Expression of a neurotoxin gene improves the insecticidal activity of Spodoptera litura nucleopolyhedrovirus (SpltNPV)

2011 ◽  
Vol 159 (1) ◽  
pp. 51-56 ◽  
Author(s):  
Xin-Xin Tang ◽  
Xing-Lu Sun ◽  
Guan-Qin Pu ◽  
Wen-Bing Wang ◽  
Chuan-Xi Zhang ◽  
...  
2005 ◽  
Vol 5 (1) ◽  
pp. 11-16
Author(s):  
Hamdani Hamdani

Activity of Melia azedarach (L.) seed extract against armyworm Spodoptera litura (F.) (Lepidoptera: Noctuidae). This study was conducted to evaluate the effectivenes and biological activity of Melia azedarach L. (Meliaceae) seed extract against armyworm, Spodoptera litura F. The first instar larvae were fed extract-treated cotton leaves for 2 days, then were maintained on untreated leaves until the third instar stage. Records were kept in regard to the larvae mortality and developmental time of surviving larvae from first instar to third instar. The result showed that Melia azedarach L. seed extract at consentration of 50 g of seeds/l of water (5%) exhibited moderate insecticidal activity against S. litura larvae (43.33 - 68.33% mortality). Addition of detergen at 0.2% to extract did not increase insecticidal activity of the extract. However, boiling seed extract at consentration of 50 g of seeds/l of water (5%) during 10 until 20 minutes increased insecticidal activity of extract (66.67 - 68.33% mortality). Generally, M. azedarach seed extract treatment did not affect  developmental time of  S. litura larvae.


2016 ◽  
Vol 26 (10) ◽  
pp. 1774-1780 ◽  
Author(s):  
Feifei Song ◽  
Yunfeng Lin ◽  
Chen Chen ◽  
Ensi Shao ◽  
Xiong Guan ◽  
...  

2019 ◽  
Vol 76 (3) ◽  
pp. 928-935 ◽  
Author(s):  
Sorachat Tharamak ◽  
Thitaree Yooboon ◽  
Anchulee Pengsook ◽  
Atcharee Ratwatthananon ◽  
Nutchaya Kumrungsee ◽  
...  

Toxins ◽  
2020 ◽  
Vol 12 (4) ◽  
pp. 274 ◽  
Author(s):  
Ensi Shao ◽  
Aishan Zhang ◽  
Yaqi Yan ◽  
Yaomin Wang ◽  
Xinyi Jia ◽  
...  

Bacillus thuringiensis (Bt) Vip3A proteins are important insecticidal proteins used for control of lepidopteran insects. However, the mode of action of Vip3A toxin is still unclear. In this study, the amino acid residue S164 in Vip3Aa was identified to be critical for the toxicity in Spodoptera litura. Results from substitution mutations of the S164 indicate that the insecticidal activity of Vip3Aa correlated with the formation of a >240 kDa complex of the toxin upon proteolytic activation. The >240 kDa complex was found to be composed of the 19 kDa and the 65 kDa fragments of Vip3Aa. Substitution of the S164 in Vip3Aa protein with Ala or Pro resulted in loss of the >240 kDa complex and loss of toxicity in Spodoptera litura. In contrast, substitution of S164 with Thr did not affect the >240 kDa complex formation, and the toxicity of the mutant was only reduced by 35%. Therefore, the results from this study indicated that formation of the >240 kDa complex correlates with the toxicity of Vip3Aa in insects and the residue S164 is important for the formation of the complex.


2013 ◽  
Vol 70 (3) ◽  
pp. 510-516 ◽  
Author(s):  
Si-Han Huang ◽  
Ji-Dong Xian ◽  
Song-Zhi Kong ◽  
Yu-Cui Li ◽  
Jian-Hui Xie ◽  
...  

2021 ◽  
Vol 306 ◽  
pp. 01055
Author(s):  
Ade Aliyya ◽  
Apriwi Zulfitri ◽  
Umi Zakiah ◽  
Arief Heru Prianto ◽  
Titik Kartika ◽  
...  

Chemical pesticide has been used extensively to control Spodoptera litura that attack economically important crops.The use of synthetic pesticides with high intensity is detrimental to the quality of life. The objective of this study was to evaluate the insecticidal activity of entomopathogenic fungi and neem-based biopesticide on S. litura using spraying and forced feeding method. The treatments were spore solution of Trichoderma viride, Metarhizium sp. T4.B23, Metarhizium sp. B2.2 applied at 108 spores/mL, biopesticide formulation containing neem extract (Agr1) at 10% (v/v), and water as control. Each treatment was sprayed 0.3 mL directly onto larvae of S. litura while in forced feeding method, the larvae were fed on 1 gr of treated water spinach. Entomopathogenic fungal spore solution did not show efficacy on S litura larvae in both methods, while Agr 1 caused 90 and 40% mortality in spraying and forced feeding method, respectively. Furthermore, the efficacy of Agr 1 was compared to Agr 2 that contains neem extract and wood vinegar. Agr 2 caused 100% mortality when sprayed onto S. litura larvae, and more than 90% of larvae were died when forced to feed treated water spinach. Agr2 has better potential than Agr1 to control S. litura.


2020 ◽  
Vol 76 (6) ◽  
pp. 2004-2014
Author(s):  
Prakash Y Shankhu ◽  
Chetna Mathur ◽  
Abhishek Mandal ◽  
Doddachowdappa Sagar ◽  
Vishal S Somvanshi ◽  
...  

2004 ◽  
Vol 29 (4) ◽  
pp. 372-375 ◽  
Author(s):  
Shigeru Saito ◽  
Shinji Isayama ◽  
Noriyasu Sakamoto ◽  
Kimitoshi Umeda

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