RESULTS OF HYDRODYNAMIC SYSTEM RESEARCH MILKING OF MILKING INSTALLATIONS

The aim of the research is to develop a mathematical model of the hydraulic injector of the flushing system and to determine the design parameters of the injector that ensure the destruction of milk deposits on the surface of the elements of the milking parlor of complex geometric configuration. A physical and mathematical model of the process of operation of the hydraulic injector of the flushing system has been developed, which creates a jet of detergent solution of directed action for cleaning the inner surfaces of the milk system. As a result of theoretical researches dependences of radius of a site of blow of a stream Ra and its pressure on a surface of milk pipe system pa on internal diameter of a nozzle dn, distance to it Hj at various values of pressure on an exit of a nozzle pn are received. Using the element base of the milking equipment, a low-pressure centrifugal type milk pump (≈ 3 atm) was selected as the basic hydraulic pressure generator. The dn = 2.1 mm, Hj = 16.6 cm, Ra = 23.6 mm. As a result of experimental studies of the hydroinjector of the flushing system, the dependences of the value of the force of the cleaning solution jet on the surface Fa and the degree of cleaning of the surface from milk deposits after flushing χ from the nozzle outlet pressure pn, the distance between the nozzle and the surface Hj and its inner diameter dn. Visual and statistical (according to Fisher's criterion F = 1,68 <F0,05 (6,30) = 2,42 and correlation coefficient R = 0,97) comparison of theoretical and experimental results of researches allows to assert about adequacy and logic of the received dependences of force of action. jet of detergent solution on the surface of Fa from the research factors in the appropriate range of their values. Pn = 500 kPa, dn = 0.00175 m, Hj = 0.191 m were determined as rational design and mode parameters of the hydroinjector from the condition of the maximum degree of cleaning of the surface from milk deposits after washing χ = 40.1%.

FORMULATION AND IMPROVEMENT OF TECHNOLOGICAL SOLUTIONS FOR WASTEWATER TREATMENT OF AGRICULTURAL REPAIR ENTERPRISES

The article presents the main production cycles of engine repair at a repair and mechanical plant. The main production cycle of engine repair at the repair and mechanical plant consists of external steaming of the unit in the steaming chamber with the discharge of the formed effluent into the pre-fabricated well. Next the disassembly of the en-gine into individual components is carried out, then they are cleaned in cross washing machines with periodic discharge of spent detergent solutions into the prefabricated tanks. After restoration of details the assembly of the diesel engine and its test on stands is carried out. The necessary parts are electrochemically galvanized, chrome-plated or coated with iron. Several local wastewater treatment schemes on the plant with utilization of valuable components and their reuse are offered. Wastewater and waste process solutions are conventionally divided into low-concentrated and concentrated. Low-concentrated wastewater includes effluents from the diesel test site, flushing water from plating baths, and discharge of a revolving diesel cooling system. A scheme for wastewater treatment from petrole-um products and substances in the form of suspensions has been developed and researched, which allows reusing wastewater from the re-verse water supply to the diesel test site. The schematic diagram of the reverse water supply of the diesel test area will consist of a column electrical flotation coagulator, contaminated water collectors, saturators and pumps. A scheme of electrochemical purification of waste detergents has been developed, which includes receivers of detergent solution, preliminary settling of coarse impurities, column electrical coagulator-floater with soluble aluminum electrodes, electrolyte collection. The peculiarity of the device is the operation of the electrode system in a pure electrolyte, which eliminates the possibility of contamination and passivation of electrical and chemical processes. The electrically generated coagulant is dosed into the reaction chamber, mixed with the detergent solution, coagulates and floats the contamination, which allows to extend the service life of the detergent solutions in two or three times.

