TNF-α (rs1800629) polymorphism modifies the effect of sensitization to house dust mite on asthma and bronchial hyperresponsiveness in children

2020 ◽  
Vol 115 ◽  
pp. 104467
Author(s):  
Eun Lee ◽  
So-Yeon Lee ◽  
Min Jee Park ◽  
Soo-Jong Hong
Keyword(s):  
House Dust ◽  
House Dust Mite ◽  
Bronchial Hyperresponsiveness ◽  
Tnf Α ◽  
Dust Mite

scholarly journals Biologic Responses to House Dust Mite Exposure in the Environmental Exposure Unit

2022 ◽  
Vol 2 ◽  
Author(s):  
Lubnaa Hossenbaccus ◽  
Sophia Linton ◽  
Jenny Thiele ◽  
Lisa Steacy ◽  
Terry Walker ◽  
...  
Keyword(s):  
Environmental Exposure ◽  
House Dust ◽  
House Dust Mite ◽  
Dermatophagoides Pteronyssinus ◽  
Allergen Exposure ◽  
Healthy Controls ◽  
Dermatophagoides Farinae ◽  
Post Exposure ◽  
Tnf Α ◽  
Dust Mite

Introduction: Allergic rhinitis (AR) is an inflammatory disease of the nasal mucosa that can be modeled using Controlled Allergen Exposure Facilities (CACF). Recently, we clinically validated the house dust mite (HDM) Environmental Exposure Unit (EEU) facility. In the current study, we aimed to assess biological responses in the blood following HDM exposure in the HDM-EEU.Methods: Fifty-five participants passed a screening visit, where they provided consent and completed a skin prick test (SPT), then attended a modest or higher HDM exposure session. Baseline and post-exposure blood samples were collected. Complete blood counts with differentials were measured, and isolated serum was used to determine Dermatophagoides farinae- and Dermatophagoides pteronyssinus-specific IgE (sIgE) and cytokine concentrations (IL-4, IL-5, IL-6, IL-10, IL-13, TNF-α).Results: HDM-allergic participants had significantly greater SPT wheal sizes than healthy controls. sIgE concentrations were significantly greater in allergic participants, with a strong correlation between Dermatophagoides farinae and Dermatophagoides pteronyssinus. Serum eosinophil counts were significantly decreased post-exposure for allergic participants. White blood cell, neutrophil, and lymphocyte counts were significantly increased for both allergic and non-allergic participants post-exposure. Serum IL-13 concentrations were significantly reduced post-exposure in allergics while TNF-α was significantly reduced in non-allergics.Conclusion: The HDM-EEU is a useful model for investigating biologic mechanisms of HDM-induced AR. Allergic participants produced measurable biological changes compared to healthy controls following allergen exposure, specifically with serum expression of eosinophils and related markers, namely IL-5, which promotes the proliferation and differentiation of eosinophils, and IL-13, a cytokine released by eosinophils. The exact mechanisms at play require further investigation.

scholarly journals The effect of sensitization to house dust mite on the asthma development is modified by TNF-α (rs1800629) polymorphism

2020 ◽  
Vol 145 (2) ◽  
pp. AB176
Author(s):  
Eun Lee ◽  
So-Yeon Lee ◽  
Min Jee Park ◽  
Eun Sang Rhee ◽  
Soo-Jong Hong
Keyword(s):  
House Dust ◽  
House Dust Mite ◽  
Tnf Α ◽  
Dust Mite

scholarly journals Sensitization by subcutaneous route is superior to intraperitoneal route in induction of asthma by house dust mite in a murine mode

2015 ◽  
Vol 13 (4) ◽  
pp. 560-566 ◽  
Author(s):  
Marcelo Vivolo Aun ◽  
Beatriz Mangueira Saraiva-Romanholo ◽  
Francine Maria de Almeida ◽  
Thayse Regina Brüggemann ◽  
Jorge Kalil ◽  
...  
Keyword(s):  
House Dust ◽  
House Dust Mite ◽  
Immunoglobulin E ◽  
Airway Remodeling ◽  
Bronchial Hyperresponsiveness ◽  
Specific Ige ◽  
Negative Control ◽  
Therapeutic Drugs ◽  
Specific Immunoglobulin E ◽  
Dust Mite

