scholarly journals Characterization of N-Terminal Glutamate Cyclization in Monoclonal Antibody and Bispecific Antibody Using Charge Heterogeneity Assays and Hydrophobic Interaction Chromatography

2021 ◽  
Author(s):  
Mingyan Cao ◽  
Niluka De Mel ◽  
Jihong Wang ◽  
Conner Parthemore ◽  
Yang Jiao ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Hydrophobic Interaction ◽  
Bispecific Antibody ◽  
Hydrophobic Interaction Chromatography ◽  
Charge Heterogeneity

Purification, immobilization and characterization of thermostable α-amylase from a thermophilic bacterium Geobacillus sp. TF14

2017 ◽  
Vol 42 (6) ◽  
Author(s):  
Şaban Keskin ◽  
Nagihan Saglam Ertunga
Keyword(s):  
Ion Exchange ◽  
Hydrophobic Interaction ◽  
Hydrophobic Interaction Chromatography ◽  
Maximal Activity ◽  
Thermophilic Bacterium ◽  
Single Band ◽  
Sds Page

AbstractObjective:In this study, α-amylase from a thermophilic bacterium Geobacillus sp. TF14 was purified and immobilized on two different supports.Methods:Ion exchange and hydrophobic interaction chromatography techniques were employed for the purification.Results:The enzyme was purified as 17.11 fold and determined as a single band of 54 kDa on SDS-PAGE. Purified enzyme showed two pH optimums of pH 5.00 and pH 9.00 and the enzyme is quite stable at these pHs over a period of 48 h. Purified enzyme showed maximal activity at 75°C and stability at this temperature over a period of 72 h. It was observed that CaConclusion:It can be concluded that the purified enzyme may find application in many fields of starch based industries.

Prediction of protein retention times in hydrophobic interaction chromatography by robust statistical characterization of their atomic-level surface properties

2016 ◽  
Vol 32 (2) ◽  
pp. 372-381 ◽  
Author(s):  
Alexander T. Hanke ◽  
Marieke E. Klijn ◽  
Peter D. E. M. Verhaert ◽  
Luuk A. M. van der Wielen ◽  
Marcel Ottens ◽  
...  
Keyword(s):  
Surface Properties ◽  
Hydrophobic Interaction ◽  
Hydrophobic Interaction Chromatography ◽  
Atomic Level ◽  
Level Surface ◽  
Statistical Characterization ◽  
Protein Retention

Preparation and Characterization of Cellulose Based Adsorbents for Large Scale Hydrophobic Interaction Chromatography

1994 ◽  
Vol 17 (4) ◽  
pp. 749-760 ◽  
Author(s):  
A. Šėrys ◽  
J. Liesienė ◽  
J. Urbonavičienė ◽  
A. Maruška ◽  
K. Radzevičius ◽  
...  
Keyword(s):  
Hydrophobic Interaction ◽  
Large Scale ◽  
Hydrophobic Interaction Chromatography
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