Immunohistochemical investigation of the cross-reactivity of selected cell markers in formalin-fixed, paraffin-embedded lymphoid tissues of Franciscana ( Pontoporia blainvillei )

2018 ◽  
Vol 200 ◽  
pp. 52-58 ◽  
Author(s):  
J. Díaz-Delgado ◽  
R. Ressio ◽  
K.R. Groch ◽  
J.L. Catão-Dias
Keyword(s):  
Cross Reactivity ◽  
Lymphoid Tissues ◽  
Cell Markers ◽  
Immunohistochemical Investigation ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
The Cross ◽  
Pontoporia Blainvillei ◽  
Formalin Fixed

scholarly journals Standardization and Validation of an Immunoperoxidase Assay for the Detection of African Horse Sickness Virus in Formalin-Fixed, Paraffin-Embedded Tissues

2009 ◽  
Vol 21 (5) ◽  
pp. 655-667 ◽  
Author(s):  
Sarah J. Clift ◽  
Mark C. Williams ◽  
Truuske Gerdes ◽  
Marie M. E. Smit
Keyword(s):  
Detection System ◽  
Cross Reactivity ◽  
African Horse Sickness ◽  
African Horse Sickness Virus ◽  
Standard Procedures ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Complex Detection ◽  
Immunoperoxidase Assay ◽  
Formalin Fixed

An immunoperoxidase assay for the detection of African horse sickness virus (AHSV) in formalin-fixed tissues is a valuable tool in the study of the pathogenesis of the disease, as well as a useful addition to existing diagnostic tests when only preserved tissues are available. An assay that uses Hamblin antiserum in a basic avidin-biotin complex detection system was standardized and validated in accordance with the guidelines of the American Association of Veterinary Laboratory Diagnosticians Subcommittee on Standardization of Immunohistochemistry. Using 128 positive cases of African horse sickness confirmed by viral isolation and serotyping and 119 negative cases from countries where the disease has never occurred, diagnostic sensitivity and diagnostic specificity were 100% in the prime target tissues of heart and lung. There was no variation in the ability of the assay to detect all 9 serotypes of AHSV, and there was no cross-reactivity with other orbiviruses in formalin-fixed tissues. The only cross-reactivity observed was in the lungs of 2 negative cases infected with Rhodococcus equi. The assay gave good results on tissues that had been fixed in formalin for up to 365 days. Nonspecific staining was minimal provided that the standard procedures for processing and staining tissues were followed. Good immunohistochemical results were also obtained on samples fixed as long as 24 hr after death. The assay, therefore, provides a robust diagnostic tool for detection of AHSV in formalin-fixed tissues, provided the analysis is done by an experienced pathologist.

scholarly journals Immunomycology: rapid and specific immunocytochemical identification of fungi in formalin-fixed, paraffin-embedded material.

1993 ◽  
Vol 41 (8) ◽  
pp. 1217-1221 ◽  
Author(s):  
J A Reed ◽  
B A Hemann ◽  
J L Alexander ◽  
D J Brigati
Keyword(s):  
Complement Fixation ◽  
Fungal Infections ◽  
Periodic Acid ◽  
Cross Reactivity ◽  
Periodic Acid Schiff ◽  
Formalin Fixed Paraffin ◽  
Identification Of Fungi ◽  
Formalin Fixed Paraffin Embedded ◽  
Methenamine Silver ◽  
Formalin Fixed

We report the rapid (less than 1 hr), immunocytochemical identification of various fungi in formalin-fixed, paraffin-embedded tissues using antisera originally developed for use in immunodiffusion assays. Primary antisera directed towards fungal genera including Aspergillus, Blastomyces, Candida, Coccidioides, Cryptococcus, Histoplasma, and Sporothrix were examined. The specificity of each antiserum was evaluated by the presence or absence of crossreactivity with other morphologically similar fungi in both paraffin-embedded pure fungal cultures and tissues with culture-confirmed fungal infections. Each antiserum reacted strongly with the fungus to which it had been raised, whether examined in pure culture or infected tissues. The antisera raised against Candida, Cryptococcus, and Sporothrix did not exhibit cross-reactivity with any other fungus tested. However, the antisera raised to Aspergillus, Blastomyces, Coccidioides, and Histoplasma demonstrated significant crossreactivity with other genera of fungi, thus precluding their routine use in diagnostic immunocytochemistry. The results indicate that immunocytochemistry may provide an important adjunct to other methods, such as immunodiffusion or complement fixation assays and histochemical stains such as the Grocott methenamine silver or periodic acid-Schiff, when attempts are made to specifically identify certain fungi in formalin-fixed, paraffin-embedded tissues before mycology culture results are available.

scholarly journals Immunohistochemical Detection of Haemophilus Parasuis Serovar 5 in Formalin-Fixed, Paraffin-Embedded Tissues of Experimentally Infected Swine

1997 ◽  
Vol 9 (3) ◽  
pp. 237-243 ◽  
Author(s):  
Joaquim Segalés ◽  
Mariano Domingo ◽  
Gloria I. Solano ◽  
Carlos Pijoan
Keyword(s):  
Inflammatory Cell ◽  
Cross Reactivity ◽  
Polyclonal Antiserum ◽  
Gram Negative Bacteria ◽  
Haemophilus Parasuis ◽  
Retrospective Diagnosis ◽  
Formalin Fixed Paraffin ◽  
Rabbit Polyclonal Antiserum ◽  
Formalin Fixed Paraffin Embedded ◽  
Formalin Fixed

An avidin-biotin complex immunohistochemistry technique was developed to detect Haemophilus parasuis serovar 5 in experimentally infected 18-21-day-old conventional pigs, using a rabbit polyclonal antiserum. Seven of 10 intratracheally inoculated animals developed a low to medium degree of fibrinous polyserositis; meninges and pleura were the most severely affected areas. Haemophilus parasuis was recovered from 9 of 10 pigs; in 2 of them H. parasuis was isolated from tracheal swabs only. Positive immunohistochemistry results, mainly observed as free bacteria or bacteria within inflammatory cell cytoplasm in the fibrinopurulent exudate, were observed in 8 of 10 animals. Cross-reactivity with Actinobacillus pleuropneumoniae was detected but not with other gram-positive and gram-negative bacteria tested. This immunohistochemistry technique seemed to be at least as sensitive as microbiologic cultures and could be useful in studies of pathogenesis and retrospective diagnosis. However, cross-reactivity with A. pleuropneumoniae means that positive immunohistochemistry results in lung tissue from field cases would be dubious.

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