Biochemical stabilization enhances red blood cell recovery and stability following cryopreservation

Cryobiology ◽  
2002 ◽  
Vol 45 (2) ◽  
pp. 153-166 ◽  
Author(s):  
Christopher T Wagner ◽  
Melissa L Martowicz ◽  
Stephen A Livesey ◽  
Jerome Connor
Transfusion ◽  
2019 ◽  
Vol 59 (7) ◽  
pp. 2264-2275 ◽  
Author(s):  
Richard O. Francis ◽  
Sonia Mahajan ◽  
Francesca Rapido ◽  
Francesca La Carpia ◽  
Mark Soffing ◽  
...  

Blood ◽  
2007 ◽  
Vol 110 (11) ◽  
pp. 4932-4932
Author(s):  
Shi Nae Kim ◽  
Sang Kyun Sohn ◽  
Jong Gwang Kim ◽  
Yee Soo Kim ◽  
Yoon Young Cho ◽  
...  

Abstract Background: Granulocyte colony-stimulating-factor (G-CSF) is widely used for the mobilization of hematopoietic stem cells from normal donors. It has been reported that the addition of granulocyte-macrophage CSF (GM-CSF) to G-CSF mobilized more primitive CD34+ subsets than did G-CSF alone. We investigated the effects of combination regimen (GM-CSF + G-CSF + darbepoetin-α) for mobilization in normal healthy donors and compared its efficacy in mobilizing stem cells with G-CSF alone. Methods: Thirty donors were randomly assigned to one of the following regimens for mobilization: G-CSF group (G-CSF 5ug/kg/day for 5 to 7 days) and combination group (GM-CSF 10ug/kg/day on 1st and 2nd days, G-CSF 5ug/kg/day for 5 to 7 days and 40mg of dalbepoetin-α on day 1) Results: The counts of MNC and CD34+ cells/kg were not significantly different between two groups (p=0.129 and p=0.651). The CD3+ cell counts per kilogram were 3.48±1.45×108/kg and 2.54±0.94×108/kg in a G-CSF group and a combination group, respectively (p=0.04). Red blood cell transfusion requirement was not different between two groups (p=0.469). The median time to neutrophil and platelet engraftment was 12.5 vs. 12.0 days (p=0.443) and 11.5 vs. 11.0 days (p=0.752). Red blood cell recovery was 7.5 days and 7 days in G-CSF group and combination group, respectively (p=0.231). Grade II–IV acute graft-versus-host disease (GVHD) was observed in 20% in G-CSF group and 13% in combination group (p=0.50). Conclusion: The combination regimen was not superior to G-CSF alone in terms of acute GVHD and CD34+ cell dose. But CD3+ cell count was significantly lower in combination regimen group. The role of lower CD3+ cells in the graft need to be elucidated in the future.


2017 ◽  
Vol 20 (3) ◽  
pp. 11-13
Author(s):  
André Ibrahim David ◽  
Catiana Mitica Gritti ◽  
André Gustavo Santos Pereira ◽  
Tiago Emanuel de Souza ◽  
Felipe Sbrolini Borges ◽  
...  

Introduction: Liver transplantation is considered the best treatment for irreversible liver disease. Due to the complexity of the procedure generally multiple blood transfusions are required. One option to reduce this need is the use of Cell Saver providing blood cell recovery and reinfusion. Purpose: to evaluate mortality in the number of washed red blood cell units recovered by Cell Saver and perioperative mortality. Methods: Data collection of transplant patients using CellSaver on the period from July 2014 to February 2017. Results: Performed 56 liver transplants, the diagnosis prevalent was liver cirrhosis by hepatitis C virus (37,5%). Of these 45 (80,4%) of the transplants used Cell Saver, which had a median MELD of 24,5 (range 7-50). Taking into account the use of Cell Saver in washed red blood cell units and recovered notes a median of four (range 1-45). 12 died (26,7%), the average of these MELD was 25,5 (range 1-50) and the use of washed red blood cell and recovered was a median of 4,5. Conclusion: Patients who died used a similar amount of washed and recovered erythrocyte units.


Transfusion ◽  
2017 ◽  
Vol 58 (2) ◽  
pp. 352-358 ◽  
Author(s):  
Demet Nalbant ◽  
José A. Cancelas ◽  
Donald M. Mock ◽  
Svetlana V. Kyosseva ◽  
Robert L. Schmidt ◽  
...  

2005 ◽  
Vol 23 (2) ◽  
pp. 283-294 ◽  
Author(s):  
Jonathan H. Waters

Vox Sanguinis ◽  
1999 ◽  
Vol 76 (4) ◽  
pp. 237-240 ◽  
Author(s):  
Paolo Perutelli ◽  
Silvia Catellani ◽  
Lucia Scarso ◽  
Paolo Cornaglia-Ferraris ◽  
Giorgio Dini

Transfusion ◽  
2015 ◽  
Vol 55 (8) ◽  
pp. 1972-1979
Author(s):  
Michael Bellone ◽  
Huy P. Pham ◽  
Beth H. Shaz ◽  
Patricia A. Shi

2015 ◽  
Vol 44 (2) ◽  
pp. 188-193 ◽  
Author(s):  
Andrew G. Burton ◽  
Kaitlin C. Clark ◽  
Dori L. Borjesson ◽  
Danielle D. Carrade ◽  
Julie Burges ◽  
...  

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