scholarly journals Nuclear RNA purification by flow cytometry to study nuclear processes in plants

2021 ◽  
Vol 2 (1) ◽  
pp. 100320
Author(s):  
Belén Moro ◽  
Malgorzata Kisielow ◽  
Veronica Barragan Borrero ◽  
Antoine Bouet ◽  
Christopher A. Brosnan ◽  
...  
1990 ◽  
Vol 22 (3) ◽  
pp. 187-193 ◽  
Author(s):  
Thomas C. Chen ◽  
Devron H. Char ◽  
Frederick Waldman ◽  
Richard P. Juster

2021 ◽  
Author(s):  
Jaya Rammohan ◽  
Nina Alperovich ◽  
Bin Shao ◽  
David Ross

RNA degradation plays a major role in cellular function, but current methods for measuring RNA degradation require RNA purification or are low throughput. Here we show how a flow-FISH assay can be used for high-throughput, in situ measurement of RNA degradation without RNA purification. We demonstrate how this approach can be used to simultaneously measure RNA degradation rates of different RNA sequences in a single assay and explore how the assay can be used to examine the effect of cellular context on RNA degradation rates. This assay will be generally useful to quantitatively measure how natural and engineered biological function depends on RNA half-life.


1976 ◽  
Vol 32 ◽  
pp. 169-182
Author(s):  
B. Kuchowicz

SummaryIsotopic shifts in the lines of the heavy elements in Ap stars, and the characteristic abundance pattern of these elements point to the fact that we are observing mainly the products of rapid neutron capture. The peculiar A stars may be treated as the show windows for the products of a recent r-process in their neighbourhood. This process can be located either in Supernovae exploding in a binary system in which the present Ap stars were secondaries, or in Supernovae exploding in young clusters. Secondary processes, e.g. spontaneous fission or nuclear reactions with highly abundant fission products, may occur further with the r-processed material in the surface of the Ap stars. The role of these stars to the theory of nucleosynthesis and to nuclear physics is emphasized.


2001 ◽  
Vol 66 (2) ◽  
pp. 100-106 ◽  
Author(s):  
M. Bellido ◽  
E. Rubiol ◽  
J. Ubeda ◽  
O. Lopez ◽  
C. Estivill ◽  
...  

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