal was observed from 34 nontarget controls. The detection limit of C. sakazakii was about 110 CFU/mL in broth and 1100 CFU/g in PIF. After enrichment in buffered peptone water for 6 h, our developed qRT–PCR assay could reliably detect C. sakazakii when the inoculation level was as low as 2 CFU/25 g (0.08 CFU/g) in PIF. The growth of C. sakazakii could be inhibited by the presence of Lactobacillus pentosus and Bacillus cereus, which used a longer enrichment period before the isolation was accomplished. However, at 5 and 50 CFU/25 g inoculation levels of C. sakazakii in the presence of 4 × 106 CFU L. pentosus/25 g or of 2 × 104 CFU B. cereus/25 g, the qRT–PCR assay could detect the presence of Cronobacter even though these artificially spiked samples were negative in culture. Therefore, our results indicated that the qRT–PCR assay could detect samples containing inhibitors and could avoid false negatives by using an internal amplification control.

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