Short-end injection procedure in capillary electrophoresis for determination of enzymatic reaction kinetics

2001 ◽  
Vol 441 (1) ◽  
pp. 15-21 ◽  
Author(s):  
X. Cahours ◽  
C. Viron ◽  
Ph. Morin ◽  
I. Renimel ◽  
P. André ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Reaction Kinetics ◽  
Enzymatic Reaction ◽  
Injection Procedure

Determination of Mutation of C-myc Oncogene in Gastric Cancer by Capillary Electrophoresis

2012 ◽  
Vol 39 (11) ◽  
pp. 1695-1700
Author(s):  
Xi-Hui XIE ◽  
Rong WANG ◽  
Zheng-Ping JIA ◽  
Hua XIE ◽  
Ai-Mei ZHAMG ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Capillary Electrophoresis ◽  
Myc Oncogene

Determination of gambogic acid inGambogeby non-aqueous capillary electrophoresis

2015 ◽  
Vol 33 (2) ◽  
pp. 152
Author(s):  
Wanlu OU ◽  
Yujuan LI ◽  
Dongdong SHI ◽  
Feng QU
Keyword(s):  
Capillary Electrophoresis ◽  
Gambogic Acid

Enzymatic colorimetry of lecithin and sphingomyelin in aqueous solution.

1983 ◽  
Vol 29 (8) ◽  
pp. 1513-1517 ◽  
Author(s):  
M W McGowan ◽  
J D Artiss ◽  
B Zak
Keyword(s):  
Aqueous Solution ◽  
Hydrogen Peroxide ◽  
Amniotic Fluid ◽  
Enzymatic Reaction ◽  
Detergent Solution ◽  
Enzymatic Determination ◽  
Red Color ◽  
Hydrolysis Of ◽  
Zwitterionic Detergent

Abstract A procedure for the enzymatic determination of lecithin and sphingomyelin in aqueous solution is described. The phospholipids are first dissolved in chloroform:methanol (2:1 by vol), the solvent is evaporated, and the residue is redissolved in an aqueous zwitterionic detergent solution. The enzymatic reaction sequences of both assays involve hydrolysis of the phospholipids to produce choline, which is then oxidized to betaine, thus generating hydrogen peroxide. The hydrogen peroxide is subsequently utilized in the enzymatic coupling of 4-aminoantipyrine and sodium 2-hydroxy-3,5-dichlorobenzenesulfonate, an intensely red color being formed. The presence of a non-reacting phospholipid enhances the hydrolysis of the reacting phospholipid. Thus we added lecithin to the sphingomyelin standards and sphingomyelin to the lecithin standards. This precise procedure may be applicable to determination of lecithin and sphingomyelin in amniotic fluid.

scholarly journals Determination of Levels of NO-3, NO-2 and NH+4 Ions in Leaves of Various Plants by Capillary Electrophoresis

1996 ◽  
Vol 37 (6) ◽  
pp. 878-880 ◽  
Author(s):  
Y. Kawamura ◽  
M. Takahashi ◽  
G. Arimura ◽  
T. Isayama ◽  
K. Irifune ◽  
...  
Keyword(s):  
Capillary Electrophoresis
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