Pyridine Nucleotide Pattern and Reduction Charge in Wheat Seedlings with Special Regard to Different Photosynthetic Conditions

1985 ◽  
Vol 180 (1) ◽  
pp. 1-14 ◽  
Author(s):  
G. Peine ◽  
P. Hoffmann ◽  
G. Seifert ◽  
G. Schilling
1980 ◽  
Vol 26 (3) ◽  
pp. 405-408 ◽  
Author(s):  
L. Führer ◽  
C. P. Kubicek ◽  
M. Röhr

A reliable method for the extraction and assay of pyridine nucleotides (NAD, NADH, NADP, and NADPH) in filamentous fungi is presented. The method is applied to a study of the physiology of citric acid accumulation by Aspergillus niger. The measurement of "catabolic reduction charge" (NADH/(NAD + NADH)) and "anabolic reduction charge" (NADPH/(NADP + NADPH)) as a means of characterizing the metabolic state is discussed.


2014 ◽  
Vol 63 (2) ◽  
pp. 167-171 ◽  
Author(s):  
Gabriela Lorenc-Plucińska ◽  
Piotr Karolewski

After prolonged (3-9 weeks) hydroponic treatment of Scots pine seedlings with different concentrations (0.5-4.0 mM) of Al (AI(N0<sub>3</sub>)<sub>3</sub>), the levels of pyridine nucleotides were determined in root homogenates. After 3 weeks of Al stress, a significant decrease of the anabolic reduction charge (ARC: NADPH/(NADP<sup>+</sup> + NADPH)) and an increase of the redox status (NAD(P)H/NAD(P)<sup>+</sup>), catabolic reduction charge (CRC: NADH/(NAD<sup>+</sup> + NADH)) and phosphorylation capacity expressed as NADP<sup>+</sup>/NAD<sup>+</sup> ratio was found in the 4.0 mM Al treatment. After 6 weeks, Al at concentrations of 0.5 and 1.0 mM induced an enhancement of the NADH level and a reduction of NADPH level, but the redox ratios were not changed significantly. After 9 weeks treatment with Al concentrations ranging from 0.5 to 4.0 mM, decreases of the relative level of NADP<sup>+</sup>, NADPH and NADH and increases of NAD<sup>+</sup> were found. Consequently, the CRC, NAD(P)H/NAD(P)<sup>+</sup> and NADP<sup>+</sup>/NAD<sup>+</sup> ratios reached a minimum and ARC a maximum as compared to previous measurements.


2017 ◽  
Vol 69 (1) ◽  
pp. 31-50 ◽  
Author(s):  
Václav Janda ◽  
Martin Kříž ◽  
Tereza Konvalinková ◽  
Jan Borovička
Keyword(s):  

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