scholarly journals Mutations in the carboxyl terminal region of E2 glycoprotein of classical swine fever virus are responsible for viral attenuation in swine

Virology ◽  
2007 ◽  
Vol 364 (2) ◽  
pp. 371-382 ◽  
Author(s):  
G.R. Risatti ◽  
L.G. Holinka ◽  
I. Fernandez Sainz ◽  
C. Carrillo ◽  
G.F. Kutish ◽  
...  
Keyword(s):  
Classical Swine Fever Virus ◽  
Classical Swine Fever ◽  
Terminal Region ◽  
Fever Virus ◽  
E2 Glycoprotein ◽  
Carboxyl Terminal ◽  
Viral Attenuation

Identification of conformational epitopes and antigen-specific residues at the D/A domains and the extramembrane C-terminal region of E2 glycoprotein of classical swine fever virus

2012 ◽  
Vol 168 (1-2) ◽  
pp. 56-63 ◽  
Author(s):  
Chia-Yi Chang ◽  
Chin-Cheng Huang ◽  
Ming-Chung Deng ◽  
Yeou-Liang Huang ◽  
Yu-Ju Lin ◽  
...  
Keyword(s):  
Classical Swine Fever Virus ◽  
Classical Swine Fever ◽  
Terminal Region ◽  
Fever Virus ◽  
E2 Glycoprotein ◽  
Conformational Epitopes ◽  
A Domains

scholarly journals The Unique Glycosylation at Position 986 on the E2 Glycoprotein of Classical Swine Fever Virus Is Responsible for Viral Attenuation and Protection against Lethal Challenge

2021 ◽  
Author(s):  
Yongfeng Li ◽  
Mengqi Yuan ◽  
Yuying Han ◽  
Libao Xie ◽  
Yuteng Ma ◽  
...  
Keyword(s):  
Viral Replication ◽  
Classical Swine Fever Virus ◽  
Virulent Strain ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Lethal Challenge ◽  
E2 Glycoprotein ◽  
Viral Virulence ◽  
Viral Attenuation ◽  
Highly Virulent

Classical swine fever (CSF) is an economically important disease of pigs caused by the classical swine fever virus (CSFV). The live attenuated vaccine C-strain (also called HCLV strain) against CSF was produced by multiple passages of a highly virulent strain in rabbits. However, the molecular determinants for its attenuation and protection remain unclear. In this study, we identified a unique glycosylation at position 986 ( 986 NYT 988 ) on the E2 glycoprotein Domain IV of C-strain but not ( 986 NYA 988 ) the highly virulent CSFV Shimen strain. We evaluated the infectivity, virulence, and protective efficacy of the C-strain-based mutant rHCLV-T988A lacking the glycosylation and Shimen strain mutant rShimen-A988T acquiring an additional glycosylation at position 986. rShimen-A988T showed a significantly decreased viral replication ability in SK6 cells, while rHCLV-T988A exhibited a growth kinetics indistinguishable from that of C-strain. Removal of the C-strain glycosylation site does not affect viral replication in rabbits and the attenuated phenotype in pigs. However, rShimen-A988T was attenuated and protected the pigs from a lethal challenge at 14 days post-inoculation. In contrast, the rHCLV-T988A-inoculated pigs showed transient fever, a few clinical signs, and pathological changes in the spleens upon challenge with the Shimen strain. Mechanistic investigations revealed that the unique glycosylation at position 986 influences viral spreading, alters the formation of E2 homodimers, and leads to increased production of neutralizing antibodies. Collectively, our data for the first time demonstrate that the unique glycosylation at position 986 on the E2 glycoprotein is responsible for viral attenuation and protection. IMPORTANCE Viral glycoproteins involve in infectivity, virulence, and host immune responses. Deglycosylation on the E rns , E1, or E2 glycoprotein of highly virulent classical swine fever virus (CSFV) attenuated viral virulence in pigs, indicating that the glycosylation contributes to the pathogenicity of highly virulent strain. However, the effects of the glycosylation on the C-strain E2 glycoprotein on viral infectivity in cells, viral attenuation, and protection in pigs have not been elucidated. This study demonstrates the unique glycosylation at position 986 on the C-strain E2 glycoprotein. C-strain mutant removing the glycosylation at the site provides only partial protection against CSFV challenge. Remarkably, the addition of the glycan to E2 of the highly virulent Shimen strain attenuates the viral virulence and confers complete protection against the lethal challenge in pigs. Our findings provide a new insight into the contribution of the glycosylation to the virus attenuation and protection.

scholarly journals A Novel E2 Glycoprotein Subunit Marker Vaccine Produced in Plant Is Able to Prevent Classical Swine Fever Virus Vertical Transmission after Double Vaccination

Vaccines ◽  
2021 ◽  
Vol 9 (5) ◽  
pp. 418
Author(s):  
Youngmin Park ◽  
Yeonsu Oh ◽  
Miaomiao Wang ◽  
Llilianne Ganges ◽  
José Alejandro Bohórquez ◽  
...  
Keyword(s):  
Vertical Transmission ◽  
Classical Swine Fever Virus ◽  
Neutralizing Antibodies ◽  
Subunit Vaccine ◽  
Vaccine Dose ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Tissue Samples ◽  
Marker Vaccine ◽  
E2 Glycoprotein

