A new assay for thyroglobulin concentration in serum using monoclonal antibodies against synthetic peptides

2000 ◽  
Vol 298 (1-2) ◽  
pp. 69-84 ◽  
Author(s):  
Ryoji Kato ◽  
Masayuki Maruyama ◽  
Tetsuo Sekino ◽  
Yoshio Kasuga
Keyword(s):  
Monoclonal Antibodies ◽  
Synthetic Peptides ◽  
Thyroglobulin Concentration

New monoclonal antibodies as probes for human cardiac troponin I: Epitopic analysis with synthetic peptides

1992 ◽  
Vol 29 (2) ◽  
pp. 271-278 ◽  
Author(s):  
Catherine Larue ◽  
Hélène Defacque-Lacquement ◽  
Charles Calzolari ◽  
Dung Le Nguyen ◽  
Bernard Pau
Keyword(s):  
Monoclonal Antibodies ◽  
Cardiac Troponin ◽  
Synthetic Peptides ◽  
Troponin I ◽  
Cardiac Troponin I

Characterization of Murine Monoclonal Antibodies to HIV-1 Induced by Synthetic Peptides

1988 ◽  
Vol 4 (5) ◽  
pp. 331-342 ◽  
Author(s):  
PAUL J. DURDA ◽  
BARBARA LEECE ◽  
ANNA JENOSKI ◽  
HARVEY RABIN ◽  
AMANDA FISHER ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Synthetic Peptides ◽  
Murine Monoclonal Antibodies ◽  
Hiv 1

Epitope Mapping of Monoclonal Antibodies to Keratin 19 Using Keratin Fragments, Synthetic Peptides and Phage Peptide Libraries

1995 ◽  
Vol 231 (2) ◽  
pp. 475-485 ◽  
Author(s):  
Volker Bottger ◽  
Pauline C. Stasiak ◽  
Dolores L. Harrison ◽  
Dervla M. Mellerick ◽  
E. Birgitte Lane
Keyword(s):  
Monoclonal Antibodies ◽  
Synthetic Peptides ◽  
Epitope Mapping ◽  
Peptide Libraries ◽  
Keratin 19

scholarly journals Detection of isoform 4 of the plasma membrane calcium pump in human tissues by using isoform-specific monoclonal antibodies

1996 ◽  
Vol 316 (1) ◽  
pp. 353-359 ◽  
Author(s):  
Ariel J. CARIDE ◽  
Adelaida G. FILOTEO ◽  
Agnes ENYEDI ◽  
Anil K. VERMA ◽  
John T. PENNISTON
Keyword(s):  
Monoclonal Antibodies ◽  
Plasma Membrane ◽  
Human Brain ◽  
Synthetic Peptides ◽  
Heart Tissue ◽  
Calcium Pump ◽  
Human Tissues ◽  
Western Blots ◽  
Overlapping Bands

The epitope location and specificity of monoclonal antibodies JA9, 5F10 and JA3, raised against the human plasma membrane Ca2+ pump (hPMCA), were analysed by using synthetic peptides of the corresponding epitopes as well as the complete isoforms, hPMCA4b, hPMCA4a and hPMCA1b, expressed in COS-1 cells. The experiments with the peptides showed that JA9 reacted specifically with a region containing residues 51–75 of hPMCA4 (a or b), but not with the same region of isoforms 1, 2 or 3. JA3 reacted with residues 1156–1180, a region unique to hPMCA4b. 5F10 reacted in the region of residues 719–738, which is highly conserved in all PMCA isoforms. Indeed, 5F10 recognized all three isoforms expressed in COS-1 cells. JA9, in contrast, reacted with both variants a and b of hPMCA4 but not with hPMCA1, and JA3 recognized exclusively hPMCA4b. We used these antibodies to discern the distribution of hPMCA4a and hPMCA4b in human brain, heart, kidney and lung. In Western blots of human brain samples, we could identify both hPMCA4a and hPMCA4b. Heart tissue also showed isoform 4b, and probably 4a. In contrast, kidney and lung showed primarily hPMCA4b. In brain, overlapping bands that did not correspond to either variant of hPMCA4 were detected, and in kidney a band migrating in the same position as hPMCA1b was observed. The distribution of the a and b forms of hPMCA4 at the protein level, as analysed by these antibodies, is consistent with the available data about the abundance of mRNAs for the hPMCA isoforms. The presence of hPMCA4b in all the samples supports the proposed role of this isoenzyme as a constitutive form of the pump.

Sign in / Sign up

Export Citation Format

Share Document