The Predictive Value of Ischemia-modified Albumin in Long-term Results of Ischemia-reperfusion Injury in an Experimental Testicular Torsion Model

Abstract Background Testicular torsion is a urological emergency in which misdiagnosis and inappropriate treatment can lead to testicular atrophy and male infertility owing to ischemia-reperfusion injury (IRI). Although experimental studies of testicular torsion have been preceded, promising therapeutic agents based on the long-term effect for spermatogenesis have not been identified in testicular ischemia reperfusion injury (IRI) animal model. Tadalafil, one of the phosphodiesterase-5 inhibitors commonly used in the treatment of erectile dysfunction, has recently reported a protective effect against IRI in several organs. In this study, we evaluated the long-term protective effect of tadalafil for spermatogenesis in a rat testicular IRI model. Methods Forty-eight adolescent Sprague–Dawley rats were divided into 6 groups (A-F). Sham operation was performed in group A. Group B received surgical 720-degree torsion of the left testis without any medication. Groups C, D, E, and F were operated surgical torsion with tadalafil at varying doses (0.3 mg/kg, 1.0 mg/kg) and durations (single or daily administration for 4 weeks). Detorsion was performed after 3 hour of torsion in all rats except the sham group. Four weeks after operation, both testes were evaluated of spermatogenesis using Johnsen scoring. To evaluate the protective effect of tadalafil against oxidative stress by IRI, malondialdehyde (MDA) and superoxide dismutase (SOD) level were analyzed via ELISA in both testes 4 hour after detorsion in the same experiments as in group A, B, and C. Results For the evaluation of spermatogenesis according to doses, the groups with high-dose tadalafil showed a higher Johnsen scores than low-dose counterparts. The groups with daily administration for 4weeks were observed a higher Johnsen scores than those given a single administration. Furthermore, molecular markers (MDA and SOD) related with oxidative stress and histopathologic findings showed remarkable improvement after tadalafil administration. Conclusion Tadalafil alleviated long-term deterioration of spermatogenesis and oxidative stress by restoring antioxidant status after testicular IRI rat model. Furthermore, it demonstrated a protective effect against testicular IRI in a time- and dose-dependent manner.

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1021: Selective Nuclear Factor Kappa B(NFκB) Inhibitor, Pyrolidium Dithiocarbamate Prevents, Long-Term Histologic Damage in Ischemia-Reperfusion Injury after Delayed Testicular Torsion

The Journal of Urology ◽

10.1016/s0022-5347(18)33246-4 ◽

2006 ◽

Vol 175 (4S) ◽

pp. 329-329

Author(s):

Sinan L. Kirecci ◽

Emin Ozbek ◽

Alper Otunctemur ◽

Levent Ozcan ◽

Adnan Somay ◽

...

Keyword(s):

Reperfusion Injury ◽

Testicular Torsion ◽

Nuclear Factor Kappa B ◽

Ischemia Reperfusion Injury ◽

Ischemia Reperfusion ◽

Nuclear Factor ◽

Nuclear Factor Kappa

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SP784RENAL TRANSPLANT ISCHEMIA/REPERFUSION INJURY CORRECTION WITH CYTOKINES ADSORPTION: EARLY AND LONG-TERM RESULTS

Nephrology Dialysis Transplantation ◽

10.1093/ndt/gfx158.sp784 ◽

2017 ◽

Vol 32 (suppl_3) ◽

pp. iii408-iii408

Author(s):

Aleksei Zulkarnaev ◽

Andrey Vatazin

Keyword(s):

Reperfusion Injury ◽

Ischemia Reperfusion Injury ◽

Ischemia Reperfusion ◽

Long Term Results

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The Predictive Value of Ischemia-Modified Albumin in Renal Ischemia-Reperfusion Injury

Urologia Internationalis ◽

10.1159/000500929 ◽

2019 ◽

Vol 103 (4) ◽

pp. 473-481 ◽

Cited By ~ 1

Author(s):

Huseyin Aytac Ates ◽

Uğur Yücetaş ◽

Erkan Erkan ◽

Esma Yucetas ◽

Soner Ulusoy ◽

...

