Gas chromatographic determination of isocitric and malic acid in the presence of a large excess of citric acid

1990 ◽  
Vol 239 ◽  
pp. 165-170 ◽  
Author(s):  
I. Molnár-Perl ◽  
M. Morvai ◽  
M. Pintér-Szakács ◽  
M. Petró-Turza
Keyword(s):  
Citric Acid ◽  
Malic Acid ◽  
Chromatographic Determination ◽  
Large Excess

scholarly journals CHROMATOGRAPHIC DETERMINATION OF THE ACIDS OF THE CITRIC ACID CYCLE IN TISSUES

1951 ◽  
Vol 193 (1) ◽  
pp. 277-283 ◽  
Author(s):  
CharlesE. Frohman ◽  
JamesM. Orten ◽  
ArthurH. Smith
Keyword(s):  
Citric Acid ◽  
Citric Acid Cycle ◽  
Chromatographic Determination ◽  
Acid Cycle

Colorimetric and Gas-Liquid Chromatographic Determination of Atropine-Hyoscyamine and Hyoscine (Scopolamine) in Solanaceous Plants

1975 ◽  
Vol 58 (5) ◽  
pp. 884-887
Author(s):  
Mohamed S Karawya ◽  
Samia M Abdel-Wahab ◽  
Mohamed S Hifnawy ◽  
Mohamed G Ghourab
Keyword(s):  
Citric Acid ◽  
Acetic Anhydride ◽  
Thin Layer ◽  
Chromatographic Determination ◽  
Chromatographic Procedure ◽  
Liquid Chromatographic Determination ◽  
Solanaceous Plants ◽  
Liquid Chromatographic ◽  
Colorimetric Methods

Abstract Two colorimetric micromethods are described for the determination of atropine-hyoscyamine and hyoscine (scopolamine), using p-dimethylaminobenzaldehyde and citric acid-acetic anhydride as the color reagents. These methods are sensitive to 60-1200 and 10-360 μg alkaloid/10 ml. The colorimetric methods were also successfully applied after a preliminary thin layer chromatographic separation of the alkaloids. A gasliquid chromatographic procedure was also developed, which yielded comparable results with the colorimetric methods.

Simultaneous Gas Chromatographic Determination of Carboxylic Acids in Soft Drinks and Jams

1985 ◽  
Vol 68 (5) ◽  
pp. 902-905
Author(s):  
Taizo Tsuda ◽  
Hiroshi Nakanishi ◽  
Takashi Morita ◽  
Junko Takebayashi
Keyword(s):  
Benzoic Acid ◽  
Succinic Acid ◽  
Tartaric Acid ◽  
Carboxylic Acids ◽  
Fumaric Acid ◽  
Malic Acid ◽  
Sorbic Acid ◽  
Chromatographic Determination ◽  
Dehydroacetic Acid

Abstract A method was developed for simultaneous gas chromatographic determination of sorbic acid, dehydroacetic acid, and benzoic acid used as preservatives, and succinic acid, fumaric acid, malic acid, and tartaric acid used as acidulants in soft drinks and jams. A sample was dissolved in NH4OH-NH4CI pH 9 buffer solution, and an aliquot of the solution was passed through a QAE-Sephadex A 25 column. The column was washed with water, and the carboxylic acids were eluted with 0.1N HC1. Sorbic acid, dehydroacetic acid, and benzoic acid were extracted with ethyl ether-petroleum ether (1 + 1), and determined on a 5% DEGS + 1% H3PO4 column. Succinic acid, fumaric acid, malic acid, and tartaric acid in the lower layer were derivatized with N,0- bis(trimethylsilyl)acetamide and trimethylchlorosilane, and determined on a 3% SE-30 column. Recoveries from soft drink and jam samples fortified with 0.1% each of 7 carboxylic acids ranged from 92.4 to 102.6% for preservatives, and from 88.1 to 103.2% for acidulants.

Ligand-exchange ion chromatographic determination of malic acid enantiomers in apple juice with photometric detection

1991 ◽  
Vol 585 (2) ◽  
pp. 315-317 ◽  
Author(s):  
Atsushi Yamamoto ◽  
Akinobu Matsunaga ◽  
Eiichi Mizukami ◽  
Kazuichi Hayakawa ◽  
Motoichi Miyazaki
Keyword(s):  
Malic Acid ◽  
Ligand Exchange ◽  
Apple Juice ◽  
Chromatographic Determination ◽  
Photometric Detection

scholarly journals Determination of polydextrose as a fat replacer in butter

2008 ◽  
Vol 25 (No. 1) ◽  
pp. 25-31 ◽  
Author(s):  
K. Míčková ◽  
J. Čopíková ◽  
A. Synytsya
Keyword(s):  
Citric Acid ◽  
Ir Spectroscopy ◽  
Petroleum Ether ◽  
Solid Fraction ◽  
Condensation Product ◽  
Large Excess ◽  
Fat Replacer ◽  
Ft Ir ◽  
Ft Ir Spectroscopy

Polydextrose is used in several countries as a low caloric sugar and fat substitute (bulking agent). It is prepared by condensation of glucose, D-glucitol, and citric acid (89:10:1). The resulting condensation product has no chemically defined structure but it represents a mixture of polymerisation products. The determination of polydextrose in butter is complicated owing to a large excess of fats and to the presence of other compounds, mainly proteins. FT-IR spectroscopy seems to be a satisfying method for the detection of polydextrose in samples derived from food products. The presence of polydextrose in butter was verified after the removal of fats by extraction with petroleum ether and deproteinisation with Sevag reagent, CHCl3/<i>n</i>-butanol (v/v = 4:1) mixture, or trichlorocetic acid. The solid fraction of butter and butter containing a known amount of polydextrose were prepared and analysed by FT-IR spectroscopy. IR marker bands of polydextrose centred at 1150, 1076 and 1040 cm<sup>&minus;1</sup> were found only in the case of the sample of butter with polydextrose.