PENERAPAN PEWARNA ALAMI BIJI ALPUKAT PADA KAIN KATUN

ABSTRAKBiji alpukat dapat diekstrak menjadi zat warna alam karena mengandung senyawa tanin yang dapat menimbulkan warna cokelat atau kecokelatan. Kelemahan zat warna alam dalam proses pewarnaan tekstil yakni memiliki ketahanan luntur yang rendah. Oleh karena itu, pada proses pencelupan bahan tekstil dengan zat warna alam dibutuhkan proses fiksasi agar warna memiliki ketahanan luntur yang baik. Pewarna alami dari biji alpukat telah diterapkan pada kain katun. Ketahanan luntur pewarna alami telah diuji dengan memvariasikan massa tawas sebagai bahan fiksasi terhadap nilai perubahan intensitas cahaya pada air residu hasil pencucian kain katun. Perlakuan menggunakan variasi massa tawas 10g hingga 60g dengan waktu pencelupan selama 10 menit. Desain penelitian terdiri dari ekstraksi biji alpukat sebagai sumber zat warna alam, mordanting dan pencelupan kain katun ke dalam larutan zat warna, fiksasi kain katun, dan pengujian ketahanan luntur warna pada kain katun menggunakan luxmeter setelah pencucian menggunakan air biasa dan air deterjen. Hasil penelitian menunjukkan bahwa sampel yang menggunakan massa tawas 60g menghasilkan tingkat kelunturan warna paling kecil dengan nilai perubahan intensitas cahaya 9,58lux pada hasil pencucian dengan air biasa dan 3,11lux pada hasil pencucian dengan air deterjen. Semakin tinggi massa tawas yang digunakan sebagai bahan fiksasi pada kain katun, maka pewarna alami biji alpukat memiliki ketahanan luntur yang semakin baik. Penggunaan sampel tawas 60g sebagai bahan fiksasi efektif dalam menjaga ketahanan luntur pewarna alami biji alpukat pada kain katun. Kata kunci: Biji Alpukat; Kain Katun; Pewarna Alami; Tawas. ABSTRACTAvocado seeds could be extracted into natural dyes because they contained of tannin. Tannin as a dyes substances would cause brown or brownish color. The weakness of natural dyes in the textile dyeing process that has a low fade resistance. Therefore, the dyeing process of textile materials with natural dyes were needed fixation process in order that color had a well fade resistance. The natural dyes from avocado seeds had been applicated on cotton textile. The fade resistance of natural dyes had been tested by varying the mass of Al2(SO4)3as a fixation to the value of light intensity difference on the residue water of the cotton textile washing. The treatment used the mass variation of 10g to 60g Al2(SO4)3by dyeing for 10 minutes. The study design consisted of avocado seeds extraction as a source of natural dyes, mordanting of cotton textile, dyeing of cotton textile in a dye solution, fixation of cotton textile and test the fade resistance of natural dyes on cotton textile using a luxmeter after washing by water and detergent solution. The results showed that the sample used a mass of 60g Al2(SO4)3produced the smallest color fade degree with the value of light intensity difference on the residue water was 9.58lux of washing by water and 3.11lux of washing by detergent solution. The higher mass of Al2(SO4)3was used as fixation on cotton textile, the natural dyes of avocado seeds had a better fade resistance. The using of 60g Al2(SO4)3sample as a fixation material was effective for kept the fade resistance of natural dyes from avocado seeds on cotton textile. Keywords: Avocado Seeds; Cotton Textile; Natural Dyes.

Interior vs. Exterior Incorporation of Silver Nanoparticles in Cotton Fiber and Washing Durability

The popular use of silver nanoparticles (Ag NPs) in commercial textile products that inhibit odor- and/or infection-causing bacteria has continuously raised concerns about their washing durability. The poor durability not only deteriorates the antibacterial performance, but also results in unwanted leaching of NPs into washing solutions. In this study, we showed how the incorporation location of Ag NPs—interior vs. exterior of cotton fiber—governs their durability against consecutive simulated home launderings. The superior washing durability of interior NPs was confirmed. The Ag losses after 50 laundering cycles for interior and exterior systems were 16% and 63% in water and 24% and 78% in detergent solution, respectively. The cotton fabric containing interior NPs predominantly released ionic Ag, whereas the fabric containing exterior NPs released particulate Ag.

