Immunization of pigs with a type 2 modified live PRRSV vaccine prevents the development of a deadly long lasting hyperpyrexia in a challenge study with highly pathogenic PRRSV JX143

Vaccine ◽  
2013 ◽  
Vol 31 (16) ◽  
pp. 2062-2066 ◽  
Author(s):  
Zuzhang Wei ◽  
Jianwu Zhang ◽  
Jinshan Zhuang ◽  
Zhi Sun ◽  
Fei Gao ◽  
...  
Keyword(s):  
Highly Pathogenic ◽  
Challenge Study ◽  
Highly Pathogenic Prrsv

A novel HRM assay for differentiating classical strains and highly pathogenic strains of type 2 porcine reproductive and respiratory syndrome virus

2018 ◽  
Vol 39 ◽  
pp. 25-32 ◽  
Author(s):  
Junying Sun ◽  
Gali Bingga ◽  
Zhicheng Liu ◽  
Chunhong Zhang ◽  
Haiyan Shen ◽  
...  
Keyword(s):  
Respiratory Syndrome Virus ◽  
Highly Pathogenic

Nanoparticle Vaccine for Avian Influenza Virus: A Challenge Study against Highly Pathogenic H5N2 Subtype

2018 ◽  
Vol 07 (01) ◽  
Author(s):  
Jianping Li ◽  
Zeinab Helal ◽  
Brian Ladman ◽  
Christopher Karch ◽  
Jack Gelb Jr ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Avian Influenza ◽  
Avian Influenza Virus ◽  
Highly Pathogenic ◽  
Influenza Virus A ◽  
Challenge Study

scholarly journals The 30-Amino-Acid Deletion in the Nsp2 of Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus Emerging in China Is Not Related to Its Virulence

2009 ◽  
Vol 83 (10) ◽  
pp. 5156-5167 ◽  
Author(s):  
Lei Zhou ◽  
Jialong Zhang ◽  
Jingwen Zeng ◽  
Shuoyan Yin ◽  
Yanhua Li ◽  
...  
Keyword(s):  
Amino Acid ◽  
Parental Virus ◽  
Respiratory Syndrome Virus ◽  
Cdna Clones ◽  
Coding Region ◽  
Amino Acid Deletion ◽  
Highly Pathogenic ◽  
Specific Pathogen ◽  
Prrsv Strain ◽  
Highly Pathogenic Prrsv

ABSTRACT During the past 2 years, an atypical clinical outbreak, caused by a highly pathogenic porcine reproductive and respiratory syndrome virus (PRRSV) with a unique 30-amino-acid deletion in its Nsp2-coding region, was pandemic in China. In this study, we generated four full-length infectious cDNA clones: a clone of the highly virulent PRRSV strain JXwn06 (pWSK-JXwn), a clone of the low-virulence PRRSV strain HB-1/3.9 (pWSK-HB-1/3.9), a chimeric clone in which the Nsp2 region containing the 30-amino-acid deletion was replaced by the corresponding region of the low-virulence PRRSV strain HB-1/3.9 (pWSK-JXwn-HB1nsp2), and a mutated HB-1/3.9 clone with the same deletion in Nsp2 as JXwn06 (pWSK-HB1-ND30). We also investigated the pathogenicities of the rescued viruses (designated RvJXwn, RvJXwn-HB1nsp2, RvHB-1/3.9, and RvHB1-ND30, respectively) in specific-pathogen-free piglets in order to determine the role of the 30-amino-acid deletion in the virulence of the highly pathogenic PRRSV. All the rescued viruses could replicate stably in MARC-145 cells. Our findings indicated that RvJXwn-HB1nsp2 retained high virulence for piglets, like RvJXwn and the parental virus JXwn06, although the survival time of piglets infected with RvJXwn-HB1nsp2 was obviously prolonged. RvHB1-ND30 exhibited low virulence for piglets, like RvHB-1/3.9 and the parental virus HB-1/3.9. Therefore, we conclude that the 30-amino-acid deletion is not related to the virulence of the highly pathogenic PRRSV emerging in China.

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