Multiple locus variable number tandem repeat analysis for the characterization of wild feline Bartonella species and subspecies

2020 ◽  
Vol 247 ◽  
pp. 108788
Author(s):  
Henri-Jean Boulouis ◽  
Bruno B. Chomel ◽  
Girault Guillaume ◽  
Durand Benoît ◽  
Chao-chin Chang ◽  
...  
2015 ◽  
Vol 64 (3) ◽  
pp. 288-294 ◽  
Author(s):  
Nobuo Koizumi ◽  
Maki Mizutani Muto ◽  
Hidemasa Izumiya ◽  
Motoi Suzuki ◽  
Makoto Ohnishi

2009 ◽  
Vol 136 (3-4) ◽  
pp. 277-284 ◽  
Author(s):  
Risma Ikawaty ◽  
E.C. Brouwer ◽  
M.D. Jansen ◽  
E. van Duijkeren ◽  
D. Mevius ◽  
...  

Author(s):  
Urška Glinšek Biškup ◽  
Rok Kogoj ◽  
Miša Korva ◽  
Nataša Knap ◽  
Tjaša Cerar ◽  
...  

2004 ◽  
Vol 186 (16) ◽  
pp. 5496-5505 ◽  
Author(s):  
Leo M. Schouls ◽  
Han G. J. van der Heide ◽  
Luc Vauterin ◽  
Paul Vauterin ◽  
Frits R. Mooi

ABSTRACT Bordetella pertussis, the causative agent of whooping cough, has remained endemic in The Netherlands despite extensive nationwide vaccination since 1953. In the 1990s, several epidemic periods have resulted in many cases of pertussis. We have proposed that strain variation has played a major role in the upsurges of this disease in The Netherlands. Therefore, molecular characterization of strains is important in identifying the causes of pertussis epidemiology. For this reason, we have developed a multiple-locus variable-number tandem repeat analysis (MLVA) typing system for B. pertussis. By combining the MLVA profile with the allelic profile based on multiple-antigen sequence typing, we were able to further differentiate strains. The relationships between the various genotypes were visualized by constructing a minimum spanning tree. MLVA of Dutch strains of B. pertussis revealed that the genotypes of the strains isolated in the prevaccination period were diverse and clearly distinct from the strains isolated in the 1990s. Furthermore, there was a decrease in diversity in the strains from the late 1990s, with a remarkable clonal expansion that coincided with the epidemic periods. Using this genotyping, we have been able to show that B. pertussis is much more dynamic than expected.


2020 ◽  
Vol 249 ◽  
pp. 108836
Author(s):  
Dorottya Földi ◽  
Katinka Bekő ◽  
Orsolya Felde ◽  
Zsuzsa Kreizinger ◽  
Áron B. Kovács ◽  
...  

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