Assessment of the efficiency of different chemical treatments and ultrasonic cleaning for defatting of cancellous bone samples

Our study aimed to asses the defatting efficiency of different methods, which are commonly used and easily available in the laboratory in order to find a method that is effective, convenient, safe, and economical. Cylindrical cancellous bone specimens were obtained from fresh-frozen human cadaver femoral condyles, cut into multiple small specimens (Ø8 × 2 mm), and assigned to two groups that were treated with either chemical solvent soaking (Solvent group) or ultrasonic cleaning (Ultrasound group). Each group was divided into several subgroups based on different treatments. Digital photographs were taken of each specimen. The difference of material density (Δρb), apparent density (Δρapp), and porosity (ΔP) before and after treatment were used as evaluation indicators. For the solvent group, in Δρb, only the combination of 99% ethanol and detergent solution showed a significant difference before and after treatment (P = 0.00). There was no significant difference in ΔP among acetone, the mixture of 99% ethanol and acetone, and the combination of 99% ethanol and detergent solution (P = 0.93). For the ultrasound group, the median of all subgroups in Δρapp and ΔP were all lower than the solvent group. The combination of 99% ethanol and detergent solution (v/v = 1:20), as well as the mixture of 99% ethanol and acetone (v/v = 1:1), seem to be the optimal defatting methods for 2 mm thick cancellous bone slices due to their effectiveness, availability, low-cost and safety. Chemical soaking for 24 h is more effective than ultrasonic cleaning with 99% ethanol or acetone for 20 or 40 min.

PSI-3 An assessment of the barrier function of canine skin after repeated decontamination

Working canines are often deployed to environments with unknown chemical and biological contaminants. Deployed canines may operate in highly contaminated disaster sites for lengthy periods of time requiring daily decontamination efforts. The skin provides a barrier by retaining moisture and preventing entry by contaminants and pathogens. However, few data exist on the impact of repeated decontamination to the canine skin. The objective of this study was to identify changes in dermal health during and after a 14-day serial decontamination program. Labrador retrievers (n = 8) were decontaminated daily using a dish detergent solution (1:8, detergent:water). Skin measurements were collected weekly for pH, trans-epidermal water loss (TEWL), sebum, and moisture. Additionally, visual assessments were recorded for skin health, coat condition, and dander scores (back and body). Statistical tests were conducted with SAS (version 9.4) with measurements analyzed using a PROC GLM Two Way ANOVA and visual assessments analyzed using PROC FREQ Chi Square test. Significance was set at 5% for all tests. Repeated decontamination significantly increased TEWL (P &lt; 0.0001) through day 16. Sebum content was also impacted by repeated daily decontamination efforts (P = 0.0387). Sebum decreased initially before steadily rising. In contrast, moisture content (P = 0.3842) and pH (P = 0.7462), were unaffected by repeated decontamination. Interestingly, dander scores assessed on the back were worsened by repeated decontamination (P = 0.0222) but dander scores assessed across the whole body were unaffected (P = 0.1804). Coat shine was unaffected by decontamination (P = 0.1156) similar to coat softness (P = 0.3418). Overall coat condition remained unchanged as a result of repeated decontamination efforts (P = 0.9466). These data reveal that daily decontamination impacts dermal function, potentially risks for canines working in contaminated areas. Future work should include investigations into methods for decontamination to mitigate these risks.

