Horizontal pollen transmission of Gentian ovary ring-spot virus is initiated during penetration of the stigma and style by infected pollen tubes

Montmorency sour cherry trees, 3 years of age and older, became infected more often with necrotic ring spot virus when exposed during May than when caged individually under 32-mesh screen during May. In screened compartments, necrotic ring spot spread only when bees were present during blossoming. Though necrotic ring spot spread to all but 1 untreated tree in a bearing orchard, it did not spread to neighbouring trees from which blossom buds were removed. Finally, necrotic ring spot virus was transferred to at least 4, and sour cherry yellows virus to at least 1 and possibly a second, of 14 trees that were emasculated and then pollinated with pollen from diseased trees. It is concluded that the viruses causing these diseases are transmitted from one cherry tree to another through pollination.

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Comparison of ELISA and RT-PCR for the detection of Prunus necrotic ring spot virus and prune dwarf virus in almond (Prunus dulcis)

Journal of Virological Methods ◽

10.1016/j.jviromet.2003.08.014 ◽

2003 ◽

Vol 114 (1) ◽

pp. 65-69 ◽

Cited By ~ 24

Author(s):

Genet Mekuria ◽

Sunita A. Ramesh ◽

Evita Alberts ◽

Terry Bertozzi ◽

Michelle Wirthensohn ◽

...

Keyword(s):

Prunus Dulcis ◽

Dwarf Virus ◽

Rt Pcr ◽

Spot Virus ◽

Prune Dwarf Virus ◽

Ring Spot

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Properties of the single protein and two nucleic acids of tomato ring-spot virus

Canadian Journal of Botany ◽

10.1139/b77-120 ◽

1977 ◽

Vol 55 (8) ◽

pp. 1028-1037 ◽

Cited By ~ 8

Author(s):

Wayne R. Allen ◽

H. F. Dias

Keyword(s):

Nucleic Acid ◽

Nucleic Acids ◽

Gel Electrophoresis ◽

Average Molecular Weight ◽

Virus Genome ◽

Protein Subunit ◽

Spot Virus ◽

Single Protein ◽

Middle Component ◽

Ring Spot

Purified preparations of several isolates of tomato ring-spot virus were shown by rate-zonal centrifugation in sucrose and equilibrium centrifugation in CsCl to be composed of two individual nucleoprotein components. Acrylamide-gel electrophoresis showed that the lighter (middle) component contained a nucleic acid (RNA 2) that was distinct from the species (RNA 1) contained in the heavier (bottom) component. The bottom was more infectious than the middle component and infectivity was enhanced by mixing the components, indicating that the virus genome is divided between component types. Similar results were obtained from infectivity tests on the two nucleic acids. The nucleic acid contents of the middle and bottom components were about 40 and 41%, respectively. The average molecular weights of RNA 2 and RNA 1 from three virus isolates, as determined by acrylamide-gel electrophoresis, were 2.5 and 2.6 × 106, respectively. Molecular complexing between the RNA species during electrophoresis was prevented with the use of formamide. The single protein subunit from the same three isolates had an average molecular weight of about 58 000. Serological comparisons of five tomato ring-spot isolates associated with diseases of fruit trees and grapevines indicated that only the grape yellow vein strain was antigenically distinct. These and other properties indicate that this virus is similar to other members of the nepovirus group.

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