Simultaneous spectrophotometric determination of chromium(VI) and iron(III) with chromogenic mixed reagents by H-point standard addition method and partial least squares regression

2001 ◽  
Vol 442 (2) ◽  
pp. 327-336 ◽  
Author(s):  
H. Abdollahi
Keyword(s):  
Least Squares ◽  
Partial Least Squares ◽  
Spectrophotometric Determination ◽  
Partial Least Squares Regression ◽  
Standard Addition ◽  
Standard Addition Method ◽  
Least Squares Regression ◽  
Addition Method ◽  
Chromium Vi

scholarly journals Simultaneous spectrophotometric determination of valsartan and hydrochlorothiazide by H-point standard addition method and partial least squares regression

2011 ◽  
Vol 61 (1) ◽  
pp. 37-50 ◽  
Author(s):  
Karunanidhi Lakshmi ◽  
Sivasubramanian Lakshmi
Keyword(s):  
Least Squares ◽  
Partial Least Squares ◽  
Spectrophotometric Determination ◽  
Partial Least Squares Regression ◽  
Standard Addition ◽  
Standard Addition Method ◽  
Calibration Model ◽  
Least Squares Regression ◽  
Addition Method

Simultaneous spectrophotometric determination of valsartan and hydrochlorothiazide by H-point standard addition method and partial least squares regression Simultaneous determination of valsartan and hydrochlorothiazide by the H-point standard additions method (HPSAM) and partial least squares (PLS) calibration is described. Absorbances at a pair of wavelengths, 216 and 228 nm, were monitored with the addition of standard solutions of valsartan. Results of applying HPSAM showed that valsartan and hydrochlorothiazide can be determined simultaneously at concentration ratios varying from 20:1 to 1:15 in a mixed sample. The proposed PLS method does not require chemical separation and spectral graphical procedures for quantitative resolution of mixtures containing the titled compounds. The calibration model was based on absorption spectra in the 200-350 nm range for 25 different mixtures of valsartan and hydrochlorothiazide. Calibration matrices contained 0.5-3 μg mL-1 of both valsartan and hydrochlorothiazide. The standard error of prediction (SEP) for valsartan and hydrochlorothiazide was 0.020 and 0.038 μg mL-1, respectively. Both proposed methods were successfully applied to the determination of valsartan and hydrochlorothiazide in several synthetic and real matrix samples.

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