Intestinal colonization and acute immune response in commercial turkeys following inoculation with Campylobacter jejuni constructs encoding antibiotic-resistance markers

The present study was conducted to investigate the prevalence and antibiotic resistance among Campylobacter jejuni in ulam at farms and retail outlets located in Kuala Terengganu, Malaysia. A total of 526 samples (ulam, soil, and fertilizer) were investigated for the presence of C. jejuni and the gene for cytolethal distending toxin (cdt) by using a multiplex PCR method. Antibiotic susceptibility to 10 types of antibiotics was determined using the disk diffusion method for 33 C. jejuni isolates. The average prevalence of contaminated samples from farms, wet markets, and supermarkets was 35.29, 52.66, and 69.88%, respectively. The cdt gene was not detected in 24 of the 33 C. jejuni isolates, but 9 isolates harbored cdtC. Antibiotic resistance in C. jejuni isolates was highest to penicillin G (96.97% of isolates) followed by vancomycin (87.88%), ampicillin (75.76%), erythromycin (60.61%), tetracycline (9.09%), amikacin (6.06%), and norfloxacin (3.03%); none of the isolates were resistant to ciprofloxacin, enrofloxacin, and gentamicin. In this study, C. jejuni was present in ulam, and some isolates were highly resistant to some antibiotics but not to quinolones. Thus, appropriate attention and measures are required to prevent C. jejuni contamination on farms and at retail outlets.

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Presence of Lipooligosaccharide Biosynthesis Genes Associated with Ganglioside Mimicry as a Cause of Guillain^|^ndash;Barr^|^eacute; Syndrome and Antibiotic Resistance of Campylobacter jejuni Strains from Gastroenteritis Patients and Chicken

Japanese Journal of Food Microbiology ◽

10.5803/jsfm.30.39 ◽

2013 ◽

Vol 30 (1) ◽

pp. 39-42

Author(s):

Masanori MATSUDA ◽

Tomoko TOKUSHIMA ◽

Kumiko SHIGEMURA ◽

Hiroshi HIWAKI ◽

Munenori FURUTA ◽

...

Keyword(s):

Antibiotic Resistance ◽

Campylobacter Jejuni

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Immune response to Campylobacter jejuni and Campylobacter coli in a cohort of children from birth to 2 years of age.

Infection and Immunity ◽

10.1128/iai.57.8.2542-2546.1989 ◽

1989 ◽

Vol 57 (8) ◽

pp. 2542-2546 ◽

Cited By ~ 34

Author(s):

P M Martin ◽

J Mathiot ◽

J Ipero ◽

M Kirimat ◽

A J Georges ◽

...

Keyword(s):

Immune Response ◽

Campylobacter Jejuni ◽

Campylobacter Coli

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Postchill Campylobacter Prevalence on Broiler Carcasses in Relation to Slaughter Group Colonization Level and Chilling System

Journal of Food Protection ◽

10.4315/0362-028x-69.3.495 ◽

2006 ◽

Vol 69 (3) ◽

pp. 495-499 ◽

Cited By ~ 17

Author(s):

M. LINDBLAD ◽

I. HANSSON ◽

I. VÅGSHOLM ◽

R. LINDQVIST

Keyword(s):

Campylobacter Jejuni ◽

Surveillance Program ◽

Intestinal Colonization ◽

Direct Plating ◽

Baseline Study ◽

Group Data ◽

Low Degree ◽

High Degree ◽

Campylobacter Spp ◽

Skin Samples

Data from an ongoing national surveillance program of Campylobacter prevalence in broiler slaughter groups were related to results from a 1-year baseline study of broiler carcasses postchill. The goals were to establish the relation between Campylobacter prevalence in slaughter groups and on carcasses and to determine the effect of various chilling systems on Campylobacter prevalence. Pooled cloacal and neck skin samples from the surveillance program were analyzed after enrichment. Carcass rinse samples from the baseline study were analyzed after enrichment and by direct plating. Data from both studies were available for 614 carcasses. Direct-plating analyses indicated that the percentages of carcasses positive for Campylobacter jejuni and other Campylobacter spp. in slaughter groups with negative cloacal samples were 2 and 10%, respectively, whereas enrichment analyses indicated prevalences of 2% in both cases. Campylobacter prevalence in slaughter groups with a high degree of intestinal colonization (more than half of the pooled cloacal samples positive) was significantly higher than in slaughter groups with a low degree of colonization (76 to 85% and 30 to 50%, respectively, depending on Campylobacter spp. and analytical method). The prevalence of Campylobacter-positive carcasses postchill was at the same level as the prevalence of carcasses that originated from slaughter groups with positive neck skin samples at four of the six slaughterhouses. Only at one slaughterhouse, with an air-chilling system, was the postchill prevalence (13%) lower than that expected from slaughter group data (23%). The postchill prevalence (43%) was higher than that expected from slaughter group data (33%) at one slaughterhouse with immersion chilling.

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