Immune responses to modified live virus vaccines developed from classical or highly pathogenic PRRSV following challenge with a highly pathogenic PRRSV strain

2016 ◽  
Vol 62 ◽  
pp. 1-7 ◽  
Author(s):  
Gang Wang ◽  
Ying Yu ◽  
Chong Zhang ◽  
Yabin Tu ◽  
Jie Tong ◽  
...  
Keyword(s):  
Immune Responses ◽  
Highly Pathogenic ◽  
Live Virus ◽  
Prrsv Strain ◽  
Highly Pathogenic Prrsv

scholarly journals The 30-Amino-Acid Deletion in the Nsp2 of Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus Emerging in China Is Not Related to Its Virulence

2009 ◽  
Vol 83 (10) ◽  
pp. 5156-5167 ◽  
Author(s):  
Lei Zhou ◽  
Jialong Zhang ◽  
Jingwen Zeng ◽  
Shuoyan Yin ◽  
Yanhua Li ◽  
...  
Keyword(s):  
Amino Acid ◽  
Parental Virus ◽  
Respiratory Syndrome Virus ◽  
Cdna Clones ◽  
Coding Region ◽  
Amino Acid Deletion ◽  
Highly Pathogenic ◽  
Specific Pathogen ◽  
Prrsv Strain ◽  
Highly Pathogenic Prrsv

ABSTRACT During the past 2 years, an atypical clinical outbreak, caused by a highly pathogenic porcine reproductive and respiratory syndrome virus (PRRSV) with a unique 30-amino-acid deletion in its Nsp2-coding region, was pandemic in China. In this study, we generated four full-length infectious cDNA clones: a clone of the highly virulent PRRSV strain JXwn06 (pWSK-JXwn), a clone of the low-virulence PRRSV strain HB-1/3.9 (pWSK-HB-1/3.9), a chimeric clone in which the Nsp2 region containing the 30-amino-acid deletion was replaced by the corresponding region of the low-virulence PRRSV strain HB-1/3.9 (pWSK-JXwn-HB1nsp2), and a mutated HB-1/3.9 clone with the same deletion in Nsp2 as JXwn06 (pWSK-HB1-ND30). We also investigated the pathogenicities of the rescued viruses (designated RvJXwn, RvJXwn-HB1nsp2, RvHB-1/3.9, and RvHB1-ND30, respectively) in specific-pathogen-free piglets in order to determine the role of the 30-amino-acid deletion in the virulence of the highly pathogenic PRRSV. All the rescued viruses could replicate stably in MARC-145 cells. Our findings indicated that RvJXwn-HB1nsp2 retained high virulence for piglets, like RvJXwn and the parental virus JXwn06, although the survival time of piglets infected with RvJXwn-HB1nsp2 was obviously prolonged. RvHB1-ND30 exhibited low virulence for piglets, like RvHB-1/3.9 and the parental virus HB-1/3.9. Therefore, we conclude that the 30-amino-acid deletion is not related to the virulence of the highly pathogenic PRRSV emerging in China.

scholarly journals Evaluation of the Efficacy of an Attenuated Live Vaccine against Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus in Young Pigs

2012 ◽  
Vol 19 (8) ◽  
pp. 1199-1206 ◽  
Author(s):  
Xue Leng ◽  
Zhenguang Li ◽  
Mingqi Xia ◽  
Yanliang He ◽  
Hua Wu
Keyword(s):  
Respiratory Syndrome Virus ◽  
Effective Protection ◽  
Lethal Challenge ◽  
Highly Pathogenic ◽  
Live Virus ◽  
Pork Industry ◽  
Live Virus Vaccine ◽  
Young Pigs ◽  
Prrsv Strain

ABSTRACTHighly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) is characterized by high fever and high mortality in pigs of all ages and has severely affected the pork industry of China in the last few years. An attenuated HP-PRRSV strain, TJM, was obtained by passaging HP-PRRSV strain TJ on MARC-145 cells for 92 passages. Porcine reproductive and respiratory syndrome virus (PRRSV)- and antibody-free pigs were inoculated intramuscularly with TJM (105.050% tissue culture infective doses [TCID50]) and challenged at 28, 60, 120, and 180 days postimmunization (dpi). The results showed that 5/5, 5/5, 5/5, and 4/5 immunized pigs were protected from the lethal challenge and did not develop fever and clinical diseases at each challenge, respectively. Compared to control pigs, vaccinated pigs showed much milder pathological lesions and gained significantly more weight (P< 0.01). Sequence analysis of different passages of strain TJ showed that the attenuation resulted in a deletion of a continuous 120 amino acids (aa), in addition to the discontinuous 30-aa deletion in the nsp2 region. The analysis also demonstrated that the 120-aa deletion was genetically stablein vivo. These results suggested that HP-PRRSV TJM was efficacious against a lethal challenge with a virulent HP-PRRSV strain, and effective protection could last at least 4 months. Therefore, strain TJM is a good candidate for an efficacious modified live virus vaccine as well as a useful molecular marker vaccine against HP-PRRSV.

