scholarly journals Characterization of a heat modifiable protein, Escherichia coli outer membrane protein OmpA in binary surfactant system of sodium dodecyl sulfate and octylglucoside

1998 ◽  
Vol 1375 (1-2) ◽  
pp. 101-109 ◽  
Author(s):  
Satoshi Ohnishi ◽  
Keiichi Kameyama ◽  
Toshio Takagi
Keyword(s):  
Escherichia Coli ◽  
Sodium Dodecyl Sulfate ◽  
Membrane Protein ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
Sodium Dodecyl ◽  
Surfactant System ◽  
Dodecyl Sulfate

Characterization of a Phage Specific to Hemorrhagic Escherichia coli  O157:H7 and Disclosure of Variations in Host Outer Membrane Protein OmpC

1998 ◽  
Vol 5 (5) ◽  
pp. 370-382 ◽  
Author(s):  
Sung-Liang Yu ◽  
Hwia-Cheng Ding ◽  
June-Nam Seah ◽  
Kang-Mai Wu ◽  
Yu-Chung Chang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Membrane Protein ◽  
Outer Membrane ◽  
Outer Membrane Protein

scholarly journals Characterization of Outer Membrane Protein OmpU of Vibrio cholerae O1

1998 ◽  
Vol 66 (10) ◽  
pp. 4726-4728 ◽  
Author(s):  
Noboru Nakasone ◽  
Masaaki Iwanaga
Keyword(s):  
Membrane Protein ◽  
Outer Membrane ◽  
Vibrio Cholerae ◽  
Outer Membrane Protein ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Rabbit Small Intestine ◽  
Vibrio Cholerae O1

ABSTRACT The outer membrane protein OmpU of Vibrio cholerae O1 strain 86B3 was characterized with reference to colonization of the intestine by the organism. The purified OmpU exhibited a pI of 3.6. Upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis, it migrated to 38, 32, and 110 kDa when the sample was heated at 100°C for 2 min, 50°C for 15 min, and room temperature for 30 min, respectively. The purified OmpU was not hemagglutinative. Anti-OmpU serum did not agglutinate strain 86B3 or other V. cholerae organisms. OmpU adhered to the brush border of the rabbit small intestine; adhesion of the organisms to the intestine treated in advance with OmpU was not inhibited. Treating the organisms in advance with anti-OmpU Fab did not inhibit adhesion to the intestine. These results obtained in vitro suggest that OmpU is not involved in the adhesion of V. cholerae to the intestinal epithelium.

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