Timing as a mechanism of development and evolution in the cerebral cortex

One of the biggest mysteries in neurobiology concerns the mechanisms responsible for the diversification of the brain over different time scales i.e. during development and evolution. Subtle differences in the timing of biological processes during development, e.g. onset, offset, duration, speed and sequence, can trigger large changes in phenotypic outcomes. At the level of a single organism, altered timing of developmental events can lead to individual variability, as well as malformation and disease. At the level of phylogeny, there are known interspecies differences in the timing of developmental events, and this is thought to be an important factor that drives phenotypic variation across evolution, known as heterochrony. A particularly striking example of phenotypic variation is the evolution of human cognitive abilities, which has largely been attributed to the development of the mammalian-specific neocortex and its subsequent expansion in higher primates. Here, I review how the timing of different aspects of cortical development specifies developmental outcomes within species, including processes of cell proliferation and differentiation, neuronal migration and lamination, and axonal targeting and circuit maturation. Some examples of the ways that different processes might “keep time” in the cortex are explored, reviewing potential cell-intrinsic and -extrinsic mechanisms. Further, by combining this knowledge with known differences in timing across species, timing changes that may have occurred during evolution are identified, which perhaps drove the phylogenetic diversification of neocortical structure and function.

PHYSICAL TRAINING FOR 7-9 YEAR OLD JUDOISTS

Judo is a detailed system of movements that allows you to govern the body as a harmoniously single organism capable of developing the physical abilities and functional fitness of athletes. In the conditions of competitive activity in judo the individual multivariate way of technical and tactical performance in the form of the throws, restraints, painful and suffocating locks is observed. Performing all these techniques requires strength, endurance, instant speed and coordination skills. The purpose of the article is improvement the methods of general and special physical training development for 7-9 year-old judokas. The experimentation facility was Children's and Youth Sports School № 11, Zaporizhia. It was attended by 43 boys aged 7-9 who were divided into control (n=21) and experimental (n=22) groups, all boys were classified in the main medical group. All sportsmen were in the primary training stage. The research paper describes the improved method of 7-9 year-old judokas training. The method determines and theoretically justifies correspondence of physical exercises and proportionality of muscle loading with functional peculiarities for child’s body. The method is based on multifaceted special preparation that helps to broaden motion behavior experience of young judokas and create appropriate base for their general technical preparedness. The efficiency of the developed method was investigated when comparing the obtained indicators in the control and experimental groups. The percentage increase in indicators was divided into three groups: the smallest, average and highest. But in the experimental group all indicators improved, in the control group - some of them improved slightly, others did not undergo significant changes. In accordance to the research, there is a positive influence of the improved methodology on the development of general and special physical preparedness level among 7-9 year-old judokas.

First comprehensive proteome analysis of lysine crotonylation in Streptococcus agalactiae, a pathogen causing meningoencephalitis in teleosts

Backgroud Streptococcus agalactiae is a common colonizer of the rectovaginal tract and lead to infectious diseases of neonatal and non-pregnant adults, which also causes infectious disease in fish and a zoonotic risk as well. Lysine crotonylation (Kcr) is a kind of histone post-translational modifications discovered in 2011. In yeast and mammals, Kcr function as potential enhancers and promote gene expression. However, lysine crotonylation in S. agalactiae has not been studied yet. Methods In this study, the crotonylation profiling of fish pathogen, S. agalactiae was investigated by combining affinity enrichment with LC MS/MS. The Kcr modification of several selected proteins were further validated by Western blotting. Results In the present study, we conducted the proteome-wide profiling of Kcr in S. agalactiae and identified 241 Kcr sites from 675 screened proteins for the first time. Bioinformatics analysis showed that 164 sequences were matched to a total of six definitively conserved motifs, and many of them were significantly enriched in metabolic processes, cellular process, and single-organism processes. Moreover, four crotonylation modified proteins were predicted as virulence factors or to being part of the quorum sensing system PTMs on bacteria. The data are available via ProteomeXchange with identifier PXD026445. Conclusions These data provide a promising starting point for further functional research of crotonylation in bacterial virulence in S. agalactiae.

