A genotype VII Newcastle disease virus-like particles confer full protection with reduced virus load and decreased virus shedding

Vaccine ◽  
2019 ◽  
Vol 37 (3) ◽  
pp. 444-451
Author(s):  
Xiaohong Xu ◽  
Zhuang Ding ◽  
Qianliang Yuan ◽  
Jiaxin Ding ◽  
Jindou Li ◽  
...  
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Virus Shedding ◽  
Virus Load ◽  
Virus Like Particles ◽  
Full Protection ◽  
Genotype Vii

EFFECT ON IMMUNE RESPONSE AND VIRUS SHEDDING IN THE CHICKEN VACCINATED AGAINST INACTIVATED LOCAL STRAIN OF NEWCASTLE DISEASE VIRUS GENOTYPE VII

2015 ◽  
Vol 77 (25) ◽  
Author(s):  
Nursyuwari Nayan ◽  
Syamsiah Aini Shohaimi ◽  
Raha Ahmad Raus ◽  
Afzan Mat Yusof ◽  
Ong Geok Huai
Keyword(s):  
Immune Response ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Local Strain ◽  
Control Group ◽  
Inactivated Vaccine ◽  
Virus Shedding ◽  
Oil Emulsion ◽  
Genotype Vii

The effectiveness of the new inactivated vaccine develop from local velogenic strain of Newcastle disease virus (NDV) Genotype VII and commercial vaccines LaSota were compared by determining the immune response and virus shedding of vaccinated chickens. Ten different groups of chicken consists of ten each, were vaccinated with and without adjuvant of the inactivated vaccine via intramuscular and subcutaneous, respectively. Three different adjuvants were used which include Oil-emulsion Complete Freund’s, Oil-emulsion Squalene-based, and aluminium hydroxide gel (Alum). As a comparison, a group of chicken was vaccinated with commercial vaccine and control group was not vaccinated. With 100% survival rate and highest mean haemaglutination-inhibition (HI) titre of log2 6, the inactivated vaccine with adjuvant Alum and Oil-emulsion Complete Freund’s surpassed the LaSota. In addition, the virus shedding was significantly reduced and comparable to LaSota vaccinated chicken. Whereas, without adjuvant, the chicken HI antibody titre is below log2 4 after vaccination and only 20-30% were survived. Based on the post-mortem findings on the survived chicken from each vaccinated group, their internal organs appeared normal and no sign of haemorrhage or pathognomonic signs of Newcastle disease (ND). Conclusively, vaccinated chicken are effectively protected from morbidity and mortality against virulent genotype VII challenge with the addition of adjuvant into inactivated local strain of NDV genotype VII vaccine. Thus, the development of inactivated local NDV genotype VII vaccine is a promising candidate to control the current ND endemic in Malaysia. 

scholarly journals Protective efficacy of inactivated Newcastle disease virus vaccines prepared in two different oil-based adjuvants

2020 ◽  
Vol 87 (1) ◽  
Author(s):  
Oday A. Aljumaili ◽  
Muhammad B. Bello ◽  
Swee K. Yeap ◽  
Abdul R. Omar ◽  
Aini Ideris
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Protective Efficacy ◽  
Clinical Disease ◽  
Virus Shedding ◽  
Live Attenuated Vaccines ◽  
Inactivated Vaccines ◽  
Highly Virulent ◽  
Genotype Vii

Despite the availability of Newcastle disease (ND) vaccines for more than six decades, disease outbreaks continue to occur with huge economic consequences to the global poultry industry. The aim of this study is to develop a safe and effective inactivated vaccine based on a recently isolated Newcastle disease virus (NDV) strain IBS025/13 and evaluate its protective efficacy in chicken following challenge with a highly virulent genotype VII isolate. Firstly, high titre of IBS025/13 was exposed to various concentrations of binary ethylenimine (BEI) to determine the optimal conditions for complete inactivation of the virus. The inactivated virus was then prepared in form of a stable water-in-oil emulsion of black seed oil (BSO) or Freund’s incomplete adjuvant (FIA) and used as vaccines in specific pathogen-free chicken. Efficacy of various vaccine preparations was also evaluated based on the ability of the vaccine to protect against clinical disease, mortality and virus shedding following challenge with highly virulent genotype\VII NDV isolate. The results indicate that exposure of NDV IBS025/13 to 10 mM of BEI for 21 h at 37 °C could completely inactivate the virus without tempering with the structural integrity of the viral hemagglutin-neuraminidase protein. More so, the inactivated vaccines adjuvanted with either BSO- or FIA-induced high hemagglutination inhibition antibody titre that protected the vaccinated birds against clinical disease and in some cases virus shedding, especially when used together with live attenuated vaccines. Thus, genotype VII-based NDV-inactivated vaccines formulated in BSO could substantially improve poultry disease control particularly when combined with live attenuated vaccines.

scholarly journals Single-Dose Vaccination of Recombinant Chimeric Newcastle Disease Virus (NDV) LaSota Vaccine Strain Expressing Infectious Bursal Disease Virus (IBDV) VP2 Gene Provides Full Protection against Genotype VII NDV and IBDV Challenge