Keyword(s):

Reperfusion Injury ◽

Predictive Value ◽

Ischemia Reperfusion Injury ◽

Ischemia Reperfusion ◽

Renal Ischemia ◽

Ischemia Modified Albumin ◽

Renal Ischemia Reperfusion Injury

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Histological Study on the Possible Ameliorating Effect of Platelet Rich Plasma on Ischemia/Reperfusion Injury in Testicular Torsion Model in Adult Albino Rat

Egyptian Journal of Histology ◽

10.21608/ejh.2019.9860.1091 ◽

2020 ◽

Vol 43 (2) ◽

pp. 614-629

Author(s):

Maha Gazia

Keyword(s):

Reperfusion Injury ◽

Testicular Torsion ◽

Ischemia Reperfusion Injury ◽

Platelet Rich Plasma ◽

Ischemia Reperfusion ◽

Histological Study ◽

Albino Rat

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Influence of long-term treatment with glyceryl trinitrate on remote ischemic conditioning

AJP Heart and Circulatory Physiology ◽

10.1152/ajpheart.00114.2018 ◽

2018 ◽

Vol 315 (1) ◽

pp. H150-H158 ◽

Cited By ~ 16

Author(s):

Marie Hauerslev ◽

Sivagowry Rasalingam Mørk ◽

Kasper Pryds ◽

Hussain Contractor ◽

Jan Hansen ◽

...

Keyword(s):

Endothelial Function ◽

Glyceryl Trinitrate ◽

Reperfusion Injury ◽

Ischemia Reperfusion Injury ◽

Ischemia Reperfusion ◽

Rat Myocardium ◽

Remote Ischemic Conditioning ◽

Ischemic Conditioning ◽

Human Endothelium

Remote ischemic conditioning (RIC) protects against sustained myocardial ischemia. Because of overlapping mechanisms, this protection may be altered by glyceryl trinitrate (GTN), which is commonly used in the treatment of patients with chronic ischemic heart disease. We investigated whether long-term GTN treatment modifies the protection by RIC in the rat myocardium and human endothelium. We studied infarct size (IS) in rat hearts subjected to global ischemia-reperfusion (I/R) in vitro and endothelial function in healthy volunteers subjected to I/R of the upper arm. In addition to allocated treatment, rats were coadministered with reactive oxygen species (ROS) or nitric oxide (NO) scavengers. Rats and humans were randomized to 1) control, 2) RIC, 3) GTN, and 4) GTN + RIC. In protocols 3 and 4, rats and humans underwent long-term GTN treatment for 7 consecutive days, applied subcutaneously or 2 h daily transdermally. In rats, RIC and long-term GTN treatment reduced mean IS (18 ± 12%, P = 0.007 and 15 ± 5%, P = 0.002) compared with control (35 ± 13%). RIC and long-term GTN treatment in combination did not reduce IS (29 ± 12%, P = 0.55 vs. control). ROS and NO scavengers both attenuated IS reduction by RIC and long-term GTN treatment. In humans, I/R reduced endothelial function ( P = 0.01 vs. baseline). Separately, RIC and long-term GTN prevented the reduction in endothelial function caused by I/R; given in combination, prevention was lost. RIC and long-term GTN treatment both protect against rat myocardial and human endothelial I/R injury through ROS and NO-dependent mechanisms. However, when given in combination, RIC and long-term GTN treatment fail to confer protection. NEW & NOTEWORTHY Remote ischemic conditioning (RIC) and long-term glyceryl trinitrate (GTN) treatment protect against ischemia-reperfusion injury in both human endothelium and rat myocardium. However, combined application of RIC and long-term GTN treatment abolishes the individual protective effects of RIC and GTN treatment on ischemia-reperfusion injury, suggesting an interaction of clinical importance.

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