scholarly journals Identification of Trypanosoma evansi by DNA hybridisation using a non-radioactive probe generated by arbitrary primer PCR: Short communication

2001 ◽  
Vol 49 (2) ◽  
pp. 191-195
Author(s):  
S. H. Basagoudanavar ◽  
J. R. Rao ◽  
Swati Omanwar ◽  
A. K. Tiwari ◽  
R. K. Singh ◽  
...  
Keyword(s):  
Trypanosoma Evansi ◽  
Babesia Bigemina ◽  
Dot Blot ◽  
Arbitrary Primer ◽  
Inherent Risk ◽  
Dna Hybridisation ◽  
Blot Hybridisation ◽  
Polymerase Chain ◽  
Primer Polymerase Chain Reaction ◽  
Template Dna

A highly reproducible, dominant, monomorphic fragment of 473 base pair (bp) amplified from the genome of Trypanosoma evansi by arbitrary primer — polymerase chain reaction (AP-PCR) was labelled with digoxigenin and investigated for its potential as DNA probe. Dot-blot hybridisation of total genomic DNA with the probe proved useful in detecting bubaline, cameline and equine strains of T. evansi down to 10 pg of parasite template DNA. No cross-hybridisation was seen with Babesia bigemina, Theileria annulata and the bubaline host DNA. This probe may facilitate laboratory identification of T. evansi in developing countries, without the inherent risk associated with radioisotopes.

scholarly journals DIRECT AND SENSITIVE DETECTION OF TRYPANOSOMA EVANSI BY POLYMERASE CHAIN REACTION

1999 ◽  
Vol 47 (3) ◽  
pp. 351-359 ◽  
Author(s):  
S. Omanwar ◽  
J. R. Rao ◽  
G. Butchaiah ◽  
S. H. Basagoudanavar ◽  
R. K. Singh
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Sequences ◽  
Animal Health ◽  
Trypanosoma Evansi ◽  
Chain Reaction ◽  
Wasting Disease ◽  
Specificity And Sensitivity ◽  
Sensitivity Level ◽  
Polymerase Chain ◽  
Template Dna

The mechanically transmitted haemoflagellate,Trypanosoma evansicauses 'surra', a wasting disease of domestic animals and is highly endemic in distribution in Southeast Asia. The detection ofT. evansiis important for improving the epizootiological and animal health status of the region. The specificity and sensitivity of polymerase chain reaction (PCR) using oligonucleotide primers constructed fromT. evansirepetitive DNA sequences were studied in the present investigation. Using the assay, it was possible to amplify template DNA ofT. evansiderived from buffaloes, camels and horses to a threshold sensitivity level of 0.5 pg and to detect DNA from as few as five organisms in 10 (l crude blood samples. Following experimental infection of calves with 5 × 105T. evansi, positive signals could be observed as early as 12 h post-infection. DNAs from two common haemoflagellates of cattle,Babesia bigeminaandTheileria annulatawere not amplified with the primers.

scholarly journals Detection of viruses in the exotic shrimp Penaeus vannamei Boone, 1931 cultured in India

2017 ◽  
Vol 64 (2) ◽  
Author(s):  
Y. Moger Rajeish ◽  
G. NarasimhaMurthy ◽  
Hoovinahalli Nataraju Madhushree ◽  
Basavareddy R. Pujar ◽  
Shivani Kallappa Girisha ◽  
...  
Keyword(s):  
Clinical Signs ◽  
Pacific White Shrimp ◽  
White Shrimp ◽  
Penaeus Vannamei ◽  
Dot Blot ◽  
Disease Symptoms ◽  
Infectious Myonecrosis Virus ◽  
Blot Hybridisation ◽  
Polymerase Chain ◽  
Shrimp Farms

Pacific white shrimp Penaeus vannamei Boone, 1931, is a recently introduced species in India. P. vannamei samples, collected from various shrimp farms of Karnataka, India were subjected to polymerase chain reaction (PCR) based detection of whitespot syndrome virus (WSSV), hepatopancreatic parvovirus (HPV), monodon baculovirus (MBV), infectious hypodermal and hematopoietic necrosis virus (IHHNV), taura syndrome virus (TSV) and infectious myonecrosis virus (IMNV). Out of the 81 shrimp samples analysed, 41 samples (50.6%) were found positive for WSSV and four (4.9%) were positive for IHHNV. Among 41 WSSV positive samples, 20 (48.7%) samples were found positive for WSSV by 1st step PCR, while the remaining 21 (51.2%) samples were positive by nested PCR. WSSV positive samples were further confirmed by dot blot hybridisation assay. However, clinical signs/disease symptoms were not observed in any of the shrimp samples tested positive for the viruses.

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