Transcriptome profiling in blood before and after hepatitis B vaccination shows significant differences in gene expression between responders and non-responders

AbstractFusarium wilt, caused by the fungus Fusarium oxysporum Schlecht. f. sp. vasinfectum (Fov) is a destructive soil-borne cotton disease. To profile the genes and pathways responding to Fov infection, we compared transcriptomic responses before and after F. oxysporum inoculation in a highly resistant cotton cultivar, Yumian21, and a highly susceptible cultivar, Jimian11. Although the overall gene expression pattern was downregulated in both cultivars, the global gene expression in the resistant cultivar was stronger than that in the susceptible cultivar. In addition, the expressed genes of two cultivars mostly differed in “cellular process,” “single-organism process,” “metabolic process,” and “response to stimulus” functional groups in the biological process Gene Ontology category: the upregulated differentially expressed genes (DEG) were largely enriched in the resistant cultivar, while the downregulated DEGs were largely enriched in the susceptible cultivar. Phenylpropanoid biosynthesis and phenylalanine metabolism are the key metabolic pathways in cotton in response to Fov. We found that lignin plays a potential role in cotton resistance to Fov. Two coding genes, caffeic acid 3-O-methyltransferase and peroxidase2, as well as the two transcription factors MYB46 and MYB86, are possibly involved in the accumulation and synthesis of lignin. Furthermore, the result showed that the quantification of lignin could be potentially used as a selection tool to identify Fusarium wilt resistant cotton.

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An audit of hepatitis B vaccination for men who have sex with men before and after sexual health service integration

International Journal of STD & AIDS ◽

10.1177/0956462415626307 ◽

2016 ◽

Vol 27 (10) ◽

pp. 898-900 ◽

Cited By ~ 4

Author(s):

Sally Wielding ◽

Andy HY Ma ◽

Daniel J Clutterbuck

Keyword(s):

Health Service ◽

Hepatitis B ◽

Sexual Health ◽

Service Integration ◽

Hepatitis B Vaccination ◽

Sexual Health Service ◽

Before And After ◽

Sex With Men

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P108 How do men who have sex with men fare in integrated sexual health centres? an audit of hepatitis b vaccination rates before and after integration

Sexually Transmitted Infections ◽

10.1136/sextrans-2015-052126.151 ◽

2015 ◽

Vol 91 (Suppl 1) ◽

pp. A51.3-A52

Author(s):

Sally Wielding ◽

Andy Ma ◽

Dan Clutterbuck

Keyword(s):

Hepatitis B ◽

Sexual Health ◽

Hepatitis B Vaccination ◽

Vaccination Rates ◽

Health Centres ◽

Before And After ◽

Sex With Men

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Comparative Analysis of Hepatitis B Virus Infections in Blood Donors Born Before and After the I Mplementation of Universal HBV Vaccination in Southern China

10.21203/rs.3.rs-101501/v1 ◽

2020 ◽

Author(s):

Xianlin Ye ◽

Tong Li ◽

Yi Li ◽

Jinfeng Zeng ◽

Ran Li ◽

...

Keyword(s):

Hepatitis B ◽

Blood Donors ◽

Vaccination Program ◽

Escape Mutation ◽

Hepatitis B Vaccination ◽

Infected Blood ◽

Hbv Vaccination ◽

Significant Difference ◽

Before And After ◽

Genotype C

Abstract BackgroundNeonatal hepatitis B vaccination program at birth has been implemented nationwide since 1992 in China, which may impact HBV safety in blood donations actively. The true prevalence of HBV, HBsAg, and OBI between vaccinated blood donors and non-vaccinated blood donors should be explored. Study design and methodsThe samples of blood donors were collected and detected for serologic markers of HBV in the Shenzhen Blood Center between Feb 2016 and Jun 2016. The discrepant results were tested with commercial electrochemiluminescence immunoassay (ELCI), alternative MPX ID NAT, nested PCR, sequencing, and a quantitative real-time polymerase chain reaction (PCR) assay. HBsAg and anti-HBs were quantified. The serological and molecular characteristics of HBV infected blood donors were analyzed, and the effects on blood safety for donors born before and after the implementation of universal HBV vaccination were compared. ResultsTotal of 242 reactive by NAT and/or HBsAg ELISA samples from 26318 candidate donors, 192 (0.73%, [95%CI, 0.63-0.84]) HBV+, 131 (0.49%, [95%CI, 0.43-0.59]) HBsAg+, 58 (0.22%, [95%CI,0.17-0.28]) OBI were confirmed respectively. The HBV+ rate in vaccinated donors is lower than in non-vaccinated donors (P<0.05). The HBsAg titers of vaccinated infected blood donors are much higher than non vaccinated infected blood donors. The OBI yield rates in the vaccinated blood donors were 0.11% (7/6422), and significantly lower than 0.26% (51/19898) in the non vaccinated blood donors (P<0.05). 102/124 (82.3%) samples are genotype B, 22/124 (17.7%) are genotype C total. There is no significant difference in the distribution of genotype in the non vaccinated blood donors (B/C,86/17) and the vaccinated blood donors (B/C,23/6) (P>0.05). High frequency of vaccine escape mutation M133L (32.4%) and E164G in S region of genotype B strains and substitution L175S (40.9%) related to vaccine escape in S region of genotype C strains identified.ConclusionThe universal HBV vaccination program markedly reduces the risk of HBV infection in blood donors, and provides a significant guarantee for the safety of blood transfusion. Several important mutations detected related vaccine escape and notable mutations needed further investigated.

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