Vaccines ◽  
2021 ◽  
Vol 9 (12) ◽  
pp. 1483
Author(s):  
Qilong Qiao ◽  
Mingzhen Song ◽  
Congcong Song ◽  
Yihang Zhang ◽  
Xiangdong Wang ◽  
...  
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Vaccine Strain ◽  
Newcastle Disease ◽  
Infectious Bursal Disease Virus ◽  
Infectious Bursal Disease ◽  
Vp2 Gene ◽  
Bursal Disease ◽  
Full Protection ◽  
Genotype Vii

Newcastle disease virus (NDV) and infectious bursal disease virus (IBDV) are the two most important and widespread viruses causing huge economic losses in the global poultry industry. Outbreaks of genotype VII NDV and IBDV variants in vaccinated poultry flocks call for genetically matched vaccines. In the present study, a genetic matched chimeric NDV LaSota vaccine strain expressing VP2 gene of IBDV variant, rLaS-VIIF/HN-VP2 was generated for the first time, in which both the F and HN genes of LaSota were replaced with those of the genotype VII NDV strain FJSW. The cleavage site of the FJSW strain F protein in the rLaS-VIIF/HN-VP2 genome was mutated to the avirulent motif found in LaSota. Expression of IBDV VP2 protein was confirmed by western blot. The rLaS-VIIF/HN-VP2 maintained the efficient replication ability in embryonated eggs, low pathogenicity and genetic stability comparable to that of parental LaSota virus. One dose oculonasal vaccination of one-week-old SPF chickens with rLaS-VIIF/HN-VP2 induced full protection against genotype VII NDV and IBDV lethal challenge. These results indicate that the rLaS-VIIF/HN-VP2 is a promising bivalent vaccine to prevent infections of IBDV and genotype VII NDV.

scholarly journals Genome Sequences of Newly Emerged Newcastle Disease Virus Strains Isolated from Disease Outbreaks in Indonesia

2020 ◽  
Vol 9 (23) ◽  
Author(s):  
Mohammad Rabiei ◽  
Mohamad Indro Cahyono ◽  
Phuong Thi Kim Doan ◽  
Putri Pandarangga ◽  
Simson Tarigan ◽  
...  
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Disease Outbreaks ◽  
Phylogenetic Study ◽  
Genome Sequences ◽  
Gene Coding ◽  
Study Results ◽  
Virus Strains ◽  
Genotype Vii

ABSTRACT Here, we report two genomes of newly emerged strains of Newcastle disease virus (NDV), Chicken/Indonesia/Tangerang/004WJ/14 and Chicken/Indonesia/VD/003WJ/11, from disease outbreaks in chickens in Indonesia. Phylogenetic study results of the fusion (F) protein’s gene-coding sequences of different genotypes of NDV revealed that these two strains belong to genotype VII.2 in the class II cluster of avian paramyxoviruses.

scholarly journals Requirements for the Assembly and Release of Newcastle Disease Virus-Like Particles

2006 ◽  
Vol 80 (22) ◽  
pp. 11062-11073 ◽  
Author(s):  
Homer D. Pantua ◽  
Lori W. McGinnes ◽  
Mark E. Peeples ◽  
Trudy G. Morrison
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Plasma Membranes ◽  
Matrix Protein ◽  
Viral Proteins ◽  
M Protein ◽  
Virion Assembly ◽  
Virus Like Particles ◽  
Necessary And Sufficient

ABSTRACT Paramyxoviruses, such as Newcastle disease virus (NDV), assemble in and bud from plasma membranes of infected cells. To explore the role of each of the NDV structural proteins in virion assembly and release, virus-like particles (VLPs) released from avian cells expressing all possible combinations of the nucleoprotein (NP), membrane or matrix protein (M), an uncleaved fusion protein (F-K115Q), and hemagglutinin-neuraminidase (HN) protein were characterized for densities, protein content, and efficiencies of release. Coexpression of all four proteins resulted in the release of VLPs with densities and efficiencies of release (1.18 to 1.16 g/cm3 and 83.8% ± 1.1%, respectively) similar to those of authentic virions. Expression of M protein alone, but not NP, F-K115Q, or HN protein individually, resulted in efficient VLP release, and expression of all different combinations of proteins in the absence of M protein did not result in particle release. Expression of any combination of proteins that included M protein yielded VLPs, although with different densities and efficiencies of release. To address the roles of NP, F, and HN proteins in VLP assembly, the interactions of proteins in VLPs formed with different combinations of viral proteins were characterized by coimmunoprecipitation. The colocalization of M protein with cell surface F and HN proteins in cells expressing all combinations of viral proteins was characterized. Taken together, the results show that M protein is necessary and sufficient for NDV budding. Furthermore, they suggest that M-HN and M-NP interactions are responsible for incorporation of HN and NP proteins into VLPs and that F protein is incorporated indirectly due to interactions with NP and HN protein.

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