Partial amino acid sequence analysis of human placenta monoamine oxidase A and bovine liver monoamine oxidase B

1988 ◽  
Vol 156 (1) ◽  
pp. 445-450 ◽  
Author(s):  
Shiuan Chen ◽  
Walter Weyler
Keyword(s):  
Amino Acid ◽  
Sequence Analysis ◽  
Amino Acid Sequence ◽  
Monoamine Oxidase ◽  
Human Placenta ◽  
Bovine Liver ◽  
Monoamine Oxidase A ◽  
Monoamine Oxidase B ◽  
Amino Acid Sequence Analysis ◽  
Partial Amino Acid Sequence

scholarly journals Monoamine oxidase A from human placenta and monoamine oxidase B from bovine liver both have one FAD per subunit

1989 ◽  
Vol 260 (3) ◽  
pp. 725-729 ◽  
Author(s):  
W Weyler
Keyword(s):  
Amino Acid ◽  
Monoamine Oxidase ◽  
Bovine Liver ◽  
Spectrophotometric Titration ◽  
Monoamine Oxidase A ◽  
Monoamine Oxidase B ◽  
Enzyme Preparations ◽  
Function Mechanism ◽  
Upper Limit ◽  
Quantitative Amino Acid Analysis

I present the first clear evidence that the protein: FAD ratio in human monoamine oxidase A and bovine monoamine oxidase B has an upper limit of 65 kDa and 57 kDa per FAD, respectively. To now it had been assumed that the protein: FAD ratio was 100-120 kDa to 1 FAD and that there was one FAD per two subunits which were assumed to be of the same size. For the present work the purity of monoamine oxidase A and monoamine oxidase B was improved over that previously achieved. Protein was determined by quantitative amino acid analysis and FAD content was measured by spectrophotometric titration of SDS-denatured enzyme with NaS2O4 standardized against riboflavin. The cause of the previous misassignment of the protein: FAD ratio was judged as having been due to the use of impure enzyme preparations. Knowledge of the correct protein: FAD ratio is important in devising cloning strategies for this enzyme, in understanding its structure, function, mechanism, and in the studies of its biosynthesis.

scholarly journals Purification and partial amino acid sequence analysis of human erythrocyte acetylcholinesterase

FEBS Letters ◽  
1989 ◽  
Vol 247 (2) ◽  
pp. 279-282 ◽  
Author(s):  
Vijay Chhajlani ◽  
Dwight Derr ◽  
Betty Earles ◽  
Eli Schmell ◽  
Thomas August
Keyword(s):  
Amino Acid ◽  
Sequence Analysis ◽  
Amino Acid Sequence ◽  
Human Erythrocyte ◽  
Amino Acid Sequence Analysis ◽  
Partial Amino Acid Sequence
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