scholarly journals An infectious cDNA clone of a highly pathogenic porcine reproductive and respiratory syndrome virus variant associated with porcine high fever syndrome

2008 ◽  
Vol 89 (9) ◽  
pp. 2075-2079 ◽  
Author(s):  
Jian Lv ◽  
Jianwu Zhang ◽  
Zhi Sun ◽  
Weiquan Liu ◽  
Shishan Yuan
Keyword(s):  
Clinical Symptoms ◽  
Animal Experiments ◽  
High Fever ◽  
Respiratory Syndrome Virus ◽  
Cdna Clones ◽  
Highly Pathogenic ◽  
Virus Variant ◽  
Recombinant Viruses ◽  
Prrsv Strain ◽  
Highly Pathogenic Prrsv

Since May 2006, a so-called ‘porcine high fever syndrome’ (PHFS) has spread all over China. The arterivirus porcine reproductive and respiratory syndrome virus (PRRSV) was believed to be the main causative agent, although the involvement of other pathogens was not formally excluded. The genome of a representative Chinese PRRSV strain, named JX143, was sequenced and used to develop infectious cDNA clones, pJX143 and pJX143M, with the latter containing an engineered MluI site that served as a genetic marker. In various virological assays, the rescued viruses, vJX143 and vJX143M, were indistinguishable from their parental virus. Animal experiments showed that these recombinant viruses retained the high pathogenicity and induced the typical clinical symptoms observed during PHFS outbreaks. This is the first report describing infectious cDNA clones of this highly pathogenic PRRSV. Our results unambiguously fulfil Koch's postulates and define highly pathogenic PRRSV as the aetiological agent of PHFS in China.

scholarly journals Dynamic Changes in Inflammatory Cytokines in Pigs Infected with Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus

2010 ◽  
Vol 17 (9) ◽  
pp. 1439-1445 ◽  
Author(s):  
Yonggang Liu ◽  
Wenda Shi ◽  
Enmin Zhou ◽  
Shujie Wang ◽  
Shouping Hu ◽  
...  
Keyword(s):  
Immune Responses ◽  
Inflammatory Cytokines ◽  
Peripheral Blood ◽  
Interleukin 1 ◽  
Respiratory Syndrome Virus ◽  
Necrosis Factor Alpha ◽  
Highly Pathogenic ◽  
Cytokine Levels ◽  
Loss Of Appetite ◽  
Highly Pathogenic Prrsv

ABSTRACT Porcine reproductive and respiratory syndrome virus (PRRSV) infection induces both humoral and cellular immune responses. In this study, we investigated the changes in cytokine levels in peripheral blood between the highly pathogenic PRRSV HuN4 strain and its derivative strain HuN4-F112 obtained by serial propagation in MARC145 cells to 112 passages. The results demonstrated that pigs infected with HuN4 showed a loss of appetite, decrease in body weight, raised body temperature, and respiratory symptoms, along with interstitial pneumonia lesions. The PRRSV amounts in the pigs infected with HuN4 were 105 to 109 copies/ml in the blood and 1010 to 1011 copies/g in the lung tissues, whereas the virus amounts with HuN4-F112 were 102.15 to 103.13 copies/ml in the blood and 103.0 to 103.6 copies/g in the lungs. Moreover, the levels of interleukin 1 (IL-1), IL-6, tumor necrosis factor alpha (TNF-α), and alpha interferon (IFN-α) in peripheral blood were upregulated 7 days postinoculation with HuN4, which was earlier than in the HuN4-F112 group. Furthermore, cytokine levels in the pigs infected with HuN4 returned to normal on the 21st day postinoculation, while the levels in those infected with HuN4-F112 continued to increase. These results demonstrated that the pigs infected with the highly pathogenic PRRSV HuN4 strain generated earlier and higher levels of inflammatory cytokines, and the results also indicated that HuN4 may aggravate inflammation and damage tissues and organs. The low-pathogenic PRRSV HuN4-F112 strain induced lower levels of inflammatory cytokines, which may enhance the immune responses against the infection.

scholarly journals Complete Genome Sequence of a Mosaic NADC30-Like Porcine Reproductive and Respiratory Syndrome Virus in China

2016 ◽  
Vol 4 (6) ◽  
Author(s):  
Lin-jian Wang ◽  
Zhenhua Guo ◽  
Songlin Qiao ◽  
Xin-xin Chen ◽  
Gaiping Zhang
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Henan Province ◽  
Respiratory Syndrome Virus ◽  
Highly Pathogenic ◽  
Prrsv Strain ◽  
Highly Pathogenic Prrsv