Determination of Tartaric Acid and the Sum of Malic and Citric Acids in Grape Juices by Potentiometric Titration

1988 ◽  
Vol 71 (5) ◽  
pp. 1028-1032
Author(s):  
Oswaldo E S Godinho ◽  
Nilson E Desouza ◽  
Luiz M Aleixo ◽  
Ari U Ivaska
Keyword(s):  
Citric Acid ◽  
Tartaric Acid ◽  
Potentiometric Titration ◽  
Malic Acid ◽  
Grape Juice ◽  
Algebraic Method ◽  
The Individual ◽  
Grape Juices ◽  
Linear Algebraic Method

Abstract Application of a linear algebraic method to the potentiometric titration of a mixture of tartaric and malic acids makes it possible to determine the individual concentrations of both acids in the same sample. These 2 acids have also been determined in grape juice free of citric acid after their separation from the juice matrix by precipitation as barium salts, followed by selective solubilization. It is also possible to determine tartaric acid and the sum of malic acid and citric acid in grape juice when the latter is present.

scholarly journals Separation and Determination of Some Organic Acids in Dry Calyces of Iraqi Hibiscus Sabdariffa Linn

2015 ◽  
Vol 12 (2) ◽  
pp. 340-349
Author(s):  
Baghdad Science Journal
Keyword(s):  
Acetic Acid ◽  
Citric Acid ◽  
Oxalic Acid ◽  
Organic Acids ◽  
Formic Acid ◽  
Succinic Acid ◽  
Tartaric Acid ◽  
Malic Acid ◽  
Limit Of Detection

A new reversed phase- high performance liquid chromatographic (RP-HPLC) method with Ultraviolet-Visible spectrophotometry has been optimized and validated for the simultaneous extraction and determination of organic acids present in Iraqi calyces of Hibiscus Sabdraffia Linn. The method is based on using ultrasonic bath for extracting organic acids. Limit of detection in µg/ml of Formic acid, Acetic acid, Oxalic acid, Citric acid, Succinic acid, Tartaric acid, and Malic acid 126.8498×10-6, 113.6005×10-6, 97.0513×10-6, 49.7925×10-6, 84.0753×10-6, 92.6551×10-6, and 106.1633×10-6 ,respectively. The concentration of organic acids found in dry spacemen of calyces of Iraqi Hibiscus Sabdraffia Linn. under study: Formic acid, Acetic acid, Oxalic acid, Citric acid, Succinic acid, Tartaric acid, and Malic acid are 114.896 µg/g, 64.722 µg/g, 342.508 µg/g, 126.902 µg/g, 449.91 µg/g, 268.52 µg/g, and 254.07 µg/g respectively.

scholarly journals Identification and determination of polybasic organic acids present in West Indian cherries (malpighia punicifolia L.) and in three varieties of guava (psidium guajava)

1969 ◽  
Vol 37 (3) ◽  
pp. 195-198
Author(s):  
Rafael Santini, Jr.
Keyword(s):  
Ascorbic Acid ◽  
Citric Acid ◽  
Organic Acid ◽  
Malic Acid ◽  
Dehydroascorbic Acid ◽  
West Indian ◽  
Psidium Guajava ◽  
The West ◽  
Acid Lactone

Citric acid was found to be the polybasic organic acid present in large amounts in guavas. Therefore its determination cannot be used as a test for adulteration of guava jelly. Tartaric acid, as well as levo-malic acid, was also present in small amounts in the three varieties of guavas analyzed. The only polybasic organic acid found in the West Indian cherry was leyo-malic acid. Monobasic acid, ascorbic acid, and dehydroascorbic acid (lactone form) were also present in considerable quantities.

Simultaneous Determination of Organic Acids and Vitamin C in Green Beans by Liquid Chromatography

1994 ◽  
Vol 77 (4) ◽  
pp. 1056-1059 ◽  
Author(s):  
M L Vazquez Oderiz ◽  
M E Vazquez Blanco ◽  
J Lopez Hernandez ◽  
J Simal Lozano ◽  
M A Romero Rodriguez
Keyword(s):  
Liquid Chromatography ◽  
Citric Acid ◽  
Vitamin C ◽  
Oxalic Acid ◽  
Organic Acids ◽  
Fumaric Acid ◽  
Malic Acid ◽  
Green Beans ◽  
Coefficients Of Variation

Abstract A method is described for determining and quantitating organic acids (oxalic, malic, citric, and fumaric) and vitamin C by liquid chromatography with a UV–visible detector that allows simultaneous monitoring at 2 wavelengths. The method was applied to samples of green beans (Phaseolus vulgaris L.). Recoveries were 97.8% for oxalic acid, 98.9% for malic acid, 98.7% for citric acid, 99.2% for fumaric acid, and 98.5% for vitamin C. Method precisions (coefficients of variation) were 1.7% for oxalic acid, 0.8% for malic acid, 0.9% for citric acid, 1.5% for fumaric acid, and 1.2% for vitamin C. Measurement precisions (coefficients of variation) were 1.32% for oxalic acid, 0.33% for malic acid, 0.62% for citric acid, 1.01 % for fumaric acid, and 0.39% for vitamin C. Limits of detection were 0.025 mg/mL for oxalic acid, 0.022 mg/mL for malic acid, 0.024 mg/mL for citric acid, 1.0 × 10−4 mg/mL for fumaric acid, and 2.7 × 10−4 mg/mL for vitamin C.

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