A.S. Khomyakov and “people’s thought” in the epic novel by Leo Tolstoy “War and Peace”

The article reveals the deep connections of the “people’s thought” and Tolstoy’s philosophy of history in “War and Peace” with the theological and literary-critical works of A.S. Khomyakova. The author of the work analyzes the dispute between Tolstoy and the cult of an outstanding personality, with the Hegelian understanding of his role in the historical process. Tolstoy is alien to the Hegelian rise of “great personalities” over the masses, the Hegelian liberation of the “genius” from moral control and evaluation. Tolstoy believes that it is not an exceptional personality, but the life of the people that turns out to be the most sensitive organism, catching the will of Providence, intuitively sensing the hidden meaning of the historical movement. Anticipating Tolstoy, Khomyakov sharply criticizes the cult of personality in the church hierarchy, the Catholic dogma of papal infallibility, of the unconditional authority of an individual in matters of conscience and faith. Khomyakov reveals deep religious roots that feed the centuries-old Western enmity towards Russia. The article proves that Tolstoy is close to Khomyakov’s idea that Divine Providence overshadows with its grace only the believing people, united into a single organism by Christian love, that the epic basis of “War and Peace” is anticipated in Khomyakov’s literary-critical works “Glinka’s Opera ‘Life for Tsar’”, “On the Possibility of the Russian Art School”, “Ivanov’s Painting. Letter to the editor of ‘Russian Beseda’”. The article proves that “War and Peace” overcomes the conflict between the individual and society, the hero and the people, and reveals the epic horizons lost in the Western European novel.

Presence of Candida tropicalis on Staphylococcus epidermidis Biofilms Facilitated Biofilm Production and Candida Dissemination: An Impact of Fungi on Bacterial Biofilms

While Staphylococcus epidermidis (SE) is a common cause of infections in implanted prostheses and other indwelling devices, partly due to the biofilm formation, Candida tropicalis (CT) is an emerging Candida spp. with a potent biofilm-producing property. Due to the possible coexistence between SE and CT infection in the same patient, characteristics of the polymicrobial biofilms from both organisms might be different from those of the biofilms of each organism. Then, the exploration on biofilms, from SE with or without CT, and an evaluation on l-cysteine (an antibiofilm against both bacteria and fungi) were performed. As such, Candida incubation in preformed SE biofilms (SE > CT) produced higher biofilms than the single- (SE or CT) or mixed-organism (SE + CT) biofilms as determined by crystal violet staining and fluorescent confocal images with z-stack thickness analysis. In parallel, SE > CT biofilms demonstrated higher expression of icaB and icaC than other groups at 20 and 24 h of incubation, suggesting an enhanced matrix polymerization and transportation, respectively. Although organism burdens (culture method) from single-microbial biofilms (SE or CT) were higher than multi-organism biofilms (SE + CT and SE > CT), macrophage cytokine responses (TNF-α and IL-6) against SE > CT biofilms were higher than those in other groups in parallel to the profound biofilms in SE > CT. Additionally, sepsis severity in mice with subcutaneously implanted SE > CT catheters was more severe than in other groups as indicated by mortality rate, fungemia, serum cytokines (TNF-α and IL-6), and kidney and liver injury. Although CT grows upon preformed SE-biofilm production, the biofilm structures interfered during CT morphogenesis leading to the frailty of biofilm structure and resulting in the prominent candidemia. However, l-cysteine incubation together with the organisms in catheters reduced biofilms, microbial burdens, macrophage responses, and sepsis severity. In conclusion, SE > CT biofilms prominently induced biofilm matrix, fungemia, macrophage responses, and sepsis severity, whereas the microbial burdens were lower than in the single-organism biofilms. All biofilms were attenuated by l-cysteine.

First Comprehensive Proteome Analysis of Lysine Crotonylation in Streptococcus Agalactiae, A Pathogen Causing Meningoencephalitis in Teleosts

Backgroud Streptococcus agalactiae is a common colonizer of the rectovaginal tract and lead to infectious diseases of neonatal and non-pregnant adults, which also causes infectious disease in fish and a zoonotic risk as well. Lysine crotonylation (Kcr) is a kind of histone post-translational modifications discovered in 2011. In yeast and mammals, Kcr function as potential enhancers and promote gene expression. However, lysine crotonylation in S. agalactiae has not been studied yet. Methods In this study, the crotonylation profiling of fish pathogen, S. agalactiae was investigated by combining affinity enrichment with LC MS/MS. The Kcr modification of several selected proteins were further validated by Western blotting combined with real time PCR. Results In the present study, we conducted the proteome-wide profiling of Kcr in S. agalactiae and identified 241 Kcr sites from 675 screened proteins for the first time. Bioinformatics analysis showed that 164 sequences were matched to a total of six definitively conserved motifs, and many of them were significantly enriched in metabolic processes, cellular process, and single-organism processes. Moreover, four crotonylation modified proteins were predicted as virulence factors or to being part of the quorum sensing system PTMs on bacteria. The data are available via ProteomeXchange with identifier PXD026445. Conclusions These data provide a promising starting point for further functional research of crotonylation in bacterial virulence in S. agalactiae .