ABSTRACT Objective To develop a new experimental model of chronic allergic pulmonary disease induced by house dust mite, with marked production of specific immunoglobulin E (IgE), eosinophilic inflammatory infiltrate in the airways and remodeling, comparing two different routes of sensitization. Methods The protocol lasted 30 days. BALB/c mice were divided into six groups and were sensitized subcutaneously or intraperitoneally with saline (negative control), Dermatophagoides pteronyssinus (Der p) 50 or 500mcg in three injections. Subsequently they underwent intranasal challenge with Der p or saline for 7 days and were sacrificed 24 hours after the last challenge. We evaluated the titration of specific IgE anti-Der p, eosinophilic density in peribronchovascular space and airway remodeling. Results Both animals sensitized intraperitoneally and subcutaneously produced specific IgE anti-Der p. Peribronchovascular eosinophilia increased only in mice receiving lower doses of Der p. However, only the group sensitized with Der p 50mcg through subcutaneously route showed significant airway remodeling. Conclusion In this murine model of asthma, both pathways of sensitization led to the production of specific IgE and eosinophilia in the airways. However, only the subcutaneously route was able to induce remodeling. Furthermore, lower doses of Der p used in sensitization were better than higher ones, suggesting immune tolerance. Further studies are required to evaluate the efficacy of this model in the development of bronchial hyperresponsiveness, but it can already be replicated in experiments to create new therapeutic drugs or immunotherapeutic strategies.

Clinical Efficacy and Possible Mechanism of Endoscopic Vidian Neurectomy for House Dust Mite-Sensitive Allergic Rhinitis

ORL ◽  
2021 ◽  
pp. 1-10
Author(s):  
Li Shen ◽  
Jun Wang ◽  
Xue Kang ◽  
Mei Han ◽  
Mengyue Li ◽  
...  
Keyword(s):  
Allergic Rhinitis ◽  
Clinical Efficacy ◽  
House Dust ◽  
House Dust Mite ◽  
Immunoglobulin E ◽  
Serum Levels ◽  
Vidian Neurectomy ◽  
Tnf Α ◽  
Dust Mite

<b><i>Background/Aims:</i></b> Endoscopic vidian neurectomy (EVN) for allergic rhinitis (AR) has good clinical effects. However, the pathophysiological basis of the effect of EVN on AR is still poorly understood. This study aimed to investigate the efficacy of EVN on house dust mite (HDM)-sensitive AR and the dynamic changes of serum immunoglobulin E and some immune regulatory factors. <b><i>Methods:</i></b> Twenty HDM-sensitive AR patients were treated with bilateral EVN (EVN group), 15 HDM-sensitive AR patients were treated with subcutaneous immunotherapy (SCIT group), and 15 healthy subjects served as healthy controls. Quality of daily life was assessed by the scores of the Rhinoconjunctivitis Quality of Life Questionnaire (RQLQs). The visual analog scale was used to assess clinical efficacy. Serum molecules were measured by ELISA and the UNICAP system. <b><i>Results:</i></b> Compared with the SCIT group, the RQLQs in the EVN group were lower 12 months after treatment (both <i>p</i> &#x3c; 0.05). There was no significant difference in improving nasal itching and sneezing (both <i>p</i> &#x3e; 0.05), but the clinical efficacy of bilateral EVN was greater than SCIT in improving nasal obstruction, rhinorrhea, eye itching, and lachrymation 12 months after treatment (all <i>p</i> &#x3c; 0.05). Compared with before treatment, the serum levels of total immunoglobulin E (tIgE), <i>Dermatophagoides pteronyssinus</i>- and <i>Dermatophagoides farinae</i>-specific immunoglobulin E (sIgE), and tumor necrosis factor (TNF)-α in the EVN group and the serum levels of TNF-α and interleukin-4 in the SCIT group were lower 12 months after treatment (all <i>p</i> &#x3c; 0.05). <b><i>Conclusion:</i></b> The short-term efficacy of bilateral EVN is more effective than SCIT in treating HDM-sensitive AR. This may be because the surgery reduced the tIgE and sIgE levels. TNF-α may be involved in the therapeutic mechanism.

scholarly journals House Dust Mite-Derived Chitin Enhances Th2 Cell Response to Inhaled Allergens, Mainly via a TNF-α-Dependent Pathway

2016 ◽  
Vol 8 (4) ◽  
pp. 362 ◽  
Author(s):  
Jun-Pyo Choi ◽  
Sang-Min Lee ◽  
Hyun-Il Choi ◽  
Min-Hye Kim ◽  
Seong Gyu Jeon ◽  
...  
Keyword(s):  
House Dust ◽  
House Dust Mite ◽  
Cell Response ◽  
Th2 Cell ◽  
Tnf Α ◽  
Dust Mite ◽  
Dependent Pathway
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