The efficacy of a novel subunit vaccine candidate, based in the CSFV E2 glycoprotein produced in plants to prevent classical swine fever virus (CSFV) vertical transmission, was evaluated. A Nicotiana benthamiana tissue culture system was used to obtain a stable production of the E2-glycoprotein fused to the porcine Fc region of IgG. Ten pregnant sows were divided into three groups: Groups 1 and 2 (four sows each) were vaccinated with either 100 μg/dose or 300 μg/dose of the subunit vaccine at 64 days of pregnancy. Group 3 (two sows) was injected with PBS. Groups 1 and 2 were boosted with the same vaccine dose. At 10 days post second vaccination, the sows in Groups 2 and 3 were challenged with a highly virulent CSFV strain. The vaccinated sows remained clinically healthy and seroconverted rapidly, showing efficient neutralizing antibodies. The fetuses from vaccinated sows did not show gross lesions, and all analyzed tissue samples tested negative for CSFV replication. However, fetuses of non-vaccinated sows had high CSFV replication in tested tissue samples. The results suggested that in vaccinated sows, the plant produced E2 marker vaccine induced the protective immunogenicity at challenge, leading to protection from vertical transmission to fetuses.

scholarly journals Research Article An improved Bac-to-Bac/BmNPV technology expressing envelope E2 glycoprotein of classical swine fever virus (CSFV) in the silkworm, Bombyx mori

2019 ◽  
Vol 18 (1) ◽  
Author(s):  
X. Hu ◽  
Y. Chen ◽  
C. O. Singh ◽  
S. Zhang ◽  
Y.G. Miao ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Classical Swine Fever Virus ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Research Article ◽  
E2 Glycoprotein

Molecular characterization of E2 glycoprotein of classical swine fever virus: adaptation and propagation in porcine kidney cells

2015 ◽  
Vol 51 (5) ◽  
pp. 441-446 ◽  
Author(s):  
Rakesh Kumar ◽  
Nagendra N. Barman ◽  
Elina Khatoon ◽  
Gitika Rajbongshi ◽  
Nipu Deka ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Classical Swine Fever Virus ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Kidney Cells ◽  
Porcine Kidney ◽  
E2 Glycoprotein ◽  
Virus Adaptation

Sequence Analysis of E2 Glycoprotein from Indian Isolate of Classical Swine Fever Virus (CSFV)

2015 ◽  
Vol 43 (1) ◽  
pp. 22-30
Author(s):  
Mehak Bajwa ◽  
Ramneek Verma ◽  
Dipak Deka ◽  
Gagandeep Singh Dhol ◽  
Nagendra Nath Barman
Keyword(s):  
Sequence Analysis ◽  
Classical Swine Fever Virus ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Indian Isolate ◽  
E2 Glycoprotein

scholarly journals The E2 glycoprotein is necessary but not sufficient for the adaptation of classical swine fever virus lapinized vaccine C-strain to the rabbit

Virology ◽  
2018 ◽  
Vol 519 ◽  
pp. 197-206 ◽  
Author(s):  
Yongfeng Li ◽  
Libao Xie ◽  
Lingkai Zhang ◽  
Xiao Wang ◽  
Chao Li ◽  
...  
Keyword(s):  
Classical Swine Fever Virus ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
E2 Glycoprotein

N-Glycosylation Pattern of E2 Glycoprotein from Classical Swine Fever Virus

2009 ◽  
Vol 8 (2) ◽  
pp. 546-555 ◽  
Author(s):  
Raquel Montesino ◽  
Jorge R. Toledo ◽  
Oliberto Sánchez ◽  
Yasser Zamora ◽  
Maritza Barrera ◽  
...  
Keyword(s):  
Classical Swine Fever Virus ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Glycosylation Pattern ◽  
E2 Glycoprotein

Synergistic roles of the E2 glycoprotein and 3′ untranslated region in the increased genomic stability of chimeric classical swine fever virus with attenuated phenotypes

2017 ◽  
Vol 162 (9) ◽  
pp. 2667-2678 ◽  
Author(s):  
Rui Wu ◽  
Ling Li ◽  
Lei Lei ◽  
Cheng Zhao ◽  
Xiaofang Shen ◽  
...  
Keyword(s):  
Classical Swine Fever Virus ◽  
Untranslated Region ◽  
Genomic Stability ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
E2 Glycoprotein

The N-glycosylation of classical swine fever virus E2 glycoprotein extracellular domain expressed in the milk of goat

2010 ◽  
Vol 500 (2) ◽  
pp. 169-180 ◽  
Author(s):  
Raquel Montesino ◽  
Jeovanis Gil ◽  
Luis J. González ◽  
Yasser Zamora ◽  
Louise Royle ◽  
...  
Keyword(s):  
Classical Swine Fever Virus ◽  
Classical Swine Fever ◽  
Extracellular Domain ◽  
Fever Virus ◽  
E2 Glycoprotein
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