Humanism as the essence of the principle of religious tolerance (on the example of the Ideas of L. N. Tolstoy, F. M. Dostoevsky, and N. N. Pirogov)

During the period of globalization, various social strata comprise a new conceptual system. This ongoing transformational process prompts reconsideration of the fundamental concept of religious tolerance: it disintegrates and accretes with extraneous connotations. The worldwide growing religious fanaticism makes the problem of religious tolerance exceedingly acute. Its comprehension becomes relevant not only on the examples of countries, but also on the legacy of prominent representatives of different eras. The object of this research is the literary-philosophical heritage of L. N. Tolstoy, F. M. Dostoevsky, and N. N. Pirogov. The subject is the principle of religious tolerance in the worldview of the listed philosophers. Each of them demonstrates a superior example of humanism in the era of close interaction of various ethnic groups. The analysis of works of the three cultural figures indicates that despite the difference in worldviews, they all agreed upon the general humanistic essence of religious tolerance. The recognition of religious tolerance as a general cultural universal is based on their perception of the world as a single organism that does not unify various religious traditions, but rather constitute them into the “unity of diversified”. If L. N. Tolstoy elucidates humanistic nature of religious tolerance on the level of the individual, communities and entire humanity, then F. M. Dostoevsky has such reflections due to theme of war and peace. In combination with the “cosmic” worldview of N. N. Pirogov, these three views reveal different edges of religious tolerance based on the principle of complementarity. The study of humanistic ideas of the Russian philosophers may contribute to the creation of the methodological concept of religious tolerance as one of the fundamentals of the state domestic and foreign policy of the Russian Federation during the globalization era.

The Inquiline Ant Myrmica karavajevi Uses Both Chemical and Vibroacoustic Deception Mechanisms to Integrate into Its Host Colonies

Social parasitism represents a particular type of agonistic interaction in which a parasite exploits an entire society instead of a single organism. One fascinating form of social parasitism in ants is the “inquilinism”, in which a typically worker-less parasitic queen coexists with the resident queen in the host colony and produces sexual offspring. To bypass the recognition system of host colonies, inquilines have evolved a repertoire of deceiving strategies. We tested the level of integration of the inquiline Myrmica karavajevi within the host colonies of M. scabrinodis and we investigated the mechanisms of chemical and vibroacoustic deception used by the parasite. M. karavajevi is integrated into the ant colony to such an extent that, in rescue experiments, the parasite pupae were saved prior to the host’s brood. M. karavajevi gynes perfectly imitated the cuticular hydrocarbon profiles of M. scabrinodis queens and the parasite vibroacoustic signals resembled those emitted by the host queens eliciting the same levels of attention in the host workers during playback experiments. Our results suggest that M. karavajevi has evolved ultimate deception strategies to reach the highest social status in the colony hierarchy, encouraging the use of a combined molecular and behavioural approach when studying host–parasite interactions.

Homeostatic swimming of zooplankton upon crowding: the case of the copepod Centropages typicus

Crowding has a major impact on the dynamics of many material and biological systems, inducing effects as diverse as glassy dynamics and swarming. While this issue has been deeply investigated for a variety of living organisms, more research remains to be done on the effect of crowding on the behaviour of copepods, the most abundant metazoans on Earth. To this aim, we experimentally investigate the swimming behaviour, used as a dynamic proxy of animal adaptations, of males and females of the calanoid copepod Centropages typicus at different densities of individuals (10, 50 and 100 ind. l −1 ) by performing three-dimensional single-organism tracking. We find that the C. typicus motion is surprisingly unaffected by crowding over the investigated density range. Indeed, the mean square displacements as a function of time always show a crossover from ballistic to Fickian regime, with poor variations of the diffusion constant on increasing the density. Close to the crossover, the displacement distributions display exponential tails with a nearly density-independent decay length. The trajectory fractal dimension, D 3D ≅ 1.5, and the recently proposed ‘ecological temperature’ also remain stable on increasing the individual density. This suggests that, at least over the range of animal densities used, crowding does not impact on the characteristics of C. typicus swimming motion, and that a homeostatic mechanism preserves the stability of its swimming performance.