Improved detection of Babesia bigemina from various geographical areas in Africa using quantitative PCR and reverse line blot hybridisation

2020 ◽  
Vol 11 (4) ◽  
pp. 101415
Author(s):  
Hein Stoltsz ◽  
Charles Byaruhanga ◽  
Milana Troskie ◽  
Marcus Makgabo ◽  
Marinda C. Oosthuizen ◽  
...  
Keyword(s):  
Quantitative Pcr ◽  
Reverse Line Blot ◽  
Babesia Bigemina ◽  
Blot Hybridisation

scholarly journals Reverse line blot hybridisation screening of Pseudallescheria/Scedosporium species in patients with cystic fibrosis

Mycoses ◽  
2011 ◽  
Vol 54 ◽  
pp. 5-11 ◽  
Author(s):  
Q. Lu ◽  
A. H. G. Gerrits van den Ende ◽  
G. S. de Hoog ◽  
R. Li ◽  
I. Accoceberry ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Reverse Line Blot ◽  
Blot Hybridisation ◽  
Scedosporium Species

Genotyping of human adenoviruses using a PCR-based reverse line blot hybridisation assay

Pathology ◽  
2011 ◽  
Vol 43 (5) ◽  
pp. 488-494 ◽  
Author(s):  
Yongyan Cao ◽  
Fanrong Kong ◽  
Fei Zhou ◽  
Meng Xiao ◽  
Qinning Wang ◽  
...  
Keyword(s):  
Reverse Line Blot ◽  
Human Adenoviruses ◽  
Blot Hybridisation

scholarly journals A Comparison of Peripheral Blood Smears, Autologous Cell Cultures, and Reverse Line Blot Hybridisation in Screening for Anaplasma/Ehrlichia in Roaming Dogs and Symptomatic Dogs in Trinidad

Pathogens ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 1431
Author(s):  
Karla Georges ◽  
Chuckwudozi Ezeokoli ◽  
Godwin Isitor ◽  
Alex Mutani ◽  
Olivier Sparagano ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Cultures ◽  
Inclusion Bodies ◽  
Clinical Signs ◽  
Reverse Line Blot ◽  
Rrna Gene ◽  
Canine Ehrlichiosis ◽  
Autologous Cell ◽  
Blot Hybridisation ◽  
Species Specific

This study compared two methods to detect cases of canine ehrlichiosis in a field setting. One method was a polymerase chain reaction for the 16S rRNA gene followed by reverse line blot hybridisation with genera and species-specific probes for Anaplasma/Ehrlichia. The second method was an autologous cell culture of peripheral leucocytes isolated from heparinised blood and maintained in a homologous canine serum in Dulbecco’s Modified Eagle medium without antibiotics. The cultures were examined under light microscopy for inclusion bodies after 48 h. Leucocytes were successfully propagated for 20 of the 34 samples submitted for autologous cell culture. Inclusion bodies were observed after cell culture in leucocytes of eight dogs. Two dogs were positive to the Anaplasma/Ehrlichia genera probe and six dogs were positive to the E. canis probe after reverse line blot hybridisation. There was acceptable agreement between reverse line blot hybridisation and cell culture results. Both reverse line blot hybridisation and autologous cell cultures can be used to detect E. canis in subclinical and clinical cases of disease. A definitive diagnosis of E. canis is best achieved by a combination of clinical signs, positive autologous cell culture, and reverse line blot hybridisation results.

scholarly journals Identification of Trypanosoma evansi by DNA hybridisation using a non-radioactive probe generated by arbitrary primer PCR: Short communication

2001 ◽  
Vol 49 (2) ◽  
pp. 191-195
Author(s):  
S. H. Basagoudanavar ◽  
J. R. Rao ◽  
Swati Omanwar ◽  
A. K. Tiwari ◽  
R. K. Singh ◽  
...  
Keyword(s):  
Trypanosoma Evansi ◽  
Babesia Bigemina ◽  
Dot Blot ◽  
Arbitrary Primer ◽  
Inherent Risk ◽  
Dna Hybridisation ◽  
Blot Hybridisation ◽  
Polymerase Chain ◽  
Primer Polymerase Chain Reaction ◽  
Template Dna

A highly reproducible, dominant, monomorphic fragment of 473 base pair (bp) amplified from the genome of Trypanosoma evansi by arbitrary primer — polymerase chain reaction (AP-PCR) was labelled with digoxigenin and investigated for its potential as DNA probe. Dot-blot hybridisation of total genomic DNA with the probe proved useful in detecting bubaline, cameline and equine strains of T. evansi down to 10 pg of parasite template DNA. No cross-hybridisation was seen with Babesia bigemina, Theileria annulata and the bubaline host DNA. This probe may facilitate laboratory identification of T. evansi in developing countries, without the inherent risk associated with radioisotopes.

Application of a Reverse Line Blot hybridisation assay for the species-specific identification of cyathostomins (Nematoda, Strongylida) from benzimidazole-treated horses in the Slovak Republic

2009 ◽  
Vol 160 (1-2) ◽  
pp. 171-174 ◽  
Author(s):  
Dana Čerňanská ◽  
Barbara Paoletti ◽  
Ivica Kráľová-Hromadová ◽  
Raffaella Iorio ◽  
Patrícia Čudeková ◽  
...  
Keyword(s):  
Slovak Republic ◽  
Reverse Line Blot ◽  
Specific Identification ◽  
Blot Hybridisation ◽  
Species Specific

Detection of haemoparasites in cattle by reverse line blot hybridisation with a note on the distribution of ticks in Sicily

2001 ◽  
Vol 99 (4) ◽  
pp. 273-286 ◽  
Author(s):  
K Georges ◽  
G.R Loria ◽  
S Riili ◽  
A Greco ◽  
S Caracappa ◽  
...  
Keyword(s):  
Reverse Line Blot ◽  
Blot Hybridisation

Konsolidierungstherapie beim follikulären Lymphom: Längeres Überleben ohne Progress

2010 ◽  
Vol 01 (03) ◽  
pp. 144-144
Author(s):  
Alexander Kretzschmar
Keyword(s):  
Quantitative Pcr ◽  
Ibritumomab Tiuxetan

Der Vorteil einer Konsolidierungstherapie mit 90Yttrium-Ibritumomab-Tiuxetan (Zevalin®) beim follikulären Lymphom (FL) wurde in der FIT-Studie anhand der Verbesserung des progressionsfreien Überlebens belegt. Eine quantitative PCR-Analyse (qPCR) der Studie zeigt nun einen weiteren Vorteil der Konsolidierung mit 90Yttrium- Ibritumomab-Tiuxetan: Der Antikörper führt bei 90% der initial bcl-2- positiven Patienten zur bcl-2- Negativität und parallel zu einer Verlängerung des progressionsfreien Überlebens auf über 40 Monate.

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