Kinetic mechanism of Na+-coupled aspartate transport catalyzed by GltTk

It is well-established that the secondary active transporters GltTk and GltPh catalyze coupled uptake of aspartate and three sodium ions, but insight in the kinetic mechanism of transport is fragmentary. Here, we systematically measured aspartate uptake rates in proteoliposomes containing purified GltTk, and derived the rate equation for a mechanism in which two sodium ions bind before and another after aspartate. Re-analysis of existing data on GltPh using this equation allowed for determination of the turnover number (0.14 s−1), without the need for error-prone protein quantification. To overcome the complication that purified transporters may adopt right-side-out or inside-out membrane orientations upon reconstitution, thereby confounding the kinetic analysis, we employed a rapid method using synthetic nanobodies to inactivate one population. Oppositely oriented GltTk proteins showed the same transport kinetics, consistent with the use of an identical gating element on both sides of the membrane. Our work underlines the value of bona fide transport experiments to reveal mechanistic features of Na+-aspartate symport that cannot be observed in detergent solution. Combined with previous pre-equilibrium binding studies, a full kinetic mechanism of structurally characterized aspartate transporters of the SLC1A family is now emerging.

Dispersion of Carbon Nanotubes with “Green” Detergents

Solubilization of carbon nanotubes (CNTs) is a fundamental technique for the use of CNTs and their conjugates as nanodevices and nanobiodevices. In this work, we demonstrate the preparation of CNT suspensions with “green” detergents made from coconuts and bamboo as fundamental research in CNT nanotechnology. Single-walled CNTs (SWNTs) with a few carboxylic acid groups (3–5%) and pristine multi-walled CNTs (MWNTs) were mixed in each detergent solution and sonicated with a bath-type sonicator. The prepared suspensions were characterized using absorbance spectroscopy, scanning electron microscopy, and Raman spectroscopy. Among the eight combinations of CNTs and detergents (two types of CNTs and four detergents, including sodium dodecyl sulfate (SDS) as the standard), SWNTs/MWNTs were well dispersed in all combinations except the combination of the MWNTs and the bamboo detergent. The stability of the suspensions prepared with coconut detergents was better than that prepared with SDS. Because the efficiency of the bamboo detergents against the MWNTs differed significantly from that against the SWNTs, the natural detergent might be useful for separating CNTs. Our results revealed that the use of the “green” detergents had the advantage of dispersing CNTs as well as SDS.

Insights into detoxification of tolaasins, the toxins behind mushroom bacterial blotch, by Microbacterium foliorum NBRC 103072T

Tolaasins are lipodepsipeptides secreted by Pseudomonas tolaasii, the causal agent of brown blotch disease of mushrooms, and are the toxins that cause the brown spots. We previously reported that Microbacterium foliorum NBRC 103072T is an effective tolaasin-detoxifying bacterium. In this study, we aimed to characterize the tolaasin-detoxification process of M. foliorum NBRC 103072T. The tolaasin-detoxification by M. foliorum NBRC 103072T was carried out by hydrolyzation of tolaasins at two specific sites in the peptide moiety of tolaasins by its cells, and the resulting fragments were released from bacterial cells. The tolaasin-hydrolyzing activity can be extracted by neutral detergent solution from M. foliorum NBRC 103072T cells. Moreover, tolaasin-adsorption to the bacterial cells occurred prior to hydrolyzation of tolaasins, which might contribute to the effective tolaasin-detoxification by M. foliorum NBRC 103072T. It is notable that the tolaasin-degradation process by M. foliorum NBRC 103072T is carried out by hydrolyzation at specific sites in the peptide moiety of lipopeptide by bacterial cells as a novel biological degradation process of cyclic lipopeptides.

Determination of Reasonable Value Range of Some Parameters When Cleaning Steel with Ultrasonic Assistance

There are many parameters affecting the amount of removed rust on the steel surface during ultrasonic cleaning. This article presents the experimental study results to determine the value range of some paratmeters of machining process to ensure the amount of removed rust on the steel surface with great value. Tests were performed in two different detergent solution media of without using acid and using acid. The reasonable value ranges of machine power, machining time, distance from steel plate to ultrasonic transducer (called machining distance) and detergent solution concentration have been determined. From the results achieved in this paper, the development direction for the next studies has also been proposed.