Detection of Chlamydia trachomatis and Trichomonas vaginalis by polymerase chain reaction in introital specimens from pregnant women

1996 ◽  
Vol 175 (1) ◽  
pp. 165-167 ◽  
Author(s):  
Steven S. Witkin ◽  
Steven R. Inglis ◽  
Margaret Polaneczky
Keyword(s):  
Polymerase Chain Reaction ◽  
Chlamydia Trachomatis ◽  
Pregnant Women ◽  
Trichomonas Vaginalis ◽  
Chain Reaction ◽  
Polymerase Chain

Detection of Chlamydia trachomatis in Pregnant Women by the Papanicolaou Technique, Enzyme Immunoassay and Polymerase Chain Reaction

2000 ◽  
Vol 44 (2) ◽  
pp. 114-123 ◽  
Author(s):  
Carmen Adriana Bañuelos Pánuco ◽  
Irma Deleón Rodríguez ◽  
José Tomás Hernández Méndez ◽  
Lydia Alejandra Martínez Guzmán ◽  
David Akle Fierro ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Chlamydia Trachomatis ◽  
Pregnant Women ◽  
Enzyme Immunoassay ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals CLINICAL AND IMMUNOLOGICAL CHANGES OF NON-GONOCOCCAL URETHRITIS

2020 ◽  
Vol 22 (1) ◽  
pp. 40-44
Author(s):  
Gadoev Maruf ◽  
◽  
Bakhromuddin Saidzoda ◽  
Keyword(s):  
Polymerase Chain Reaction ◽  
Chlamydia Trachomatis ◽  
Trichomonas Vaginalis ◽  
Ureaplasma Urealyticum ◽  
Control Group ◽  
Direct Immunofluorescence ◽  
Chain Reaction ◽  
Immunological Disorders ◽  
Gonococcal Urethritis ◽  
Polymerase Chain

Objective: To study the clinical features and the state of general immunological reactivity in non-gonococcal urethritis (NGU) in men. Methods: Examined 100 men between the ages of 20 and 48 years: 75 patients of NGU (main group) and 25 healthy (control group). The average age of the patients was 26.7±1.7 years, and the male of control group was 27.9±1.7 years. Clinical, microscopic, immunological research methods were used, including direct immunofluorescence (DIF), polymerase chain reaction (PCR). Results: Ureaplasma urealyticum was found in 37 (49.3%) patients, 33 (44%) had Chlamydia trachomatis, 23 (30.7%) had Mycoplasma genitalium, 16 (21.3%) had Trichomonas vaginalis. In 24 (32%) of NGU patients had a mixed infection: in 14 (18.7%) had a combination of two STIs and in 10 (13, 3%) had three infections. In 51 (68%) of patients the process passed in the form of monoinfection. Various complaints (dysuric disorders, pain, discomfort and agglutination of the labium urethra) were presented by 51 (68%) of sick patients. The excretions from the urethra were marked in 46 (61.3%) of patients, reproductive disorders are 3 times less common. Immunological disorders were manifested by a decrease in CD4 and CD8 lymphocytes, PHA, PN and IL-10, increase – IgM, IgG, CIC, TNFα, IL-1β. Conclusions: The most common cause of NGU is Ureaplasma urealyticum and Chlamydia trachomatis. In most cases NGU proceeds in the form of monoinfection. Subjective and objective symptoms occur in 64% and 59% of patients, respectively. Immunological disorders were detected in 71% of patients. Keywords: Non-gonogococcal urethritis, direct immunofluorescence, immunoenzyme method, polymerase chain reaction

scholarly journals Detection ofTrichomonas vaginalisUsing the Polymerase Chain Reaction in Pregnant and Non-Pregnant Women

1994 ◽  
Vol 2 (1) ◽  
pp. 16-19 ◽  
Author(s):  
J. Jeremias ◽  
D. Draper ◽  
M. Ziegert ◽  
W. Jones ◽  
S. Inglis ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Pregnant Women ◽  
Trichomonas Vaginalis ◽  
Chain Reaction ◽  
Pcr Assay ◽  
Vaginal Infections ◽  
Wet Mount ◽  
Polymerase Chain ◽  
Asymptomatic Women ◽  
Culture Positive

Objective:Trichomonas vaginalisvaginal infections are often both asymptomatic and difficult to detect by current methods. We evaluated the ability of a newly developed polymerase chain reaction (PCR) assay to identifyT. vaginalisin vaginal samples from pregnant and non-pregnant women.Methods:In the 1st study, we compared the prevalence ofT. vaginalisdetection by PCR and culture using Diamond's medium in 52 women with symptoms of vaginal infection. In the 2nd study,T. vaginaliswas detected using PCR and wet mount microscopy in 131 asymptomatic pregnant women.Results:Among the women with symptoms of vaginitis, 7 (13.5%) were PCR-positive forT. vaginalis. Six of the PCR-positive women, but none of the PCR-negative women, were culture-positive for this organism. All but 1 of the women with candidal vaginitis or bacterial vaginosis were PCR-negative for T.vaginalis. Among the asymptomatic pregnant women, all of whom were negative forT. vaginalisby wet mount, l0 (7.6%) were PCR-positive forT. vaginalis.Conclusions:PCR offers a rapid and sensitive alternative to culture and microscopy for the detection ofT. vaginalisvaginal infections in both symptomatic and asymptomatic women.

scholarly journals Non-detection of Chlamydia trachomatis infection by polymerase chain reaction in pregnant Iranian women

2012 ◽  
Vol 3 (1) ◽  
pp. 8 ◽  
Author(s):  
Parvin Hassanzadeh ◽  
Hosein Sharifi ◽  
Abdollah Bazargani ◽  
Reza Khashei ◽  
Amir Emami ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Chlamydia Trachomatis ◽  
Pregnant Women ◽  
Sensitivity And Specificity ◽  
Urine Samples ◽  
World Health ◽  
Chain Reaction ◽  
Transmitted Infection ◽  
Polymerase Chain ◽  
Pcr Test

<em>Chlamydia trachomatis</em> is the most common cause of sexually transmitted infection. In 75% of women and 50% of men infection is asymptomatic. According to World Health Organization reports, the number of new genital infections with <em>Chlamydia trachomatis</em> reaches 100 million annually. The sensitivity and specificity of nacid amplification tests are 95% and 99%, respectively. Urine samples can provide a non-invasive method of testing for the detection of <em>Chlamydia trachomatis</em> by polymerase chain reaction (PCR). The aim of this study was to evaluate the prevalence of <em>Chlamydia trachomatis</em> infection in pregnant women referred to a teaching hospital affiliated to Shiraz University of Medical Sciences. Urine samples were obtained from 210 pregnant women and investigated microscopically and macroscopically by urinalysis. Precipitants were also used for DNA extraction and PCR test for detecting <em>Chlamydia trachomatis.</em> Among 210 urine specimens from women aged 15-39 years, none were positive for <em>Chlamydia trachomatis </em>by PCR. In spite of the high sensitivity and specificity of PCR, and the elimination of inhibitory effects on PCR test, no pregnant woman was positive for <em>Chlamydia trachomatis</em>. Here, we suggest that a larger sample should be studied and other sensitive methods could also be used in the future.

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