scholarly journals Metal ion dependence of a heat-modifiable protein from the outer membrane of Escherichia coli upon sodium dodecyl sulfate-gel electrophoresis.

1977 ◽  
Vol 132 (1) ◽  
pp. 314-320 ◽  
Author(s):  
J C McMichael ◽  
J T Ou
Keyword(s):  
Escherichia Coli ◽  
Sodium Dodecyl Sulfate ◽  
Outer Membrane ◽  
Gel Electrophoresis ◽  
Metal Ion ◽  
Sodium Dodecyl ◽  
Dodecyl Sulfate

scholarly journals mraY Is an Essential Gene for Cell Growth in Escherichia coli

1998 ◽  
Vol 180 (23) ◽  
pp. 6429-6432 ◽  
Author(s):  
David S. Boyle ◽  
William D. Donachie
Keyword(s):  
Escherichia Coli ◽  
Cell Growth ◽  
Sodium Dodecyl Sulfate ◽  
Gel Electrophoresis ◽  
Essential Gene ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Polyacrylamide Gel ◽  
Dodecyl Sulfate

ABSTRACT The synthesis of the murein precursor lipid I is performed by MraY. We have shown that mraY is an essential gene for cell growth. Cells depleted of MraY first swell and then lyse. The expression of mraY DNA in vitro produces a 40-kDa polypeptide detectable by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

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