Here, we report the complete genome of an NADC30-like porcine reproductive and respiratory syndrome virus (PRRSV) strain, HNhx, which was isolated from Henan Province, China, in 2016 and was characterized by recombination with JXA1 strain (an epidemic highly pathogenic PRRSV strain in China) in Nsp4 to Nsp9.

scholarly journals Comparison of Immune Responses in Pigs Infected with Chinese Highly Pathogenic PRRS Virus Strain HV and North American Strain NADC-20

2017 ◽  
Vol 11 (1) ◽  
pp. 73-82 ◽  
Author(s):  
X. Li ◽  
A. Galliher-Beckley ◽  
L. Wang ◽  
J. Nietfeld ◽  
W. Feng ◽  
...  
Keyword(s):  
Immune Responses ◽  
North American ◽  
High Morbidity ◽  
Flow Cytometry Analysis ◽  
Highly Pathogenic ◽  
Prrs Virus ◽  
Tnf Α ◽  
American Strain ◽  
Prrsv Strain ◽  
Ifn Γ

Introduction:Chinese HP-PRRSV characterized by high morbidity and mortality of all ages of pigs emerged since 2006 in China. The immune response of HP-PRRSV was never compared with conventional low pathogenic PRRSV strain.Objective:In this study, we compared the immune responses elicited by a Chinese HP-PRRSV strain HV and a North American RRSV strain NADC20 infections.Result:Pigs infected with NADC-20 showed significantly higher Ab titers than HV-PRRSV infected pigs at 9 DPI. Infection with HV-PRRSV induced a significantly higher levels of TNF-α and IL-10 in both sera and lung tissues and higher IFN-α and IFN-γ in the serum. Flow cytometry analysis showed that HV-PRRSV infected pigs generated significantly higher frequencies of NK cells in the peripheral blood and Th/memory, CTLs, and T-reg cells in the lung as compared with NADC-20 infected pigs.Conclusion:This study demonstrates that different immunity profiles were elicited by HV-PRRSV and NADC-20, and these differences may contribute to the distinct pathogenesis of HV-PRRSV and NADC-20.

scholarly journals Complete Genome Sequence of a Recombinant NADC30-Like Strain, SCnj16, of Porcine Reproductive and Respiratory Syndrome Virus in Southwestern China

2018 ◽  
Vol 6 (6) ◽  
pp. e00004-18 ◽  
Author(s):  
Long Zhou ◽  
Runmin Kang ◽  
Bo Xie ◽  
Yiming Tian ◽  
Xin Yang ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Nonstructural Protein ◽  
Respiratory Syndrome Virus ◽  
Amino Acid Deletion ◽  
Highly Pathogenic ◽  
Nonstructural Protein 2 ◽  
Prrsv Strain ◽  
Highly Pathogenic Prrsv

ABSTRACTThe NADC30-like strains of porcine reproductive and respiratory syndrome virus (PRRSV) are characterized by a 131-amino-acid deletion in nonstructural protein 2 (NSP2). Here, we report the complete genome sequence of a recombinant NADC30-like PRRSV strain, SCnj16, that exhibits the molecular marker of the Chinese highly pathogenic PRRSV (HP-PRRSV) in NSP2.

scholarly journals Recombinant proteins of Zaire ebolavirus induce potent humoral and cellular immune responses and protect against live virus infection in mice

Vaccine ◽  
2018 ◽  
Vol 36 (22) ◽  
pp. 3090-3100 ◽  
Author(s):  
Axel T. Lehrer ◽  
Teri-Ann S. Wong ◽  
Michael M. Lieberman ◽  
Tom Humphreys ◽  
David E. Clements ◽  
...  
Keyword(s):  
Virus Infection ◽  
Immune Responses ◽  
Recombinant Proteins ◽  
Live Virus ◽  
Cellular Immune Responses ◽  
Zaire Ebolavirus ◽  
Cellular Immune

scholarly journals Complete Genome Sequence of a Recombinant Porcine Reproductive and Respiratory Syndrome Virus Strain from Two Genotype 1 Modified Live Virus Vaccine Strains

2017 ◽  
Vol 5 (22) ◽  
Author(s):  
Patricia Renson ◽  
Fabrice Touzain ◽  
Arnaud Lebret ◽  
Mireille Le Dimna ◽  
Hélène Quenault ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Virus Vaccine ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Respiratory Syndrome Virus ◽  
Genotype 1 ◽  
Live Virus ◽  
Live Virus Vaccine ◽  
Pig Farm ◽  
Prrsv Strain

ABSTRACT This paper provides information on the complete genome sequence of a porcine reproductive and respiratory syndrome virus (PRRSV) strain isolated on a French pig farm which was identified as a recombinant strain from two commercial modified live virus vaccine strains of genotype 1 (VP-046BIS and DV strains).

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